Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus
Conventional histochemistry and the binding patterns of 22 biotinylated lectins were examined for characterisation of glycoconjugates in the components of the olfactory mucosa of the armadillo Chaetophractus villosus. The mucous lining the olfactory epithelium showed binding sites for DSL, WGA, STL,...
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todo:paper_00218782_v194_n3_p395_Ferrari2023-10-03T14:21:39Z Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus Ferrari, C.C. Carmanchahi, P.D. Aldana Marcos, H.J. Mugnaini, M.T. Affanni, J.M. Paz, D.A. Bowman's glands Histochemistry Lectins Olfactory epithelium Xenarthra concanavalin a glycoconjugate lectin wheat germ agglutinin animal tissue armadillo article basal cell controlled study female histochemistry lamina propria male nonhuman olfactory epithelium olfactory receptor priority journal Acetylglucosamine Animals Armadillos Female Fucose Galactose Glycoconjugates Histocytochemistry Lectins Male Mannose Olfactory Mucosa Conventional histochemistry and the binding patterns of 22 biotinylated lectins were examined for characterisation of glycoconjugates in the components of the olfactory mucosa of the armadillo Chaetophractus villosus. The mucous lining the olfactory epithelium showed binding sites for DSL, WGA, STL, LEL, PHA-E and JAC. Only the basilar processes of the supporting cells stained for Con-A and S-Con A. The olfactory receptor neurons stained with LEL, LCA, Con A, S-Con A, JAC and PNA. The layer of basal cells did not react with any of the lectins studied. Bowman's glands in the lamina propria showed subpopulations of acinar cells reacting with SBA, S-WGA, WGA, STL, Con A, PSA, PNA, SJA, VVA, JAC and S-Con A, but in our optical studies with lectins we were unable to differentiate between mucous and serous cells in the way that is possible on electron microscopy. The ducts of Bowman's glands were labelled with S-WGA, STL, LEL, PHA-E, BSL-I and JAC. This histochemical study on the glycoconjugates of the olfactory mucosa in the order Xenarthra provides a basis for further experimental investigations. Fil:Ferrari, C.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Carmanchahi, P.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Aldana Marcos, H.J. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00218782_v194_n3_p395_Ferrari |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Bowman's glands Histochemistry Lectins Olfactory epithelium Xenarthra concanavalin a glycoconjugate lectin wheat germ agglutinin animal tissue armadillo article basal cell controlled study female histochemistry lamina propria male nonhuman olfactory epithelium olfactory receptor priority journal Acetylglucosamine Animals Armadillos Female Fucose Galactose Glycoconjugates Histocytochemistry Lectins Male Mannose Olfactory Mucosa |
| spellingShingle |
Bowman's glands Histochemistry Lectins Olfactory epithelium Xenarthra concanavalin a glycoconjugate lectin wheat germ agglutinin animal tissue armadillo article basal cell controlled study female histochemistry lamina propria male nonhuman olfactory epithelium olfactory receptor priority journal Acetylglucosamine Animals Armadillos Female Fucose Galactose Glycoconjugates Histocytochemistry Lectins Male Mannose Olfactory Mucosa Ferrari, C.C. Carmanchahi, P.D. Aldana Marcos, H.J. Mugnaini, M.T. Affanni, J.M. Paz, D.A. Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| topic_facet |
Bowman's glands Histochemistry Lectins Olfactory epithelium Xenarthra concanavalin a glycoconjugate lectin wheat germ agglutinin animal tissue armadillo article basal cell controlled study female histochemistry lamina propria male nonhuman olfactory epithelium olfactory receptor priority journal Acetylglucosamine Animals Armadillos Female Fucose Galactose Glycoconjugates Histocytochemistry Lectins Male Mannose Olfactory Mucosa |
| description |
Conventional histochemistry and the binding patterns of 22 biotinylated lectins were examined for characterisation of glycoconjugates in the components of the olfactory mucosa of the armadillo Chaetophractus villosus. The mucous lining the olfactory epithelium showed binding sites for DSL, WGA, STL, LEL, PHA-E and JAC. Only the basilar processes of the supporting cells stained for Con-A and S-Con A. The olfactory receptor neurons stained with LEL, LCA, Con A, S-Con A, JAC and PNA. The layer of basal cells did not react with any of the lectins studied. Bowman's glands in the lamina propria showed subpopulations of acinar cells reacting with SBA, S-WGA, WGA, STL, Con A, PSA, PNA, SJA, VVA, JAC and S-Con A, but in our optical studies with lectins we were unable to differentiate between mucous and serous cells in the way that is possible on electron microscopy. The ducts of Bowman's glands were labelled with S-WGA, STL, LEL, PHA-E, BSL-I and JAC. This histochemical study on the glycoconjugates of the olfactory mucosa in the order Xenarthra provides a basis for further experimental investigations. |
| format |
JOUR |
| author |
Ferrari, C.C. Carmanchahi, P.D. Aldana Marcos, H.J. Mugnaini, M.T. Affanni, J.M. Paz, D.A. |
| author_facet |
Ferrari, C.C. Carmanchahi, P.D. Aldana Marcos, H.J. Mugnaini, M.T. Affanni, J.M. Paz, D.A. |
| author_sort |
Ferrari, C.C. |
| title |
Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| title_short |
Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| title_full |
Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| title_fullStr |
Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| title_full_unstemmed |
Identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo Chaetophractus villosus |
| title_sort |
identification and localisation of glycoconjugates in the olfactory mucosa of the armadillo chaetophractus villosus |
| url |
http://hdl.handle.net/20.500.12110/paper_00218782_v194_n3_p395_Ferrari |
| work_keys_str_mv |
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1807314643829391360 |