Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1
Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were i...
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todo:paper_00219541_v231_n7_p1575_Muglia2023-10-03T14:23:39Z Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 Muglia, C.I. Papa Gobbi, R. Smaldini, P. Orsini Delgado, M.L. Candia, M. Zanuzzi, C. Sambuelli, A. Rocca, A. Toscano, M.A. Rabinovich, G.A. Docena, G.H. epidermal growth factor galectin 1 interleukin 10 interleukin 13 interleukin 1beta interleukin 25 interleukin 5 thymic stromal lymphopoietin transforming growth factor beta1 tumor necrosis factor galectin 1 animal cell animal experiment animal model Article cell function cell viability colitis colon controlled study food allergy human human cell in vivo study inflammatory bowel disease intestinal tissue intestine epithelium cell microenvironment mouse nonhuman priority journal protein binding protein function protein secretion small intestine animal genetics inflammation inflammatory bowel disease intestine mucosa metabolism pathology tumor microenvironment Animals Cellular Microenvironment Colitis Colon Food Hypersensitivity Galectin 1 Humans Inflammation Inflammatory Bowel Diseases Intestinal Mucosa Mice Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with different pro-inflammatory cytokines, and Gal-1 binding, secretion of homeostatic factors and viability were assessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 binding and modulation of IECs. We found an enhanced binding of Gal-1 to: (a) murine IECs exposed to IL-1β, TNF, and IL-13; (b) IECs from inflamed areas in intestinal tissue from IBD patients; (c) small bowel of allergic mice; and (d) colon from mice with experimental colitis. Our results showed that low concentrations of Gal-1 favored a tolerogenic micro-environment, whereas high concentrations of this lectin modulated viability of IECs through mechanisms involving activation of caspase-9 and modulation of Bcl-2 protein family members. Our results showed that, when added in the presence of diverse pro-inflammatory cytokines such as tumor necrosis factor (TNF), IL-13 and IL-5, Gal-1 differentially promoted the secretion of growth factors including thymic stromal lymphopoietin (TSLP), epidermal growth factor (EGF), IL-10, IL-25, and transforming growth factor (TGF-β1). In conclusion, we found an augmented binding of Gal-1 to IECs when exposed in vitro or in vivo to inflammatory stimuli, showing different effects depending on Gal-1 concentration. These findings highlight the importance of the inflammatory micro-environment of mucosal tissues in modulating IECs susceptibility to the immunoregulatory lectin Gal-1 and its role in epithelial cell homeostasis. © 2015 Wiley Periodicals, Inc. Fil:Toscano, M.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219541_v231_n7_p1575_Muglia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
epidermal growth factor galectin 1 interleukin 10 interleukin 13 interleukin 1beta interleukin 25 interleukin 5 thymic stromal lymphopoietin transforming growth factor beta1 tumor necrosis factor galectin 1 animal cell animal experiment animal model Article cell function cell viability colitis colon controlled study food allergy human human cell in vivo study inflammatory bowel disease intestinal tissue intestine epithelium cell microenvironment mouse nonhuman priority journal protein binding protein function protein secretion small intestine animal genetics inflammation inflammatory bowel disease intestine mucosa metabolism pathology tumor microenvironment Animals Cellular Microenvironment Colitis Colon Food Hypersensitivity Galectin 1 Humans Inflammation Inflammatory Bowel Diseases Intestinal Mucosa Mice |
| spellingShingle |
epidermal growth factor galectin 1 interleukin 10 interleukin 13 interleukin 1beta interleukin 25 interleukin 5 thymic stromal lymphopoietin transforming growth factor beta1 tumor necrosis factor galectin 1 animal cell animal experiment animal model Article cell function cell viability colitis colon controlled study food allergy human human cell in vivo study inflammatory bowel disease intestinal tissue intestine epithelium cell microenvironment mouse nonhuman priority journal protein binding protein function protein secretion small intestine animal genetics inflammation inflammatory bowel disease intestine mucosa metabolism pathology tumor microenvironment Animals Cellular Microenvironment Colitis Colon Food Hypersensitivity Galectin 1 Humans Inflammation Inflammatory Bowel Diseases Intestinal Mucosa Mice Muglia, C.I. Papa Gobbi, R. Smaldini, P. Orsini Delgado, M.L. Candia, M. Zanuzzi, C. Sambuelli, A. Rocca, A. Toscano, M.A. Rabinovich, G.A. Docena, G.H. Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| topic_facet |
epidermal growth factor galectin 1 interleukin 10 interleukin 13 interleukin 1beta interleukin 25 interleukin 5 thymic stromal lymphopoietin transforming growth factor beta1 tumor necrosis factor galectin 1 animal cell animal experiment animal model Article cell function cell viability colitis colon controlled study food allergy human human cell in vivo study inflammatory bowel disease intestinal tissue intestine epithelium cell microenvironment mouse nonhuman priority journal protein binding protein function protein secretion small intestine animal genetics inflammation inflammatory bowel disease intestine mucosa metabolism pathology tumor microenvironment Animals Cellular Microenvironment Colitis Colon Food Hypersensitivity Galectin 1 Humans Inflammation Inflammatory Bowel Diseases Intestinal Mucosa Mice |
| description |
Galectins play key roles in the inflammatory cascade. In this study, we aimed to analyze the effect of galectin-1 (Gal-1) in the function of intestinal epithelial cells (IECs) isolated from healthy and inflamed mucosa. IECs isolated from mice or patients with inflammatory bowel diseases (IBD) were incubated with different pro-inflammatory cytokines, and Gal-1 binding, secretion of homeostatic factors and viability were assessed. Experimental models of food allergy and colitis were used to evaluate the in vivo influence of inflammation on Gal-1 binding and modulation of IECs. We found an enhanced binding of Gal-1 to: (a) murine IECs exposed to IL-1β, TNF, and IL-13; (b) IECs from inflamed areas in intestinal tissue from IBD patients; (c) small bowel of allergic mice; and (d) colon from mice with experimental colitis. Our results showed that low concentrations of Gal-1 favored a tolerogenic micro-environment, whereas high concentrations of this lectin modulated viability of IECs through mechanisms involving activation of caspase-9 and modulation of Bcl-2 protein family members. Our results showed that, when added in the presence of diverse pro-inflammatory cytokines such as tumor necrosis factor (TNF), IL-13 and IL-5, Gal-1 differentially promoted the secretion of growth factors including thymic stromal lymphopoietin (TSLP), epidermal growth factor (EGF), IL-10, IL-25, and transforming growth factor (TGF-β1). In conclusion, we found an augmented binding of Gal-1 to IECs when exposed in vitro or in vivo to inflammatory stimuli, showing different effects depending on Gal-1 concentration. These findings highlight the importance of the inflammatory micro-environment of mucosal tissues in modulating IECs susceptibility to the immunoregulatory lectin Gal-1 and its role in epithelial cell homeostasis. © 2015 Wiley Periodicals, Inc. |
| format |
JOUR |
| author |
Muglia, C.I. Papa Gobbi, R. Smaldini, P. Orsini Delgado, M.L. Candia, M. Zanuzzi, C. Sambuelli, A. Rocca, A. Toscano, M.A. Rabinovich, G.A. Docena, G.H. |
| author_facet |
Muglia, C.I. Papa Gobbi, R. Smaldini, P. Orsini Delgado, M.L. Candia, M. Zanuzzi, C. Sambuelli, A. Rocca, A. Toscano, M.A. Rabinovich, G.A. Docena, G.H. |
| author_sort |
Muglia, C.I. |
| title |
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| title_short |
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| title_full |
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| title_fullStr |
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| title_full_unstemmed |
Inflammation Controls Sensitivity of Human and Mouse Intestinal Epithelial Cells to Galectin-1 |
| title_sort |
inflammation controls sensitivity of human and mouse intestinal epithelial cells to galectin-1 |
| url |
http://hdl.handle.net/20.500.12110/paper_00219541_v231_n7_p1575_Muglia |
| work_keys_str_mv |
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| _version_ |
1807318873855229952 |