The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria
1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a su...
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todo:paper_0020711X_v12_n5-6_p791_Brocklehurst2023-10-03T14:17:43Z The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria Brocklehurst, D. Wider De Xifra, E.A. Batlle, A. blood and hemopoietic system erythrocyte methodology porphyria uroporphyrinogen synthase Acute Disease Adult Aged Ammonia-Lyases Chronic Disease Comparative Study Enzyme Tests Erythrocytes Female Human Hydroxymethylbilane Synthase Kinetics Male Pedigree Porphyria 1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a substrate concentration of delta aminolaevulinic acid (ALA) of 1000nmol/ml is required. Interpretation of the results obtained by this method is discussed and its use in the detection of both latent and acute intermittent porphyria is demonstrated. Comparative studies were carried out by using the Batlle et al. (1978) method and a modification employing ALA as substrate. © 1980. Fil:Wider De Xifra, E.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Batlle, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p791_Brocklehurst |
| institution |
Universidad de Buenos Aires |
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I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
blood and hemopoietic system erythrocyte methodology porphyria uroporphyrinogen synthase Acute Disease Adult Aged Ammonia-Lyases Chronic Disease Comparative Study Enzyme Tests Erythrocytes Female Human Hydroxymethylbilane Synthase Kinetics Male Pedigree Porphyria |
| spellingShingle |
blood and hemopoietic system erythrocyte methodology porphyria uroporphyrinogen synthase Acute Disease Adult Aged Ammonia-Lyases Chronic Disease Comparative Study Enzyme Tests Erythrocytes Female Human Hydroxymethylbilane Synthase Kinetics Male Pedigree Porphyria Brocklehurst, D. Wider De Xifra, E.A. Batlle, A. The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| topic_facet |
blood and hemopoietic system erythrocyte methodology porphyria uroporphyrinogen synthase Acute Disease Adult Aged Ammonia-Lyases Chronic Disease Comparative Study Enzyme Tests Erythrocytes Female Human Hydroxymethylbilane Synthase Kinetics Male Pedigree Porphyria |
| description |
1. 1. This paper confirms the increase in sensitivity obtained for erythrocyte UIS measurement by pre-incubation of the red cells in a 0.2% Triton-X 100 solution containing 1 mmol/1 ZnSO4 and dithiothreitol as described by Piepkorn et al. (1978). 2. 2. To achieve optimal precision in this assay a substrate concentration of delta aminolaevulinic acid (ALA) of 1000nmol/ml is required. Interpretation of the results obtained by this method is discussed and its use in the detection of both latent and acute intermittent porphyria is demonstrated. Comparative studies were carried out by using the Batlle et al. (1978) method and a modification employing ALA as substrate. © 1980. |
| format |
JOUR |
| author |
Brocklehurst, D. Wider De Xifra, E.A. Batlle, A. |
| author_facet |
Brocklehurst, D. Wider De Xifra, E.A. Batlle, A. |
| author_sort |
Brocklehurst, D. |
| title |
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| title_short |
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| title_full |
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| title_fullStr |
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| title_full_unstemmed |
The measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| title_sort |
measurement of erythrocyte uroporphyrinogen i synthase in the diagnosis of latent and acute intermittent porphyria |
| url |
http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p791_Brocklehurst |
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| _version_ |
1782026060711329792 |