Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M....

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Autores principales:	De Lederkremer, R.M., Lima, C., Ramirez, M.I., Ferguson, M.A.J., Homans, S.W., Thomas-Oates, J.
Formato:	JOUR
Materias:	glycan glycan phosphate lipoprotein unclassified drug article chemical structure epimastigote nonhuman priority journal trypanosoma cruzi Acylation Animal Carbohydrate Sequence Chromatography, High Pressure Liquid Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides Peptidoglycan Phospholipids Spectrometry, Mass, Fast Atom Bombardment Support, Non-U.S. Gov't Trypanosoma cruzi
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00219258_v266_n35_p23670_DeLederkremer
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00219258_v266_n35_p23670_DeLederkremer2023-10-03T14:22:56Z Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes De Lederkremer, R.M. Lima, C. Ramirez, M.I. Ferguson, M.A.J. Homans, S.W. Thomas-Oates, J. glycan glycan phosphate lipoprotein unclassified drug article chemical structure epimastigote nonhuman priority journal trypanosoma cruzi Acylation Animal Carbohydrate Sequence Chromatography, High Pressure Liquid Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides Peptidoglycan Phospholipids Spectrometry, Mass, Fast Atom Bombardment Support, Non-U.S. Gov't Trypanosoma cruzi The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00219258_v266_n35_p23670_DeLederkremer
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	glycan glycan phosphate lipoprotein unclassified drug article chemical structure epimastigote nonhuman priority journal trypanosoma cruzi Acylation Animal Carbohydrate Sequence Chromatography, High Pressure Liquid Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides Peptidoglycan Phospholipids Spectrometry, Mass, Fast Atom Bombardment Support, Non-U.S. Gov't Trypanosoma cruzi
spellingShingle	glycan glycan phosphate lipoprotein unclassified drug article chemical structure epimastigote nonhuman priority journal trypanosoma cruzi Acylation Animal Carbohydrate Sequence Chromatography, High Pressure Liquid Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides Peptidoglycan Phospholipids Spectrometry, Mass, Fast Atom Bombardment Support, Non-U.S. Gov't Trypanosoma cruzi De Lederkremer, R.M. Lima, C. Ramirez, M.I. Ferguson, M.A.J. Homans, S.W. Thomas-Oates, J. Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
topic_facet	glycan glycan phosphate lipoprotein unclassified drug article chemical structure epimastigote nonhuman priority journal trypanosoma cruzi Acylation Animal Carbohydrate Sequence Chromatography, High Pressure Liquid Magnetic Resonance Spectroscopy Molecular Sequence Data Oligosaccharides Peptidoglycan Phospholipids Spectrometry, Mass, Fast Atom Bombardment Support, Non-U.S. Gov't Trypanosoma cruzi
description	The lipopeptidophosphoglycan is the major cell surface glycoconjugate of the epimastigote forms of the parasitic protozoan Trypanosoma cruzi. A detailed partial structure for this molecule has been reported (Previato, J. O., Gorin, P. A. J., Mazurek, M., Xavier, M. T., Fournet, B., Wieruszesk, J. M., and Mendonca-Previato, L. (1990) J. Biol. Chem. 265, 2518-2526). In this study, we complete the primary structure assignments and describe the microheterogeneity found in the lipopeptidophosphoglycan glycan, using a combination of 1H and 31P NMR, fast atom bombardment mass spectrometry, methylation linkage analysis, and exoglycosidase sequencing. The lipopeptidophosphoglycan is a glycosylated inositol-phosphoceramide with striking homology to glycosylphosphatidylinositol membrane anchors found attached to a wide variety of plasma membrane proteins throughout the eukaryotes.
format	JOUR
author	De Lederkremer, R.M. Lima, C. Ramirez, M.I. Ferguson, M.A.J. Homans, S.W. Thomas-Oates, J.
author_facet	De Lederkremer, R.M. Lima, C. Ramirez, M.I. Ferguson, M.A.J. Homans, S.W. Thomas-Oates, J.
author_sort	De Lederkremer, R.M.
title	Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
title_short	Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
title_full	Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
title_fullStr	Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
title_full_unstemmed	Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes
title_sort	complete structure of the glycan of lipopeptidophosphoglycan from trypanosoma cruzi epimastigotes
url	http://hdl.handle.net/20.500.12110/paper_00219258_v266_n35_p23670_DeLederkremer
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Complete structure of the glycan of lipopeptidophosphoglycan from Trypanosoma cruzi epimastigotes

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