Porphyrinogen carboxy-lyase from chick embryo liver-in vivo effect of heptachlor and lindane
1. 1. Heptachlor and lindane are widespread chlorinated insecticides. But porphyrinogen carboxy-lyase seems to be a key enzyme in the porphyria produced by chlorinated compounds in mammals. It was then considered interesting to perform the present investigation, the purpose of which was to study: (a...
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| Autores principales: | , |
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| Formato: | JOUR |
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_0020711X_v12_n5-6_p1033_Taira |
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| Sumario: | 1. 1. Heptachlor and lindane are widespread chlorinated insecticides. But porphyrinogen carboxy-lyase seems to be a key enzyme in the porphyria produced by chlorinated compounds in mammals. It was then considered interesting to perform the present investigation, the purpose of which was to study: (a) the porphyrinogenic ability of heptachlor and lindane when they were administered in vivo to whole chick embryo and (b) the in vivo effect of heptachlor and lindane on porphyrinogen carboxy-lyase activity. 2. 2. The results obtained showed that both compounds produce a significant accumulation of hepatic porphyrins, that produced by lindane being greater than that produced by heptachlor. 3. 3. The effect of heptachlor and lindane administration on porphyrinogen carboxy-lyase was studied in embryos from New Hampshire and Leghorn strains and in New Hampshire 1-day old chicks. The influence of phenobarbital and SKF-525 A pretreatment on the response of porphyrinogen carboxy-lyase to these drugs was also studied in New Hampshire chick embryos. The results indicate that the drugs do not produce decreased enzyme activity in the New Hampshire embryos, while a decrease of enzyme activity in the White Leghorn embryos and in the New Hampshire new-born chicks was observed. Results from pretreatment assays suggest that the decrease of porphyrinogen carboxy-lyase activity seems to be associated with the formation of a drug metabolite. 4. 4. It is concluded that heptachlor and lindane are porphyrinogenic drugs and that the response of decarboxylating enzyme to them seems to be dependent on: (a) the strain, (b) the development of the animal and (c) the drug metabolization system; some of the lindane and heptachlor metabolites being responsible for hepatic porphyrinogen carboxy-lyase decrease. © 1980. |
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