Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration

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The characterization of a biosensor based on poly(allylamine) containing an osmium complex (OsPAA) in a matrix of industrial relevance, like milk, is presented. The biosensor is constructed by crosslinking an oxidase and the redox polymer (OsPAA) with poly(ethylene diglycidyl) ether (PEG). Parameter...

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Detalles Bibliográficos
Autores principales:	Cortón, E., Battaglini, F.
Formato:	JOUR
Materias:	Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Crosslinking Electrochemical electrodes Enzymes Ionic strength Osmium compounds pH effects Polyethers Proteins Redox reactions Thermal effects Buffer solutions
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p1_Corton
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00220728_v511_n1-2_p1_Corton
record_format	dspace
spelling	todo:paper_00220728_v511_n1-2_p1_Corton2023-10-03T14:25:49Z Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration Cortón, E. Battaglini, F. Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzymes Ionic strength Osmium compounds pH effects Polyethers Proteins Redox reactions Thermal effects Buffer solutions Biosensors The characterization of a biosensor based on poly(allylamine) containing an osmium complex (OsPAA) in a matrix of industrial relevance, like milk, is presented. The biosensor is constructed by crosslinking an oxidase and the redox polymer (OsPAA) with poly(ethylene diglycidyl) ether (PEG). Parameters such as the optimum crosslinker concentration, working pH and temperature, and the effect of ionic strength were examined. When the electrode was immersed in milk spiked with substrate, an important decrease in the amperometric signal was observed compared with the one observed in a buffer solution at pH 7.0. The effects of different components of the milk are presented. The decay in the catalytic current is mainly due to the caseins, which insulate the osmium complex from the redox center of the oxidase. © 2001 Elsevier Science B.V. Fil:Cortón, E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Battaglini, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p1_Corton
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzymes Ionic strength Osmium compounds pH effects Polyethers Proteins Redox reactions Thermal effects Buffer solutions Biosensors
spellingShingle	Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzymes Ionic strength Osmium compounds pH effects Polyethers Proteins Redox reactions Thermal effects Buffer solutions Biosensors Cortón, E. Battaglini, F. Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
topic_facet	Biosensors Chronoamperometry Cyclic voltammetry Flow injection analysis Redox enzymes Crosslinking Cyclic voltammetry Electrochemical electrodes Enzymes Ionic strength Osmium compounds pH effects Polyethers Proteins Redox reactions Thermal effects Buffer solutions Biosensors
description	The characterization of a biosensor based on poly(allylamine) containing an osmium complex (OsPAA) in a matrix of industrial relevance, like milk, is presented. The biosensor is constructed by crosslinking an oxidase and the redox polymer (OsPAA) with poly(ethylene diglycidyl) ether (PEG). Parameters such as the optimum crosslinker concentration, working pH and temperature, and the effect of ionic strength were examined. When the electrode was immersed in milk spiked with substrate, an important decrease in the amperometric signal was observed compared with the one observed in a buffer solution at pH 7.0. The effects of different components of the milk are presented. The decay in the catalytic current is mainly due to the caseins, which insulate the osmium complex from the redox center of the oxidase. © 2001 Elsevier Science B.V.
format	JOUR
author	Cortón, E. Battaglini, F.
author_facet	Cortón, E. Battaglini, F.
author_sort	Cortón, E.
title	Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
title_short	Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
title_full	Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
title_fullStr	Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
title_full_unstemmed	Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration
title_sort	effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: part 1. monoenzymatic configuration
url	http://hdl.handle.net/20.500.12110/paper_00220728_v511_n1-2_p1_Corton
work_keys_str_mv	AT cortone effectofmilkproteinsonthebehaviorofabiosensorbasedonpolyallylaminecontaininganosmiumcomplexwiredtoredoxenzymespart1monoenzymaticconfiguration AT battaglinif effectofmilkproteinsonthebehaviorofabiosensorbasedonpolyallylaminecontaininganosmiumcomplexwiredtoredoxenzymespart1monoenzymaticconfiguration
_version_	1807322152637038592

Effect of milk proteins on the behavior of a biosensor based on poly(allylamine) containing an osmium complex wired to redox enzymes: Part 1. Monoenzymatic configuration

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