Thiol groups of soybean callus succinyl CoA synthetase

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1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of...

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Autores principales: De Xifra, E.A.W., Del C. Batlle, A.M.
Formato: JOUR
Materias:
5
5′-dithio-bis-(2-nitrobenzoic acid)
enzyme inactivation
kinetics
molecular weight changes
soybean callus tissue
subunits
Succinyl-CoA synthetase
thiol groups
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_0020711X_v5_n2_p129_DeXifra
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spelling todo:paper_0020711X_v5_n2_p129_DeXifra2023-10-03T14:18:09Z Thiol groups of soybean callus succinyl CoA synthetase De Xifra, E.A.W. Del C. Batlle, A.M. 5 5′-dithio-bis-(2-nitrobenzoic acid) enzyme inactivation kinetics molecular weight changes soybean callus tissue subunits Succinyl-CoA synthetase thiol groups 1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974. Fil:Del C. Batlle, A.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic 5
5′-dithio-bis-(2-nitrobenzoic acid)
enzyme inactivation
kinetics
molecular weight changes
soybean callus tissue
subunits
Succinyl-CoA synthetase
thiol groups
spellingShingle 5
5′-dithio-bis-(2-nitrobenzoic acid)
enzyme inactivation
kinetics
molecular weight changes
soybean callus tissue
subunits
Succinyl-CoA synthetase
thiol groups
De Xifra, E.A.W.
Del C. Batlle, A.M.
Thiol groups of soybean callus succinyl CoA synthetase
topic_facet 5
5′-dithio-bis-(2-nitrobenzoic acid)
enzyme inactivation
kinetics
molecular weight changes
soybean callus tissue
subunits
Succinyl-CoA synthetase
thiol groups
description 1. 1. The reaction between 5,5′-dithio-bis-(2-nitrobenzoic acid) (DTNB) and the SH groups of soybean callus succinyl-CoA synthetase has been investigated. At pH 8-6 and 25° C, four SH groups are titrable with DTNB in the native enzyme. No additional thiol groups have been revealed after unfolding of the protein with 8 M urea. 2. 2. The loss of enzymatic activity paralleled the decrease in the number of free SH groups. As with p-mercuribenzoate and other mercurials, reaction with DTNB also resulted in dissociation of the enzyme into subunits. © 1974.
format JOUR
author De Xifra, E.A.W.
Del C. Batlle, A.M.
author_facet De Xifra, E.A.W.
Del C. Batlle, A.M.
author_sort De Xifra, E.A.W.
title Thiol groups of soybean callus succinyl CoA synthetase
title_short Thiol groups of soybean callus succinyl CoA synthetase
title_full Thiol groups of soybean callus succinyl CoA synthetase
title_fullStr Thiol groups of soybean callus succinyl CoA synthetase
title_full_unstemmed Thiol groups of soybean callus succinyl CoA synthetase
title_sort thiol groups of soybean callus succinyl coa synthetase
url http://hdl.handle.net/20.500.12110/paper_0020711X_v5_n2_p129_DeXifra
work_keys_str_mv AT dexifraeaw thiolgroupsofsoybeancallussuccinylcoasynthetase
AT delcbatlleam thiolgroupsofsoybeancallussuccinylcoasynthetase
_version_ 1807318801969053696

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