Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)

1. 1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.3.1) from erythrocytes was investigated in DMD-patients and DMD-carriers. 2. 2. A different allosteric behaviour in the p-nitrophenylphosphatase from DMD-patients and DMD-carriers compared to controls is supported by the following...

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Autores principales:	Goldemberg, A.L., García, A.M., Fernández, H., Fortunato, M., Sánchez, J.J., Trucco, R.E.
Formato:	JOUR
Materias:	alkaline phosphatase allosterism case report duchenne muscular dystrophy erythrocyte heterozygote human human cell 4-Nitrophenylphosphatase Alkaline Phosphatase Allosteric Regulation Erythrocyte Membrane Female Fluorides Heterozygote Human In Vitro Kinetics Magnesium Male Muscular Dystrophies Phosphoric Monoester Hydrolases Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0020711X_v20_n7_p703_Goldemberg
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_0020711X_v20_n7_p703_Goldemberg2023-10-03T14:17:59Z Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens) Goldemberg, A.L. García, A.M. Fernández, H. Fortunato, M. Sánchez, J.J. Trucco, R.E. alkaline phosphatase allosterism case report duchenne muscular dystrophy erythrocyte heterozygote human human cell 4-Nitrophenylphosphatase Alkaline Phosphatase Allosteric Regulation Erythrocyte Membrane Female Fluorides Heterozygote Human In Vitro Kinetics Magnesium Male Muscular Dystrophies Phosphoric Monoester Hydrolases Support, Non-U.S. Gov't 1. 1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.3.1) from erythrocytes was investigated in DMD-patients and DMD-carriers. 2. 2. A different allosteric behaviour in the p-nitrophenylphosphatase from DMD-patients and DMD-carriers compared to controls is supported by the following finclings: (a) values of n altered in F- inhibition of (K+-activated p-nitrophenylphosphatase with Hill coefficients -1.5, -2.2 and -3.1; (b) heterotropic effect of increased concentration of Mg2+ on F- inhibition which is reverted by K+ in DMD-carriers and in control, but not in DMD-patients. 3. 3. Evidence is presented showing that in DMD-patients and in DMD-carriers the interaction membrane-enzyme is different from the corresponcling controls. © 1988. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v20_n7_p703_Goldemberg
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	alkaline phosphatase allosterism case report duchenne muscular dystrophy erythrocyte heterozygote human human cell 4-Nitrophenylphosphatase Alkaline Phosphatase Allosteric Regulation Erythrocyte Membrane Female Fluorides Heterozygote Human In Vitro Kinetics Magnesium Male Muscular Dystrophies Phosphoric Monoester Hydrolases Support, Non-U.S. Gov't
spellingShingle	alkaline phosphatase allosterism case report duchenne muscular dystrophy erythrocyte heterozygote human human cell 4-Nitrophenylphosphatase Alkaline Phosphatase Allosteric Regulation Erythrocyte Membrane Female Fluorides Heterozygote Human In Vitro Kinetics Magnesium Male Muscular Dystrophies Phosphoric Monoester Hydrolases Support, Non-U.S. Gov't Goldemberg, A.L. García, A.M. Fernández, H. Fortunato, M. Sánchez, J.J. Trucco, R.E. Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
topic_facet	alkaline phosphatase allosterism case report duchenne muscular dystrophy erythrocyte heterozygote human human cell 4-Nitrophenylphosphatase Alkaline Phosphatase Allosteric Regulation Erythrocyte Membrane Female Fluorides Heterozygote Human In Vitro Kinetics Magnesium Male Muscular Dystrophies Phosphoric Monoester Hydrolases Support, Non-U.S. Gov't
description	1. 1. The kinetic properties of the p-nitrophenylphosphatase (EC 3.1.3.1) from erythrocytes was investigated in DMD-patients and DMD-carriers. 2. 2. A different allosteric behaviour in the p-nitrophenylphosphatase from DMD-patients and DMD-carriers compared to controls is supported by the following finclings: (a) values of n altered in F- inhibition of (K+-activated p-nitrophenylphosphatase with Hill coefficients -1.5, -2.2 and -3.1; (b) heterotropic effect of increased concentration of Mg2+ on F- inhibition which is reverted by K+ in DMD-carriers and in control, but not in DMD-patients. 3. 3. Evidence is presented showing that in DMD-patients and in DMD-carriers the interaction membrane-enzyme is different from the corresponcling controls. © 1988.
format	JOUR
author	Goldemberg, A.L. García, A.M. Fernández, H. Fortunato, M. Sánchez, J.J. Trucco, R.E.
author_facet	Goldemberg, A.L. García, A.M. Fernández, H. Fortunato, M. Sánchez, J.J. Trucco, R.E.
author_sort	Goldemberg, A.L.
title	Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
title_short	Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
title_full	Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
title_fullStr	Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
title_full_unstemmed	Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)
title_sort	allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (dmd) patients and duchenne muscular dystrophy carriers (homo sapiens)
url	http://hdl.handle.net/20.500.12110/paper_0020711X_v20_n7_p703_Goldemberg
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Allosteric transition of erythrocyte alkaline phosphatase from duchenne muscular dystrophy (DMD) patients and duchenne muscular dystrophy carriers (Homo sapiens)

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