Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production
The present study was designed to investigate the effect of lipopolysaccharide (LPS) on the expression levels and activities of the nitric oxide synthase (NOS) and heme oxygenase (HO) systems in the rat adrenal gland. Both enzymatic activities were significantly increased in this tissue after in viv...
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todo:paper_00220795_v194_n1_p11_Grion2023-10-03T14:25:55Z Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production Grion, N. Repetto, E.M. Pomeraniec, Y. Martinez Calejman, C. Astort, F. Sanchez, R. Pignataro, O.P. Arias, P. Cymeryng, C.B. bacterium lipopolysaccharide corticotropin endotoxin heme oxygenase inducible nitric oxide synthase n(g) nitroarginine methyl ester neuronal nitric oxide synthase protoporphyrin tin adrenal function animal experiment animal tissue article controlled study enzyme activity enzyme analysis enzyme inhibition genetic code hormone action hormone determination immunomodulation in vivo study male nonhuman priority journal protein expression rat signal transduction steroidogenesis Adrenal Cortex Adrenocorticotropic Hormone Animals Corticosterone Enzyme Activation Enzyme Inhibitors Gene Expression Regulation, Bacterial Heme Oxygenase-1 Lipopolysaccharides Male Metalloporphyrins NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Nitric Oxide Synthase Type II Protoporphyrins Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Stimulation, Chemical The present study was designed to investigate the effect of lipopolysaccharide (LPS) on the expression levels and activities of the nitric oxide synthase (NOS) and heme oxygenase (HO) systems in the rat adrenal gland. Both enzymatic activities were significantly increased in this tissue after in vivo treatment with LPS. The concurrent induction of the HO-1, NOS-1, and NOS-2 gene products was also detected as both mRNAs and protein levels were augmented by this treatment in a time-dependent way. A significant interaction between both signaling systems was also demonstrated as in vivo blockage of NOS activity with N(G)-nitro-L-arginine methyl ester (L-NAME) resulted in a significant reduction in HO expression and activity levels, while an increase in NOS activity was observed when HO was inhibited by Sn-protoporphyrin IX (Sn-PPIX). As both NOS and HO activities have been previously involved in the modulation of adrenal steroidogenesis, we investigated the participation of these signaling systems in the adrenal response to LPS. Our results showed that acute stimulation of steroid production by ACTH was significantly increased when either NOS or HO activities were inhibited. We conclude that adrenal NOS and HO can be induced by a non-lethal dose of endotoxin supporting a modulatory role for these activities in the adrenal response to immune challenges. © 2007 Society for Endocrinology. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220795_v194_n1_p11_Grion |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
bacterium lipopolysaccharide corticotropin endotoxin heme oxygenase inducible nitric oxide synthase n(g) nitroarginine methyl ester neuronal nitric oxide synthase protoporphyrin tin adrenal function animal experiment animal tissue article controlled study enzyme activity enzyme analysis enzyme inhibition genetic code hormone action hormone determination immunomodulation in vivo study male nonhuman priority journal protein expression rat signal transduction steroidogenesis Adrenal Cortex Adrenocorticotropic Hormone Animals Corticosterone Enzyme Activation Enzyme Inhibitors Gene Expression Regulation, Bacterial Heme Oxygenase-1 Lipopolysaccharides Male Metalloporphyrins NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Nitric Oxide Synthase Type II Protoporphyrins Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Stimulation, Chemical |
| spellingShingle |
bacterium lipopolysaccharide corticotropin endotoxin heme oxygenase inducible nitric oxide synthase n(g) nitroarginine methyl ester neuronal nitric oxide synthase protoporphyrin tin adrenal function animal experiment animal tissue article controlled study enzyme activity enzyme analysis enzyme inhibition genetic code hormone action hormone determination immunomodulation in vivo study male nonhuman priority journal protein expression rat signal transduction steroidogenesis Adrenal Cortex Adrenocorticotropic Hormone Animals Corticosterone Enzyme Activation Enzyme Inhibitors Gene Expression Regulation, Bacterial Heme Oxygenase-1 Lipopolysaccharides Male Metalloporphyrins NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Nitric Oxide Synthase Type II Protoporphyrins Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Stimulation, Chemical Grion, N. Repetto, E.M. Pomeraniec, Y. Martinez Calejman, C. Astort, F. Sanchez, R. Pignataro, O.P. Arias, P. Cymeryng, C.B. Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| topic_facet |
bacterium lipopolysaccharide corticotropin endotoxin heme oxygenase inducible nitric oxide synthase n(g) nitroarginine methyl ester neuronal nitric oxide synthase protoporphyrin tin adrenal function animal experiment animal tissue article controlled study enzyme activity enzyme analysis enzyme inhibition genetic code hormone action hormone determination immunomodulation in vivo study male nonhuman priority journal protein expression rat signal transduction steroidogenesis Adrenal Cortex Adrenocorticotropic Hormone Animals Corticosterone Enzyme Activation Enzyme Inhibitors Gene Expression Regulation, Bacterial Heme Oxygenase-1 Lipopolysaccharides Male Metalloporphyrins NG-Nitroarginine Methyl Ester Nitric Oxide Synthase Nitric Oxide Synthase Type II Protoporphyrins Random Allocation Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Stimulation, Chemical |
| description |
The present study was designed to investigate the effect of lipopolysaccharide (LPS) on the expression levels and activities of the nitric oxide synthase (NOS) and heme oxygenase (HO) systems in the rat adrenal gland. Both enzymatic activities were significantly increased in this tissue after in vivo treatment with LPS. The concurrent induction of the HO-1, NOS-1, and NOS-2 gene products was also detected as both mRNAs and protein levels were augmented by this treatment in a time-dependent way. A significant interaction between both signaling systems was also demonstrated as in vivo blockage of NOS activity with N(G)-nitro-L-arginine methyl ester (L-NAME) resulted in a significant reduction in HO expression and activity levels, while an increase in NOS activity was observed when HO was inhibited by Sn-protoporphyrin IX (Sn-PPIX). As both NOS and HO activities have been previously involved in the modulation of adrenal steroidogenesis, we investigated the participation of these signaling systems in the adrenal response to LPS. Our results showed that acute stimulation of steroid production by ACTH was significantly increased when either NOS or HO activities were inhibited. We conclude that adrenal NOS and HO can be induced by a non-lethal dose of endotoxin supporting a modulatory role for these activities in the adrenal response to immune challenges. © 2007 Society for Endocrinology. |
| format |
JOUR |
| author |
Grion, N. Repetto, E.M. Pomeraniec, Y. Martinez Calejman, C. Astort, F. Sanchez, R. Pignataro, O.P. Arias, P. Cymeryng, C.B. |
| author_facet |
Grion, N. Repetto, E.M. Pomeraniec, Y. Martinez Calejman, C. Astort, F. Sanchez, R. Pignataro, O.P. Arias, P. Cymeryng, C.B. |
| author_sort |
Grion, N. |
| title |
Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| title_short |
Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| title_full |
Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| title_fullStr |
Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| title_full_unstemmed |
Induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: Involvement of both signaling systems in the modulation of ACTH-dependent steroid production |
| title_sort |
induction of nitric oxide synthase and heme oxygenase activities by endotoxin in the rat adrenal cortex: involvement of both signaling systems in the modulation of acth-dependent steroid production |
| url |
http://hdl.handle.net/20.500.12110/paper_00220795_v194_n1_p11_Grion |
| work_keys_str_mv |
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1807319775914754048 |