Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4

Bcl-X exists in at least five different isoforms with complex effects on programmed cell death. Glucocorticoids and progestins control bcl-X expression and influence the ratio between bcl-XL (antiapoptotic isoform) and bcl-XS (proapoptotic isoform) in different tissues. The 5′-UTR region of the mous...

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Autores principales:	Viegas, L.R., Vicent, G.P., Barañao, J.L., Beato, M., Pecci, A.
Formato:	JOUR
Materias:	Chemical bonds Correlation methods Cytology Genes RNA Tissue Epithelial cells Mutants Hormones gestagen glucocorticoid protein bcl x steroid hormone alternative RNA splicing animal cell article cell death cell type codon controlled study enhancer region epithelium cell gene activation gene expression regulation gene induction hormone response hormone responsive element nonhuman point mutation priority journal promoter region protein family protein protein interaction tissue specificity translation initiation Animals Apoptosis Base Sequence bcl-X Protein Binding, Competitive Cell Line Cell Nucleus Chromatin Codon COS Cells DNA Exons Gene Expression Regulation Genetic Vectors Glucocorticoids Mice Models, Genetic Molecular Sequence Data Open Reading Frames Plasmids Point Mutation Precipitin Tests Progestins Promoter Regions (Genetics) Protein Binding Protein Biosynthesis Protein Isoforms Proto-Oncogene Proteins c-bcl-2 Receptors, Progesterone Recombinant Proteins RNA, Messenger Steroids Sulfuric Acid Esters Transfection Animalia
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00219258_v279_n11_p9831_Viegas
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00219258_v279_n11_p9831_Viegas2023-10-03T14:23:04Z Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4 Viegas, L.R. Vicent, G.P. Barañao, J.L. Beato, M. Pecci, A. Chemical bonds Correlation methods Cytology Genes RNA Tissue Epithelial cells Mutants Hormones gestagen glucocorticoid protein bcl x steroid hormone alternative RNA splicing animal cell article cell death cell type codon controlled study enhancer region epithelium cell gene activation gene expression regulation gene induction hormone response hormone responsive element nonhuman point mutation priority journal promoter region protein family protein protein interaction tissue specificity translation initiation Animals Apoptosis Base Sequence bcl-X Protein Binding, Competitive Cell Line Cell Nucleus Chromatin Codon COS Cells DNA Exons Gene Expression Regulation Genetic Vectors Glucocorticoids Mice Models, Genetic Molecular Sequence Data Open Reading Frames Plasmids Point Mutation Precipitin Tests Progestins Promoter Regions (Genetics) Protein Binding Protein Biosynthesis Protein Isoforms Proto-Oncogene Proteins c-bcl-2 Receptors, Progesterone Recombinant Proteins RNA RNA, Messenger Steroids Sulfuric Acid Esters Transfection Animalia Bcl-X exists in at least five different isoforms with complex effects on programmed cell death. Glucocorticoids and progestins control bcl-X expression and influence the ratio between bcl-XL (antiapoptotic isoform) and bcl-XS (proapoptotic isoform) in different tissues. The 5′-UTR region of the mouse bcl-X gene contains at least five different promoters, which exhibit a tissue-specific pattern of promoter usage. Several mRNAs with different 5′-leading exons can be generated upon promoter activation. Here we explore the potential of the various bcl-X gene promoters to be regulated by glucocorticoids or progestins. We found that the region located immediately upstream of promoter 4 (P4) contains two hormone response element (HRE)-like sequences at positions -3040 (HRE I) and -3001 (HRE II) relative to the translation initiation codon. These HRE-like sequences confer hormone responsiveness to a core promoter and bind glucocorticoid or progesterone receptors in vitro. Point mutations of both HREs that prevent steroid receptor binding also eliminate hormonal inducibility. In cells treated with glucocorticoids, the hormone receptor is recruited to the P4 region containing the HREs. Analysis of the products of the endogenous bcl-X in epithelial mammary cells showed that only transcripts originating from P4 increased upon hormone treatment. This observation correlates with the induction of the bcl-XL mRNA, suggesting that P4 is one of the bcl-X promoters responsible for the generation of this antiapoptotic isoform. Fil:Vicent, G.P. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Barañao, J.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pecci, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219258_v279_n11_p9831_Viegas
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Chemical bonds Correlation methods Cytology Genes RNA Tissue Epithelial cells Mutants Hormones gestagen glucocorticoid protein bcl x steroid hormone alternative RNA splicing animal cell article cell death cell type codon controlled study enhancer region epithelium cell gene activation gene expression regulation gene induction hormone response hormone responsive element nonhuman point mutation priority journal promoter region protein family protein protein interaction tissue specificity translation initiation Animals Apoptosis Base Sequence bcl-X Protein Binding, Competitive Cell Line Cell Nucleus Chromatin Codon COS Cells DNA Exons Gene Expression Regulation Genetic Vectors Glucocorticoids Mice Models, Genetic Molecular Sequence Data Open Reading Frames Plasmids Point Mutation Precipitin Tests Progestins Promoter Regions (Genetics) Protein Binding Protein Biosynthesis Protein Isoforms Proto-Oncogene Proteins c-bcl-2 Receptors, Progesterone Recombinant Proteins RNA RNA, Messenger Steroids Sulfuric Acid Esters Transfection Animalia
spellingShingle	Chemical bonds Correlation methods Cytology Genes RNA Tissue Epithelial cells Mutants Hormones gestagen glucocorticoid protein bcl x steroid hormone alternative RNA splicing animal cell article cell death cell type codon controlled study enhancer region epithelium cell gene activation gene expression regulation gene induction hormone response hormone responsive element nonhuman point mutation priority journal promoter region protein family protein protein interaction tissue specificity translation initiation Animals Apoptosis Base Sequence bcl-X Protein Binding, Competitive Cell Line Cell Nucleus Chromatin Codon COS Cells DNA Exons Gene Expression Regulation Genetic Vectors Glucocorticoids Mice Models, Genetic Molecular Sequence Data Open Reading Frames Plasmids Point Mutation Precipitin Tests Progestins Promoter Regions (Genetics) Protein Binding Protein Biosynthesis Protein Isoforms Proto-Oncogene Proteins c-bcl-2 Receptors, Progesterone Recombinant Proteins RNA RNA, Messenger Steroids Sulfuric Acid Esters Transfection Animalia Viegas, L.R. Vicent, G.P. Barañao, J.L. Beato, M. Pecci, A. Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
topic_facet	Chemical bonds Correlation methods Cytology Genes RNA Tissue Epithelial cells Mutants Hormones gestagen glucocorticoid protein bcl x steroid hormone alternative RNA splicing animal cell article cell death cell type codon controlled study enhancer region epithelium cell gene activation gene expression regulation gene induction hormone response hormone responsive element nonhuman point mutation priority journal promoter region protein family protein protein interaction tissue specificity translation initiation Animals Apoptosis Base Sequence bcl-X Protein Binding, Competitive Cell Line Cell Nucleus Chromatin Codon COS Cells DNA Exons Gene Expression Regulation Genetic Vectors Glucocorticoids Mice Models, Genetic Molecular Sequence Data Open Reading Frames Plasmids Point Mutation Precipitin Tests Progestins Promoter Regions (Genetics) Protein Binding Protein Biosynthesis Protein Isoforms Proto-Oncogene Proteins c-bcl-2 Receptors, Progesterone Recombinant Proteins RNA RNA, Messenger Steroids Sulfuric Acid Esters Transfection Animalia
description	Bcl-X exists in at least five different isoforms with complex effects on programmed cell death. Glucocorticoids and progestins control bcl-X expression and influence the ratio between bcl-XL (antiapoptotic isoform) and bcl-XS (proapoptotic isoform) in different tissues. The 5′-UTR region of the mouse bcl-X gene contains at least five different promoters, which exhibit a tissue-specific pattern of promoter usage. Several mRNAs with different 5′-leading exons can be generated upon promoter activation. Here we explore the potential of the various bcl-X gene promoters to be regulated by glucocorticoids or progestins. We found that the region located immediately upstream of promoter 4 (P4) contains two hormone response element (HRE)-like sequences at positions -3040 (HRE I) and -3001 (HRE II) relative to the translation initiation codon. These HRE-like sequences confer hormone responsiveness to a core promoter and bind glucocorticoid or progesterone receptors in vitro. Point mutations of both HREs that prevent steroid receptor binding also eliminate hormonal inducibility. In cells treated with glucocorticoids, the hormone receptor is recruited to the P4 region containing the HREs. Analysis of the products of the endogenous bcl-X in epithelial mammary cells showed that only transcripts originating from P4 increased upon hormone treatment. This observation correlates with the induction of the bcl-XL mRNA, suggesting that P4 is one of the bcl-X promoters responsible for the generation of this antiapoptotic isoform.
format	JOUR
author	Viegas, L.R. Vicent, G.P. Barañao, J.L. Beato, M. Pecci, A.
author_facet	Viegas, L.R. Vicent, G.P. Barañao, J.L. Beato, M. Pecci, A.
author_sort	Viegas, L.R.
title	Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
title_short	Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
title_full	Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
title_fullStr	Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
title_full_unstemmed	Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4
title_sort	steroid hormones induce bcl-x gene expression through direct activation of distal promoter p4
url	http://hdl.handle.net/20.500.12110/paper_00219258_v279_n11_p9831_Viegas
work_keys_str_mv	AT viegaslr steroidhormonesinducebclxgeneexpressionthroughdirectactivationofdistalpromoterp4 AT vicentgp steroidhormonesinducebclxgeneexpressionthroughdirectactivationofdistalpromoterp4 AT baranaojl steroidhormonesinducebclxgeneexpressionthroughdirectactivationofdistalpromoterp4 AT beatom steroidhormonesinducebclxgeneexpressionthroughdirectactivationofdistalpromoterp4 AT peccia steroidhormonesinducebclxgeneexpressionthroughdirectactivationofdistalpromoterp4
_version_	1782025489971412992

Steroid Hormones Induce bcl-X Gene Expression through Direct Activation of Distal Promoter P4

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