A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria
1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also con...
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todo:paper_0020711X_v9_n12_p871_DelCBatlle2023-10-03T14:18:14Z A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria Del C. Batlle, A.M. De Xifra, E.A.W. MarÍa Stella, A. erythrocyte enzyme acute intermittent porphyria blood and hemopoietic system diagnosis methodology porphobilinogenase Aminolevulinic Acid Ammonia-Lyases Comparative Study Erythrocytes Hemolysis Human Hydrogen-Ion Concentration Porphobilinogen Porphyria Spectrophotometry 1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also containing porphobilinogen, NaCl. MgCl2. Tris-HCl buffer pH 8.2; which is incubated aerobically, in the dark. at 45°. for 1 hr with shaking. 3. 3. The enzymic activity is quenched by adding TCA. 4. 4. Porphyrinogens are oxidized, protein precipitated centrifuged off and uroporphyrins formed are determined spectrophotometrically in the supernatant. 5. 5. The enzyme is stable up to 1 month if stored at -20°. © 1978. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
erythrocyte enzyme acute intermittent porphyria blood and hemopoietic system diagnosis methodology porphobilinogenase Aminolevulinic Acid Ammonia-Lyases Comparative Study Erythrocytes Hemolysis Human Hydrogen-Ion Concentration Porphobilinogen Porphyria Spectrophotometry |
| spellingShingle |
erythrocyte enzyme acute intermittent porphyria blood and hemopoietic system diagnosis methodology porphobilinogenase Aminolevulinic Acid Ammonia-Lyases Comparative Study Erythrocytes Hemolysis Human Hydrogen-Ion Concentration Porphobilinogen Porphyria Spectrophotometry Del C. Batlle, A.M. De Xifra, E.A.W. MarÍa Stella, A. A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| topic_facet |
erythrocyte enzyme acute intermittent porphyria blood and hemopoietic system diagnosis methodology porphobilinogenase Aminolevulinic Acid Ammonia-Lyases Comparative Study Erythrocytes Hemolysis Human Hydrogen-Ion Concentration Porphobilinogen Porphyria Spectrophotometry |
| description |
1. l. A simple method for measuring erythrocyte porphobilinogenase activity has been devised. 2. 2. Red blood cells are hemolysed by three freeze-thaws, the hemolysate is diluted 15 times and only 0.5 ml of this preparation is used for determining porphobilinogenase. in an incubation system also containing porphobilinogen, NaCl. MgCl2. Tris-HCl buffer pH 8.2; which is incubated aerobically, in the dark. at 45°. for 1 hr with shaking. 3. 3. The enzymic activity is quenched by adding TCA. 4. 4. Porphyrinogens are oxidized, protein precipitated centrifuged off and uroporphyrins formed are determined spectrophotometrically in the supernatant. 5. 5. The enzyme is stable up to 1 month if stored at -20°. © 1978. |
| format |
JOUR |
| author |
Del C. Batlle, A.M. De Xifra, E.A.W. MarÍa Stella, A. |
| author_facet |
Del C. Batlle, A.M. De Xifra, E.A.W. MarÍa Stella, A. |
| author_sort |
Del C. Batlle, A.M. |
| title |
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| title_short |
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| title_full |
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| title_fullStr |
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| title_full_unstemmed |
A simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| title_sort |
simple method for measuring erythrocyte porphobilinogenase, and its use in the diagnosis of acute intermittent porphyria |
| url |
http://hdl.handle.net/20.500.12110/paper_0020711X_v9_n12_p871_DelCBatlle |
| work_keys_str_mv |
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| _version_ |
1782024105832218624 |