Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions

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1. 1. Effects of various factors on chlorophyll, porphyrin and protein content, growth and on the activities of the enzymes involved in the earlier stages of tetrapyrrole synthesis, in cultured soybean cells, were studied. 2. 2. When dark-grown callus was exposed to light, it was found that the amou...

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Autores principales: Del C. Batlle, A.M., Llambias, E.B.C., de Xlfra, E.W., Tigier, H.A.
Formato: JOUR
Materias:
Aminolevulic Acid Dehydratase
Aminolevulic Acid Synthetase
chlorophyll
Coproporphyrinogenase
Decarboxylase
growth
Porphobilinogenase
Porphyrin biosynthesis
regulation
soybean callus tissue
succinyl CoA synthetase
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v6_n8_p591_DelCBatlle
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
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spelling todo:paper_0020711X_v6_n8_p591_DelCBatlle2023-10-03T14:18:10Z Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions Del C. Batlle, A.M. Llambias, E.B.C. de Xlfra, E.W. Tigier, H.A. Aminolevulic Acid Dehydratase Aminolevulic Acid Synthetase chlorophyll Coproporphyrinogenase Decarboxylase growth Porphobilinogenase Porphyrin biosynthesis regulation soybean callus tissue succinyl CoA synthetase 1. 1. Effects of various factors on chlorophyll, porphyrin and protein content, growth and on the activities of the enzymes involved in the earlier stages of tetrapyrrole synthesis, in cultured soybean cells, were studied. 2. 2. When dark-grown callus was exposed to light, it was found that the amount of porphyrins formed was not altered, but chlorophyll content as well as Succinyl CoA Synthetase (Suc.CoA-S), Aminolevulic Acid Synthetase (ALA-S), Aminolevulic Acid Dehydratase (ALA-D) and Porphobilinogenase (PBGase) activities increased. 3. 3. Addition of Aminolevulic Acid (ALA) to the medium culture, was found to stimulate porphyrin accumulation and to prevent growth; however chlorophyll content was not significantly modified. ALA-S was inhibited while both ALA-D and PBGase activities were enhanced. The action of puromycin and mitomycin added along with ALA to the media, was also studied, but neither of these inhibitors modified much the effects produced by ALA. 4. 4. Addition of Porphobilinogen (PBG), showed accumulation of uroporphyrin in the tissue; except inhibition of ALA-S, enzymes activities, protein and chlorophyll content were not modified. Evidence obtained would indicate that callus tissue was not permeable to PBG. 5. 5. Omission of iron from the culture medium, produced porphyrin accumulation and prevented growth. It has been consistently found that, the higher the content of porphyrins, the less the callus growth. Coproporphyrin was the major component of the porphyrins formed in ALA supplemented or iron deficient media. ALA-S and ALA-D were reduced under iron deficiency. 6. 6. The addition of ATP to the media, did not affect porphyrin, protein, and chlorophyll synthesis, growth or ALA-D, but Suc.CoA-S, ALA-S and PBGase activities diminished. 7. 7. Gibberelic acid produced a measurable increase of PBGase, while diminished Suc.CoA-S and ALA-S. 8. 8. Succinate increased growth and inhibited ALA-S and ALA-D. 9. 9. Carbonyl cyanide m-chlorophenyl hydrazine (CCCP), added to the medium produced accumulation of porphyrins, consequently, ALA-S was greatly inhibited and growth prevented. PBGase was also diminished. 10. 10. Coproporphyrinogenase and Decarboxylases activities were hardly detected in most experiments, and are limiting. 11. 11. The complex pattern of results obtained is discussed. © 1975. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Llambias, E.B.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Tigier, H.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v6_n8_p591_DelCBatlle
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Aminolevulic Acid Dehydratase
Aminolevulic Acid Synthetase
chlorophyll
Coproporphyrinogenase
Decarboxylase
growth
Porphobilinogenase
Porphyrin biosynthesis
regulation
soybean callus tissue
succinyl CoA synthetase
spellingShingle Aminolevulic Acid Dehydratase
Aminolevulic Acid Synthetase
chlorophyll
Coproporphyrinogenase
Decarboxylase
growth
Porphobilinogenase
Porphyrin biosynthesis
regulation
soybean callus tissue
succinyl CoA synthetase
Del C. Batlle, A.M.
Llambias, E.B.C.
de Xlfra, E.W.
Tigier, H.A.
Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
topic_facet Aminolevulic Acid Dehydratase
Aminolevulic Acid Synthetase
chlorophyll
Coproporphyrinogenase
Decarboxylase
growth
Porphobilinogenase
Porphyrin biosynthesis
regulation
soybean callus tissue
succinyl CoA synthetase
description 1. 1. Effects of various factors on chlorophyll, porphyrin and protein content, growth and on the activities of the enzymes involved in the earlier stages of tetrapyrrole synthesis, in cultured soybean cells, were studied. 2. 2. When dark-grown callus was exposed to light, it was found that the amount of porphyrins formed was not altered, but chlorophyll content as well as Succinyl CoA Synthetase (Suc.CoA-S), Aminolevulic Acid Synthetase (ALA-S), Aminolevulic Acid Dehydratase (ALA-D) and Porphobilinogenase (PBGase) activities increased. 3. 3. Addition of Aminolevulic Acid (ALA) to the medium culture, was found to stimulate porphyrin accumulation and to prevent growth; however chlorophyll content was not significantly modified. ALA-S was inhibited while both ALA-D and PBGase activities were enhanced. The action of puromycin and mitomycin added along with ALA to the media, was also studied, but neither of these inhibitors modified much the effects produced by ALA. 4. 4. Addition of Porphobilinogen (PBG), showed accumulation of uroporphyrin in the tissue; except inhibition of ALA-S, enzymes activities, protein and chlorophyll content were not modified. Evidence obtained would indicate that callus tissue was not permeable to PBG. 5. 5. Omission of iron from the culture medium, produced porphyrin accumulation and prevented growth. It has been consistently found that, the higher the content of porphyrins, the less the callus growth. Coproporphyrin was the major component of the porphyrins formed in ALA supplemented or iron deficient media. ALA-S and ALA-D were reduced under iron deficiency. 6. 6. The addition of ATP to the media, did not affect porphyrin, protein, and chlorophyll synthesis, growth or ALA-D, but Suc.CoA-S, ALA-S and PBGase activities diminished. 7. 7. Gibberelic acid produced a measurable increase of PBGase, while diminished Suc.CoA-S and ALA-S. 8. 8. Succinate increased growth and inhibited ALA-S and ALA-D. 9. 9. Carbonyl cyanide m-chlorophenyl hydrazine (CCCP), added to the medium produced accumulation of porphyrins, consequently, ALA-S was greatly inhibited and growth prevented. PBGase was also diminished. 10. 10. Coproporphyrinogenase and Decarboxylases activities were hardly detected in most experiments, and are limiting. 11. 11. The complex pattern of results obtained is discussed. © 1975.
format JOUR
author Del C. Batlle, A.M.
Llambias, E.B.C.
de Xlfra, E.W.
Tigier, H.A.
author_facet Del C. Batlle, A.M.
Llambias, E.B.C.
de Xlfra, E.W.
Tigier, H.A.
author_sort Del C. Batlle, A.M.
title Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
title_short Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
title_full Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
title_fullStr Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
title_full_unstemmed Porphyrin biosynthesis in the soybean callus tissue system-XV. The effect of growth conditions
title_sort porphyrin biosynthesis in the soybean callus tissue system-xv. the effect of growth conditions
url http://hdl.handle.net/20.500.12110/paper_0020711X_v6_n8_p591_DelCBatlle
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AT llambiasebc porphyrinbiosynthesisinthesoybeancallustissuesystemxvtheeffectofgrowthconditions
AT dexlfraew porphyrinbiosynthesisinthesoybeancallustissuesystemxvtheeffectofgrowthconditions
AT tigierha porphyrinbiosynthesisinthesoybeancallustissuesystemxvtheeffectofgrowthconditions
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