Human red cell porphobilinogen deaminase. a simpler method of purification and some unusual properties

1. 1. A simpler method for purifying human red cell deaminase, using a mixture of n-butanol and chloroform, which denatures hemoglobin, followed by ammonium sulphate fractionation, heat treatment. Sephadex G-100 and DEAE-cellulose chromatography, yielding a 3400 fold purified enzyme is described. 2....

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Detalles Bibliográficos
Autores principales:	Fumagalli, S.A., Kotler, M.L., Rossetti, M.V., Del C. Batlle, A.M.
Formato:	JOUR
Materias:	porphobilinogen deaminase uroporphyrinogen blood and hemopoietic system erythrocyte human human cell 1-Butanol Ammonia-Lyases Ammonium Sulfate Butanols Chloroform Chromatography Drug Stability Erythrocytes Human Hydrogen-Ion Concentration Hydroxymethylbilane Synthase Kinetics Magnesium Magnesium Chloride Molecular Weight Oxygen Sodium Chloride Support, Non-U.S. Gov't Temperature Uroporphyrinogens
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_0020711X_v17_n4_p485_Fumagalli
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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