δ-Aminolevulinic acid transport in Saccharomyces cerevisiae

1. 1. This work represents the first approach to characterize the transport system of haem pathway precursors, such as δ-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. 2. ALA transport occurs unidirectionally by a sole active system w...

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Autores principales:	Moretti, M.B., García, S.C., Stella, C., Ramos, E., Batlle, A.M.d.C.
Formato:	JOUR
Materias:	Saccharomyces cerevisiae aminolevulinic acid article nonhuman transport kinetics
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0020711X_v25_n12_p1917_Moretti
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_0020711X_v25_n12_p1917_Moretti
record_format	dspace
spelling	todo:paper_0020711X_v25_n12_p1917_Moretti2023-10-03T14:18:08Z δ-Aminolevulinic acid transport in Saccharomyces cerevisiae Moretti, M.B. García, S.C. Stella, C. Ramos, E. Batlle, A.M.d.C. Saccharomyces cerevisiae Saccharomyces cerevisiae aminolevulinic acid article nonhuman Saccharomyces cerevisiae transport kinetics 1. 1. This work represents the first approach to characterize the transport system of haem pathway precursors, such as δ-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. 2. ALA transport occurs unidirectionally by a sole active system with an apparent KM of 0.10 mM, at the optimum pH of 5.0. ALA uptake is influenced by both the carbon and nitrogen source; this suggests a rather complex regulation mechanism. 3. 3. This transport is not mediated by the general amino acid permease (GAP). 4. 4. ALA uptake is strongly inhibited by compounds harboring a methyl-amine terminus suggesting that this group is essential for ALA transport; however, the electric environment of the carboxylic group may be also important for the interaction between ALA and its transporter active site. 5. 5. We have found differences in ALA transport which would indicate a different regulation mechanism for this system in both strain cells. © 1993. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v25_n12_p1917_Moretti
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Saccharomyces cerevisiae Saccharomyces cerevisiae aminolevulinic acid article nonhuman Saccharomyces cerevisiae transport kinetics
spellingShingle	Saccharomyces cerevisiae Saccharomyces cerevisiae aminolevulinic acid article nonhuman Saccharomyces cerevisiae transport kinetics Moretti, M.B. García, S.C. Stella, C. Ramos, E. Batlle, A.M.d.C. δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
topic_facet	Saccharomyces cerevisiae Saccharomyces cerevisiae aminolevulinic acid article nonhuman Saccharomyces cerevisiae transport kinetics
description	1. 1. This work represents the first approach to characterize the transport system of haem pathway precursors, such as δ-aminolevulinic acid (ALA), in two strains of Saccharomyces cerevisiae, a wild type, D27, and a HEM R+ mutant. 2. 2. ALA transport occurs unidirectionally by a sole active system with an apparent KM of 0.10 mM, at the optimum pH of 5.0. ALA uptake is influenced by both the carbon and nitrogen source; this suggests a rather complex regulation mechanism. 3. 3. This transport is not mediated by the general amino acid permease (GAP). 4. 4. ALA uptake is strongly inhibited by compounds harboring a methyl-amine terminus suggesting that this group is essential for ALA transport; however, the electric environment of the carboxylic group may be also important for the interaction between ALA and its transporter active site. 5. 5. We have found differences in ALA transport which would indicate a different regulation mechanism for this system in both strain cells. © 1993.
format	JOUR
author	Moretti, M.B. García, S.C. Stella, C. Ramos, E. Batlle, A.M.d.C.
author_facet	Moretti, M.B. García, S.C. Stella, C. Ramos, E. Batlle, A.M.d.C.
author_sort	Moretti, M.B.
title	δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
title_short	δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
title_full	δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
title_fullStr	δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
title_full_unstemmed	δ-Aminolevulinic acid transport in Saccharomyces cerevisiae
title_sort	δ-aminolevulinic acid transport in saccharomyces cerevisiae
url	http://hdl.handle.net/20.500.12110/paper_0020711X_v25_n12_p1917_Moretti
work_keys_str_mv	AT morettimb daminolevulinicacidtransportinsaccharomycescerevisiae AT garciasc daminolevulinicacidtransportinsaccharomycescerevisiae AT stellac daminolevulinicacidtransportinsaccharomycescerevisiae AT ramose daminolevulinicacidtransportinsaccharomycescerevisiae AT batlleamdc daminolevulinicacidtransportinsaccharomycescerevisiae
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δ-Aminolevulinic acid transport in Saccharomyces cerevisiae

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