Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice

Abnormal exposure to steroid hormones within a critical developmental period elicits permanent alterations in female reproductive physiology in rodents, but the impact on the female GH axis and the underlying sexual differences in hepatic enzymes have not been described in detail. We have investigat...

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Autores principales:	Ramirez, M.C., Luque, G.M., Ornstein, A.M., Becu-Villalobos, D.
Formato:	JOUR
Materias:	growth hormone liver protein messenger RNA somatostatin testosterone propionate androgen therapy animal experiment animal tissue article body weight drug activity female genome imprinting hypothalamus male mouse nonhuman priority journal protein expression protein secretion protein urine level Animals Aryl Hydrocarbon Hydroxylases Body Weight Cytochrome P-450 Enzyme System Female Growth Hormone Hypothalamus Insulin-Like Growth Factor I Liver Male Mice Mice, Inbred C57BL Pituitary Gland Prolactin Proteins Steroid Hydroxylases Testosterone Virilism
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00220795_v207_n3_p301_Ramirez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00220795_v207_n3_p301_Ramirez
record_format	dspace
spelling	todo:paper_00220795_v207_n3_p301_Ramirez2023-10-03T14:25:56Z Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice Ramirez, M.C. Luque, G.M. Ornstein, A.M. Becu-Villalobos, D. growth hormone liver protein messenger RNA somatostatin testosterone propionate androgen therapy animal experiment animal tissue article body weight drug activity female genome imprinting hypothalamus male mouse nonhuman priority journal protein expression protein secretion protein urine level Animals Aryl Hydrocarbon Hydroxylases Body Weight Cytochrome P-450 Enzyme System Female Growth Hormone Hypothalamus Insulin-Like Growth Factor I Liver Male Mice Mice, Inbred C57BL Pituitary Gland Prolactin Proteins Steroid Hydroxylases Testosterone Virilism Abnormal exposure to steroid hormones within a critical developmental period elicits permanent alterations in female reproductive physiology in rodents, but the impact on the female GH axis and the underlying sexual differences in hepatic enzymes have not been described in detail. We have investigated the effect of neonatal androgenization of female mice (achieved by s.c. injection of 100 mg testosterone propionate (TP) on the day of birth: TP females) on the GHRH-somatostatin-GH axis and downstream GH targets, which included female and male predominant liver enzymes and secreted proteins. At 4 months of age, an organizational effect of neonatal testosterone was evidenced on hypothalamic Ghrh mRNA level but not on somatostatin (stt) mRNA level. Ghrh mRNA levels were higher in males than in females, but not in TP females. Increased expression in TP females correlated with increased pituitary GH content and somatotrope population, increased serum and liver IGF-I concentration, and ultimately higher body weight. Murine urinary proteins (MUPs) that were excreted at higher levels in male urine, and whose expression requires pulsatile occupancy of liver GH receptors, were not modified in TP females and neither was liver Mup 1/2/6/8 mRNA expression. Furthermore, a male predominant liver gene (Cyp2d9) was not masculinized in TP females either, whereas two female predominant genes (Cyp2b9 and Cyp2a4) were defeminized. These data support the hypothesis that neonatal steroid exposure contributes to the remodeling of the GH axis and defeminization of hepatic steroid-metabolizing enzymes, which may compromise liver physiology. © 2010 Society for Endocrinology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220795_v207_n3_p301_Ramirez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	growth hormone liver protein messenger RNA somatostatin testosterone propionate androgen therapy animal experiment animal tissue article body weight drug activity female genome imprinting hypothalamus male mouse nonhuman priority journal protein expression protein secretion protein urine level Animals Aryl Hydrocarbon Hydroxylases Body Weight Cytochrome P-450 Enzyme System Female Growth Hormone Hypothalamus Insulin-Like Growth Factor I Liver Male Mice Mice, Inbred C57BL Pituitary Gland Prolactin Proteins Steroid Hydroxylases Testosterone Virilism
spellingShingle	growth hormone liver protein messenger RNA somatostatin testosterone propionate androgen therapy animal experiment animal tissue article body weight drug activity female genome imprinting hypothalamus male mouse nonhuman priority journal protein expression protein secretion protein urine level Animals Aryl Hydrocarbon Hydroxylases Body Weight Cytochrome P-450 Enzyme System Female Growth Hormone Hypothalamus Insulin-Like Growth Factor I Liver Male Mice Mice, Inbred C57BL Pituitary Gland Prolactin Proteins Steroid Hydroxylases Testosterone Virilism Ramirez, M.C. Luque, G.M. Ornstein, A.M. Becu-Villalobos, D. Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
topic_facet	growth hormone liver protein messenger RNA somatostatin testosterone propionate androgen therapy animal experiment animal tissue article body weight drug activity female genome imprinting hypothalamus male mouse nonhuman priority journal protein expression protein secretion protein urine level Animals Aryl Hydrocarbon Hydroxylases Body Weight Cytochrome P-450 Enzyme System Female Growth Hormone Hypothalamus Insulin-Like Growth Factor I Liver Male Mice Mice, Inbred C57BL Pituitary Gland Prolactin Proteins Steroid Hydroxylases Testosterone Virilism
description	Abnormal exposure to steroid hormones within a critical developmental period elicits permanent alterations in female reproductive physiology in rodents, but the impact on the female GH axis and the underlying sexual differences in hepatic enzymes have not been described in detail. We have investigated the effect of neonatal androgenization of female mice (achieved by s.c. injection of 100 mg testosterone propionate (TP) on the day of birth: TP females) on the GHRH-somatostatin-GH axis and downstream GH targets, which included female and male predominant liver enzymes and secreted proteins. At 4 months of age, an organizational effect of neonatal testosterone was evidenced on hypothalamic Ghrh mRNA level but not on somatostatin (stt) mRNA level. Ghrh mRNA levels were higher in males than in females, but not in TP females. Increased expression in TP females correlated with increased pituitary GH content and somatotrope population, increased serum and liver IGF-I concentration, and ultimately higher body weight. Murine urinary proteins (MUPs) that were excreted at higher levels in male urine, and whose expression requires pulsatile occupancy of liver GH receptors, were not modified in TP females and neither was liver Mup 1/2/6/8 mRNA expression. Furthermore, a male predominant liver gene (Cyp2d9) was not masculinized in TP females either, whereas two female predominant genes (Cyp2b9 and Cyp2a4) were defeminized. These data support the hypothesis that neonatal steroid exposure contributes to the remodeling of the GH axis and defeminization of hepatic steroid-metabolizing enzymes, which may compromise liver physiology. © 2010 Society for Endocrinology.
format	JOUR
author	Ramirez, M.C. Luque, G.M. Ornstein, A.M. Becu-Villalobos, D.
author_facet	Ramirez, M.C. Luque, G.M. Ornstein, A.M. Becu-Villalobos, D.
author_sort	Ramirez, M.C.
title	Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
title_short	Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
title_full	Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
title_fullStr	Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
title_full_unstemmed	Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice
title_sort	differential neonatal testosterone imprinting of gh-dependent liver proteins and genes in female mice
url	http://hdl.handle.net/20.500.12110/paper_00220795_v207_n3_p301_Ramirez
work_keys_str_mv	AT ramirezmc differentialneonataltestosteroneimprintingofghdependentliverproteinsandgenesinfemalemice AT luquegm differentialneonataltestosteroneimprintingofghdependentliverproteinsandgenesinfemalemice AT ornsteinam differentialneonataltestosteroneimprintingofghdependentliverproteinsandgenesinfemalemice AT becuvillalobosd differentialneonataltestosteroneimprintingofghdependentliverproteinsandgenesinfemalemice
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Differential neonatal testosterone imprinting of GH-dependent liver proteins and genes in female mice

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