Thiol groups of soybean callus succinyl CoA synthetase

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1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of...

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Detalles Bibliográficos
Autores principales: De Xifra, E.A.W., Del C. Batlle, A.M.
Formato: JOUR
Materias:
5
5′-dithio-bis-(2-nitrobenzoic acid)
enzyme inactivation
kinetics
molecular weight changes
soybean callus tissue
subunits
Succinyl-CoA synthetase
thiol groups
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974.

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