Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies

Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands...

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Detalles Bibliográficos
Autores principales:	Belov, V.N., Bossi, M.L.
Formato:	JOUR
Materias:	fluorescence optical nanoscopy photochemistry photochromism
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00212148_v53_n5_p267_Belov
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spelling	todo:paper_00212148_v53_n5_p267_Belov2023-10-03T14:20:46Z Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies Belov, V.N. Bossi, M.L. fluorescence optical nanoscopy photochemistry photochromism Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim. Fil:Bossi, M.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	fluorescence optical nanoscopy photochemistry photochromism
spellingShingle	fluorescence optical nanoscopy photochemistry photochromism Belov, V.N. Bossi, M.L. Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
topic_facet	fluorescence optical nanoscopy photochemistry photochromism
description	Spatial resolution in far-field fluorescence microscopy is limited by diffraction to about 200 nm. With the aid of photoswitchable fluorophores, the diffraction barrier has been successfully overcome, allowing unprecedented resolution in the order of single biomolecules. The imaging process demands markers with strict and reliable control of the switching, to keep most of the markers in a non-emissive state most of the time and to bring a tiny number back to an emissive state, and detection at the single-molecule level. Herein, we describe the use of rhodamine spiroamides with unique photophysical properties as molecular probes for super-resolution techniques based on the localization of single emitters. This family of photochromic and fluorescent compounds fulfils the stringent requirements for such imaging methods; these compounds are robust and capable of enduring single-molecule detection in diverse environments. This has allowed meaningful images with resolution down to a few nanometres. Their design, synthesis and implementation is discussed along with imaging applications in material and life sciences. Copyright © 2013 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.
format	JOUR
author	Belov, V.N. Bossi, M.L.
author_facet	Belov, V.N. Bossi, M.L.
author_sort	Belov, V.N.
title	Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_short	Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_full	Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_fullStr	Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_full_unstemmed	Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
title_sort	photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies
url	http://hdl.handle.net/20.500.12110/paper_00212148_v53_n5_p267_Belov
work_keys_str_mv	AT belovvn photoswitchingemissionwithrhodaminespiroamidesforsuperresolutionfluorescencenanoscopies AT bossiml photoswitchingemissionwithrhodaminespiroamidesforsuperresolutionfluorescencenanoscopies
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Photoswitching emission with rhodamine spiroamides for super-resolution fluorescence nanoscopies

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