Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis

Histamine (HA) is a neurotransmitter synthesized in most mammalian tissues exclusively by histidine decarboxylase enzyme. Among the plethora of actions mediated by HA, the modulatory effects on steroidogenesis and proliferation in Leydig cells (LCs) have been described recently. To determine whether...

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Autores principales:	Pagotto, R.M., Pereyra, E.N., Monzón, C., Mondillo, C., Pignataro, O.P.
Formato:	JOUR
Materias:	Adrenocortical cells Histamine Proliferation Steroidogenesis histamine inositol phosphate adrenal cortex cell animal cell article cell proliferation controlled study drug inhibition G2 phase cell cycle checkpoint human human cell Leydig cell MTT assay nonhuman priority journal steroidogenesis Western blotting adrenocortical cells proliferation Adrenal Cortex Animals Cell Line Cell Proliferation G2 Phase Cell Cycle Checkpoints Humans M Phase Cell Cycle Checkpoints Mice Steroids
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p15_Pagotto
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00220795_v221_n1_p15_Pagotto
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spelling	todo:paper_00220795_v221_n1_p15_Pagotto2023-10-03T14:25:57Z Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis Pagotto, R.M. Pereyra, E.N. Monzón, C. Mondillo, C. Pignataro, O.P. Adrenocortical cells Histamine Proliferation Steroidogenesis histamine inositol phosphate adrenal cortex cell animal cell article cell proliferation controlled study drug inhibition G2 phase cell cycle checkpoint human human cell Leydig cell MTT assay nonhuman priority journal steroidogenesis Western blotting adrenocortical cells histamine proliferation steroidogenesis Adrenal Cortex Animals Cell Line Cell Proliferation G2 Phase Cell Cycle Checkpoints Histamine Humans M Phase Cell Cycle Checkpoints Mice Steroids Histamine (HA) is a neurotransmitter synthesized in most mammalian tissues exclusively by histidine decarboxylase enzyme. Among the plethora of actions mediated by HA, the modulatory effects on steroidogenesis and proliferation in Leydig cells (LCs) have been described recently. To determine whether the effects on LCs reported could be extrapolated to all steroidogenic systems, in this study, we assessed the effect of this amine on adrenal proliferation and steroidogenesis, using two adrenocortical cell lines as experimental models, murine Y1 cells and human NCI-H295R cells. Even when steroidogenesis was not modified by HA in adrenocortical cells, the biogenic amine inhibited the proliferation of H295R cells. This action was mediated by the activation of HRH1 subtype and an increase in the production of inositol phosphates as second messengers, causing cell-cycle arrest in the G2/M phase. These results indicate a new role for HA in the proliferation of human adrenocortical cells that could contribute to a better understanding of tumor pathology as well as to the development of new therapeutic agents. © 2014 Society for Endocrinology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p15_Pagotto
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Adrenocortical cells Histamine Proliferation Steroidogenesis histamine inositol phosphate adrenal cortex cell animal cell article cell proliferation controlled study drug inhibition G2 phase cell cycle checkpoint human human cell Leydig cell MTT assay nonhuman priority journal steroidogenesis Western blotting adrenocortical cells histamine proliferation steroidogenesis Adrenal Cortex Animals Cell Line Cell Proliferation G2 Phase Cell Cycle Checkpoints Histamine Humans M Phase Cell Cycle Checkpoints Mice Steroids
spellingShingle	Adrenocortical cells Histamine Proliferation Steroidogenesis histamine inositol phosphate adrenal cortex cell animal cell article cell proliferation controlled study drug inhibition G2 phase cell cycle checkpoint human human cell Leydig cell MTT assay nonhuman priority journal steroidogenesis Western blotting adrenocortical cells histamine proliferation steroidogenesis Adrenal Cortex Animals Cell Line Cell Proliferation G2 Phase Cell Cycle Checkpoints Histamine Humans M Phase Cell Cycle Checkpoints Mice Steroids Pagotto, R.M. Pereyra, E.N. Monzón, C. Mondillo, C. Pignataro, O.P. Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
topic_facet	Adrenocortical cells Histamine Proliferation Steroidogenesis histamine inositol phosphate adrenal cortex cell animal cell article cell proliferation controlled study drug inhibition G2 phase cell cycle checkpoint human human cell Leydig cell MTT assay nonhuman priority journal steroidogenesis Western blotting adrenocortical cells histamine proliferation steroidogenesis Adrenal Cortex Animals Cell Line Cell Proliferation G2 Phase Cell Cycle Checkpoints Histamine Humans M Phase Cell Cycle Checkpoints Mice Steroids
description	Histamine (HA) is a neurotransmitter synthesized in most mammalian tissues exclusively by histidine decarboxylase enzyme. Among the plethora of actions mediated by HA, the modulatory effects on steroidogenesis and proliferation in Leydig cells (LCs) have been described recently. To determine whether the effects on LCs reported could be extrapolated to all steroidogenic systems, in this study, we assessed the effect of this amine on adrenal proliferation and steroidogenesis, using two adrenocortical cell lines as experimental models, murine Y1 cells and human NCI-H295R cells. Even when steroidogenesis was not modified by HA in adrenocortical cells, the biogenic amine inhibited the proliferation of H295R cells. This action was mediated by the activation of HRH1 subtype and an increase in the production of inositol phosphates as second messengers, causing cell-cycle arrest in the G2/M phase. These results indicate a new role for HA in the proliferation of human adrenocortical cells that could contribute to a better understanding of tumor pathology as well as to the development of new therapeutic agents. © 2014 Society for Endocrinology.
format	JOUR
author	Pagotto, R.M. Pereyra, E.N. Monzón, C. Mondillo, C. Pignataro, O.P.
author_facet	Pagotto, R.M. Pereyra, E.N. Monzón, C. Mondillo, C. Pignataro, O.P.
author_sort	Pagotto, R.M.
title	Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
title_short	Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
title_full	Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
title_fullStr	Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
title_full_unstemmed	Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
title_sort	histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis
url	http://hdl.handle.net/20.500.12110/paper_00220795_v221_n1_p15_Pagotto
work_keys_str_mv	AT pagottorm histamineinhibitsadrenocorticalcellproliferationbutdoesnotaffectsteroidogenesis AT pereyraen histamineinhibitsadrenocorticalcellproliferationbutdoesnotaffectsteroidogenesis AT monzonc histamineinhibitsadrenocorticalcellproliferationbutdoesnotaffectsteroidogenesis AT mondilloc histamineinhibitsadrenocorticalcellproliferationbutdoesnotaffectsteroidogenesis AT pignataroop histamineinhibitsadrenocorticalcellproliferationbutdoesnotaffectsteroidogenesis
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Histamine inhibits adrenocortical cell proliferation but does not affect steroidogenesis

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