Anti-tumor activity of cytokines against opportunistic vascular tumors in mice
Polyoma middle T (PmT)-transformed endothelial cells may represent a unique murine model for human opportunistic vascular tumors. The present study was designed to evaluate the anti-tumor potential of a panel of 13 cytokines against murine PmT-transformed endothelial cells. Interferon gamma (IFNγ) a...
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todo:paper_00207136_v65_n5_p700_Dong2023-10-03T14:18:25Z Anti-tumor activity of cytokines against opportunistic vascular tumors in mice Dong, Q.G. Graziani, A. Garlanda, C. Wainstok De Calmanovici, R. Arese, M. Soldi, R. Vecchi, A. Mantovani, A. Bussolino, F. alpha interferon beta interferon gamma interferon granulocyte macrophage colony stimulating factor interleukin 1 interleukin 13 interleukin 2 interleukin 4 interleukin 6 leukemia inhibitory factor oncostatin m transforming growth factor beta tumor necrosis factor alpha animal cell animal model article cell transformation controlled study endothelium cell growth inhibition mouse priority journal vascular tumor 1-Phosphatidylinositol 3-Kinase Animals Antigens, Polyomavirus Transforming Cell Division Cell Transformation, Viral Cytokines Female Hemangioendothelioma Mice Mice, Inbred C57BL Mice, Nude Phosphatidylinositols Phosphorylation Phosphotransferases (Alcohol Group Acceptor) Tumor Cells, Cultured Vascular Neoplasms Polyoma middle T (PmT)-transformed endothelial cells may represent a unique murine model for human opportunistic vascular tumors. The present study was designed to evaluate the anti-tumor potential of a panel of 13 cytokines against murine PmT-transformed endothelial cells. Interferon gamma (IFNγ) and transforming growth factor beta 1 (TGFβ1) substantially decreased in a dose-dependent manner the proliferation of a panel of 6 PmT-transformed cell lines. IFNα and tumor necrosis factor α (TNFα) had marginal anti-proliferative activity, whereas other molecules (interleukins-1, -2, -4, -6 and -13, IFNβ, leukemia inhibitory factor, oncostatin M, granulocyte-macrophage colony-stimulating factor) caused no growth inhibition. IFNγ and TGFβ1 were therefore selected for further analysis of their mechanism of action and in vivo relevance. IFNγ and TGFβ1 reduced the activity of phosphatidylinositol-3-kinase and the production of phosphatidylinositol 3,4-biphosphate, without modifying the tyrosine kinase(s) activity associated with PmT. IFNγ and TGFβ1 were also tested for their ability to modify the in vivo growth of the PmT-transformed endothelial cells H5V in syngeneic C57B1/6 mice. Treatment with IFNα and TGFβ1 significantly delayed tumor growth and increased survival time. In contrast, treatment with IFNα and TNFα failed to prolong survival. In nude mice, IFNα and TGFβ1 1 had a transient effect on tumor growth but no effect on survival, suggesting a contribution of T cells to the in vivo anti-tumor activity of these cytokines. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00207136_v65_n5_p700_Dong |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
alpha interferon beta interferon gamma interferon granulocyte macrophage colony stimulating factor interleukin 1 interleukin 13 interleukin 2 interleukin 4 interleukin 6 leukemia inhibitory factor oncostatin m transforming growth factor beta tumor necrosis factor alpha animal cell animal model article cell transformation controlled study endothelium cell growth inhibition mouse priority journal vascular tumor 1-Phosphatidylinositol 3-Kinase Animals Antigens, Polyomavirus Transforming Cell Division Cell Transformation, Viral Cytokines Female Hemangioendothelioma Mice Mice, Inbred C57BL Mice, Nude Phosphatidylinositols Phosphorylation Phosphotransferases (Alcohol Group Acceptor) Tumor Cells, Cultured Vascular Neoplasms |
| spellingShingle |
alpha interferon beta interferon gamma interferon granulocyte macrophage colony stimulating factor interleukin 1 interleukin 13 interleukin 2 interleukin 4 interleukin 6 leukemia inhibitory factor oncostatin m transforming growth factor beta tumor necrosis factor alpha animal cell animal model article cell transformation controlled study endothelium cell growth inhibition mouse priority journal vascular tumor 1-Phosphatidylinositol 3-Kinase Animals Antigens, Polyomavirus Transforming Cell Division Cell Transformation, Viral Cytokines Female Hemangioendothelioma Mice Mice, Inbred C57BL Mice, Nude Phosphatidylinositols Phosphorylation Phosphotransferases (Alcohol Group Acceptor) Tumor Cells, Cultured Vascular Neoplasms Dong, Q.G. Graziani, A. Garlanda, C. Wainstok De Calmanovici, R. Arese, M. Soldi, R. Vecchi, A. Mantovani, A. Bussolino, F. Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| topic_facet |
alpha interferon beta interferon gamma interferon granulocyte macrophage colony stimulating factor interleukin 1 interleukin 13 interleukin 2 interleukin 4 interleukin 6 leukemia inhibitory factor oncostatin m transforming growth factor beta tumor necrosis factor alpha animal cell animal model article cell transformation controlled study endothelium cell growth inhibition mouse priority journal vascular tumor 1-Phosphatidylinositol 3-Kinase Animals Antigens, Polyomavirus Transforming Cell Division Cell Transformation, Viral Cytokines Female Hemangioendothelioma Mice Mice, Inbred C57BL Mice, Nude Phosphatidylinositols Phosphorylation Phosphotransferases (Alcohol Group Acceptor) Tumor Cells, Cultured Vascular Neoplasms |
| description |
Polyoma middle T (PmT)-transformed endothelial cells may represent a unique murine model for human opportunistic vascular tumors. The present study was designed to evaluate the anti-tumor potential of a panel of 13 cytokines against murine PmT-transformed endothelial cells. Interferon gamma (IFNγ) and transforming growth factor beta 1 (TGFβ1) substantially decreased in a dose-dependent manner the proliferation of a panel of 6 PmT-transformed cell lines. IFNα and tumor necrosis factor α (TNFα) had marginal anti-proliferative activity, whereas other molecules (interleukins-1, -2, -4, -6 and -13, IFNβ, leukemia inhibitory factor, oncostatin M, granulocyte-macrophage colony-stimulating factor) caused no growth inhibition. IFNγ and TGFβ1 were therefore selected for further analysis of their mechanism of action and in vivo relevance. IFNγ and TGFβ1 reduced the activity of phosphatidylinositol-3-kinase and the production of phosphatidylinositol 3,4-biphosphate, without modifying the tyrosine kinase(s) activity associated with PmT. IFNγ and TGFβ1 were also tested for their ability to modify the in vivo growth of the PmT-transformed endothelial cells H5V in syngeneic C57B1/6 mice. Treatment with IFNα and TGFβ1 significantly delayed tumor growth and increased survival time. In contrast, treatment with IFNα and TNFα failed to prolong survival. In nude mice, IFNα and TGFβ1 1 had a transient effect on tumor growth but no effect on survival, suggesting a contribution of T cells to the in vivo anti-tumor activity of these cytokines. |
| format |
JOUR |
| author |
Dong, Q.G. Graziani, A. Garlanda, C. Wainstok De Calmanovici, R. Arese, M. Soldi, R. Vecchi, A. Mantovani, A. Bussolino, F. |
| author_facet |
Dong, Q.G. Graziani, A. Garlanda, C. Wainstok De Calmanovici, R. Arese, M. Soldi, R. Vecchi, A. Mantovani, A. Bussolino, F. |
| author_sort |
Dong, Q.G. |
| title |
Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| title_short |
Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| title_full |
Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| title_fullStr |
Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| title_full_unstemmed |
Anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| title_sort |
anti-tumor activity of cytokines against opportunistic vascular tumors in mice |
| url |
http://hdl.handle.net/20.500.12110/paper_00207136_v65_n5_p700_Dong |
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