Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations

In the present study, the influence of short-term osmotic variations on some electrophysiological properties related to NaCl absorption across posterior gills of Chasmagnathus granulatus was investigated. The transepithelial potential difference (V te ) of isolated and perfused gills increased signi...

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Autores principales:	Tresguerres, M., Onken, H., Pérez, A.F., Luquet, C.M.
Formato:	JOUR
Materias:	Chasmagnathus granulatus Crab Cyclic AMP Gills Hyperosmoregulation Na + /K + -ATPase Perfused gills Short-circuit current Split gill lamellae Transepithelial conductance Transepithelial voltage adenosine triphosphatase (potassium sodium) cyclic AMP sodium chloride animal article Brachyura cell membrane potential drug effect electrophysiology gill male metabolism methodology osmotic pressure physiology Animals Electrophysiology Male Membrane Potentials Na(+)-K(+)-Exchanging ATPase Osmotic Pressure Sodium Chloride Animalia Chasmagnathus granulata Decapoda (Crustacea)
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00220949_v206_n3_p619_Tresguerres
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00220949_v206_n3_p619_Tresguerres2023-10-03T14:26:01Z Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations Tresguerres, M. Onken, H. Pérez, A.F. Luquet, C.M. Chasmagnathus granulatus Crab Cyclic AMP Gills Hyperosmoregulation Na + /K + -ATPase Perfused gills Short-circuit current Split gill lamellae Transepithelial conductance Transepithelial voltage adenosine triphosphatase (potassium sodium) cyclic AMP sodium chloride animal article Brachyura cell membrane potential drug effect electrophysiology gill male metabolism methodology osmotic pressure physiology Animals Brachyura Cyclic AMP Electrophysiology Gills Male Membrane Potentials Na(+)-K(+)-Exchanging ATPase Osmotic Pressure Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) In the present study, the influence of short-term osmotic variations on some electrophysiological properties related to NaCl absorption across posterior gills of Chasmagnathus granulatus was investigated. The transepithelial potential difference (V te ) of isolated and perfused gills increased significantly when hyposmotic saline (699 mosmol l -1 ) was used instead of isosmotic Solution (1045 mosmol l -1 ). A reduction of the concentration of Na + or Cl - at constant osmolarity did not produce any change in V te . Transepithelial short-circuit current (I sc ) and conductance (G te ), measured with split gill lamellae mounted in a modified Ussing chamber, also increased after changing to hyposmotic salines (I sc : from -89.0±40.8 μAcm -2 to -179.3±37.0μAcm -2 ; G te : from 40.5±16.9 mS cm -2 to 47.3±15.8 mS cm -2 ). The observed effects of reduced osmolarity were fast, reversible and gradually dependent on the magnitude of the osmotic variation. The acitivity of the Na + /K + -ATPase increased significantly after perfusion with hyposmotic saline, from 18.73±6.35 μmol P i h -1 mg -1 to 41.84±14.54 μmol P i h -1 mg -1 . Theophylline maintained part of the elevated V te induced by hyposmotic saline, suggesting that an increased cellular cyclic AMP level is involved in the response to reduced osmolarity. In summary, the results indicate that the hemolymph osmolarity regulates active transbranchial NaCl absorption by modulating the activity of the basolateral Na + /K + -ATPase and by changing a conductive pathway, probably at the apical membrane. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220949_v206_n3_p619_Tresguerres
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chasmagnathus granulatus Crab Cyclic AMP Gills Hyperosmoregulation Na + /K + -ATPase Perfused gills Short-circuit current Split gill lamellae Transepithelial conductance Transepithelial voltage adenosine triphosphatase (potassium sodium) cyclic AMP sodium chloride animal article Brachyura cell membrane potential drug effect electrophysiology gill male metabolism methodology osmotic pressure physiology Animals Brachyura Cyclic AMP Electrophysiology Gills Male Membrane Potentials Na(+)-K(+)-Exchanging ATPase Osmotic Pressure Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea)
spellingShingle	Chasmagnathus granulatus Crab Cyclic AMP Gills Hyperosmoregulation Na + /K + -ATPase Perfused gills Short-circuit current Split gill lamellae Transepithelial conductance Transepithelial voltage adenosine triphosphatase (potassium sodium) cyclic AMP sodium chloride animal article Brachyura cell membrane potential drug effect electrophysiology gill male metabolism methodology osmotic pressure physiology Animals Brachyura Cyclic AMP Electrophysiology Gills Male Membrane Potentials Na(+)-K(+)-Exchanging ATPase Osmotic Pressure Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea) Tresguerres, M. Onken, H. Pérez, A.F. Luquet, C.M. Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
topic_facet	Chasmagnathus granulatus Crab Cyclic AMP Gills Hyperosmoregulation Na + /K + -ATPase Perfused gills Short-circuit current Split gill lamellae Transepithelial conductance Transepithelial voltage adenosine triphosphatase (potassium sodium) cyclic AMP sodium chloride animal article Brachyura cell membrane potential drug effect electrophysiology gill male metabolism methodology osmotic pressure physiology Animals Brachyura Cyclic AMP Electrophysiology Gills Male Membrane Potentials Na(+)-K(+)-Exchanging ATPase Osmotic Pressure Sodium Chloride Animalia Brachyura Chasmagnathus granulata Decapoda (Crustacea)
description	In the present study, the influence of short-term osmotic variations on some electrophysiological properties related to NaCl absorption across posterior gills of Chasmagnathus granulatus was investigated. The transepithelial potential difference (V te ) of isolated and perfused gills increased significantly when hyposmotic saline (699 mosmol l -1 ) was used instead of isosmotic Solution (1045 mosmol l -1 ). A reduction of the concentration of Na + or Cl - at constant osmolarity did not produce any change in V te . Transepithelial short-circuit current (I sc ) and conductance (G te ), measured with split gill lamellae mounted in a modified Ussing chamber, also increased after changing to hyposmotic salines (I sc : from -89.0±40.8 μAcm -2 to -179.3±37.0μAcm -2 ; G te : from 40.5±16.9 mS cm -2 to 47.3±15.8 mS cm -2 ). The observed effects of reduced osmolarity were fast, reversible and gradually dependent on the magnitude of the osmotic variation. The acitivity of the Na + /K + -ATPase increased significantly after perfusion with hyposmotic saline, from 18.73±6.35 μmol P i h -1 mg -1 to 41.84±14.54 μmol P i h -1 mg -1 . Theophylline maintained part of the elevated V te induced by hyposmotic saline, suggesting that an increased cellular cyclic AMP level is involved in the response to reduced osmolarity. In summary, the results indicate that the hemolymph osmolarity regulates active transbranchial NaCl absorption by modulating the activity of the basolateral Na + /K + -ATPase and by changing a conductive pathway, probably at the apical membrane.
format	JOUR
author	Tresguerres, M. Onken, H. Pérez, A.F. Luquet, C.M.
author_facet	Tresguerres, M. Onken, H. Pérez, A.F. Luquet, C.M.
author_sort	Tresguerres, M.
title	Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
title_short	Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
title_full	Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
title_fullStr	Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
title_full_unstemmed	Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations
title_sort	electrophysiology of posterior, nacl-absorbing gills of chasmagnathus granulatus: rapid responses to osmotic variations
url	http://hdl.handle.net/20.500.12110/paper_00220949_v206_n3_p619_Tresguerres
work_keys_str_mv	AT tresguerresm electrophysiologyofposteriornaclabsorbinggillsofchasmagnathusgranulatusrapidresponsestoosmoticvariations AT onkenh electrophysiologyofposteriornaclabsorbinggillsofchasmagnathusgranulatusrapidresponsestoosmoticvariations AT perezaf electrophysiologyofposteriornaclabsorbinggillsofchasmagnathusgranulatusrapidresponsestoosmoticvariations AT luquetcm electrophysiologyofposteriornaclabsorbinggillsofchasmagnathusgranulatusrapidresponsestoosmoticvariations
_version_	1807319568478109696

Electrophysiology of posterior, NaCl-absorbing gills of Chasmagnathus granulatus: Rapid responses to osmotic variations

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