A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria

1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also con...

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Detalles Bibliográficos
Autores principales: Del C. Batlle, A.M., De Xifra, E.A.W., MarÍa Stella, A.
Formato: JOUR
Materias:
erythrocyte enzyme
acute intermittent porphyria
blood and hemopoietic system
diagnosis
methodology
porphobilinogenase
Aminolevulinic Acid
Ammonia-Lyases
Comparative Study
Erythrocytes
Hemolysis
Human
Hydrogen-Ion Concentration
Porphobilinogen
Porphyria
Spectrophotometry
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also containing porphobilinogen, NaCl. MgCl2. Tris-HCl buffer pH 8.2; which is incubated aerobically, in the dark. at 45°. for 1 hr with shaking. 3. 3. The enzymic activity is quenched by adding TCA. 4. 4. Porphyrinogens are oxidized, protein precipitated centrifuged off and uroporphyrins formed are determined spectrophotometrically in the supernatant. 5. 5. The enzyme is stable up to 1 month if stored at -20°. © 1978.

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