BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis

Brucella is responsible for brucellosis, one of the most common zoonoses worldwide that causes important economic losses in several countries. Increasing evidence indicates that adhesion of Brucella spp. to host cells is an important step to establish infection. We have previously shown that the Bma...

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Autores principales: Ruiz-Ranwez, V., Posadas, D.M., Van der Henst, C., Estein, S.M., Arocena, G.M., Abdian, P.L., Martín, F.A., Sieira, R., De Bolle, X., Zorreguieta, A.
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Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00199567_v81_n3_p996_RuizRanwez
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spelling todo:paper_00199567_v81_n3_p996_RuizRanwez2023-10-03T14:16:37Z BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis Ruiz-Ranwez, V. Posadas, D.M. Van der Henst, C. Estein, S.M. Arocena, G.M. Abdian, P.L. Martín, F.A. Sieira, R. De Bolle, X. Zorreguieta, A. adhesin btae protein fibronectin hyaluronic acid protein unclassified drug article bacterial strain bacterial virulence bacterium adherence Brucella suis cell surface computer model epithelium cell Escherichia coli in vitro study in vivo study internalization phenotype priority journal protein binding protein expression protein function protein localization Yersinia enterocolitica Adhesins, Bacterial Animals Antibodies, Bacterial Bacterial Adhesion Brucella suis Brucellosis Carrier Proteins Cell Polarity Escherichia coli Gene Expression Regulation, Bacterial Mice Mice, Inbred BALB C Multigene Family Virulence Brucella is responsible for brucellosis, one of the most common zoonoses worldwide that causes important economic losses in several countries. Increasing evidence indicates that adhesion of Brucella spp. to host cells is an important step to establish infection. We have previously shown that the BmaC unipolar monomeric autotransporter mediates the binding of Brucella suis to host cells through cell-associated fibronectin. Our genome analysis shows that the B. suis genome encodes several additional potential adhesins. In this work, we characterized a predicted trimeric autotransporter that we named BtaE. By expressing btaE in a nonadherent Escherichia coli strain and by phenotypic characterization of a B. suis δbtaE mutant, we showed that BtaE is involved in the binding of B. suis to hyaluronic acid. The B. suis δbtaE mutant exhibited a reduction in the adhesion to HeLa and A549 epithelial cells compared with the wild-type strain, and it was outcompeted by the wild-type strain in the binding to HeLa cells. The knockout btaE mutant showed an attenuated phenotype in the mouse model, indicating that BtaE is required for full virulence. BtaE was immunodetected on the bacterial surface at one cell pole. Using old and new pole markers, we observed that both the BmaC and BtaE adhesins are consistently associated with the new cell pole, suggesting that, in Brucella, the new pole is functionally differentiated for adhesion. This is consistent with the inherent polarization of this bacterium, and its role in the invasion process. © 2013, American Society for Microbiology. Fil:Posadas, D.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Abdian, P.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Martín, F.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Zorreguieta, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00199567_v81_n3_p996_RuizRanwez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic adhesin
btae protein
fibronectin
hyaluronic acid
protein
unclassified drug
article
bacterial strain
bacterial virulence
bacterium adherence
Brucella suis
cell surface
computer model
epithelium cell
Escherichia coli
in vitro study
in vivo study
internalization
phenotype
priority journal
protein binding
protein expression
protein function
protein localization
Yersinia enterocolitica
Adhesins, Bacterial
Animals
Antibodies, Bacterial
Bacterial Adhesion
Brucella suis
Brucellosis
Carrier Proteins
Cell Polarity
Escherichia coli
Gene Expression Regulation, Bacterial
Mice
Mice, Inbred BALB C
Multigene Family
Virulence
spellingShingle adhesin
btae protein
fibronectin
hyaluronic acid
protein
unclassified drug
article
bacterial strain
bacterial virulence
bacterium adherence
Brucella suis
cell surface
computer model
epithelium cell
Escherichia coli
in vitro study
in vivo study
internalization
phenotype
priority journal
protein binding
protein expression
protein function
protein localization
Yersinia enterocolitica
Adhesins, Bacterial
Animals
Antibodies, Bacterial
Bacterial Adhesion
Brucella suis
Brucellosis
Carrier Proteins
Cell Polarity
Escherichia coli
Gene Expression Regulation, Bacterial
Mice
Mice, Inbred BALB C
Multigene Family
Virulence
Ruiz-Ranwez, V.
Posadas, D.M.
Van der Henst, C.
Estein, S.M.
Arocena, G.M.
Abdian, P.L.
Martín, F.A.
Sieira, R.
De Bolle, X.
Zorreguieta, A.
BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
topic_facet adhesin
btae protein
fibronectin
hyaluronic acid
protein
unclassified drug
article
bacterial strain
bacterial virulence
bacterium adherence
Brucella suis
cell surface
computer model
epithelium cell
Escherichia coli
in vitro study
in vivo study
internalization
phenotype
priority journal
protein binding
protein expression
protein function
protein localization
Yersinia enterocolitica
Adhesins, Bacterial
Animals
Antibodies, Bacterial
Bacterial Adhesion
Brucella suis
Brucellosis
Carrier Proteins
Cell Polarity
Escherichia coli
Gene Expression Regulation, Bacterial
Mice
Mice, Inbred BALB C
Multigene Family
Virulence
description Brucella is responsible for brucellosis, one of the most common zoonoses worldwide that causes important economic losses in several countries. Increasing evidence indicates that adhesion of Brucella spp. to host cells is an important step to establish infection. We have previously shown that the BmaC unipolar monomeric autotransporter mediates the binding of Brucella suis to host cells through cell-associated fibronectin. Our genome analysis shows that the B. suis genome encodes several additional potential adhesins. In this work, we characterized a predicted trimeric autotransporter that we named BtaE. By expressing btaE in a nonadherent Escherichia coli strain and by phenotypic characterization of a B. suis δbtaE mutant, we showed that BtaE is involved in the binding of B. suis to hyaluronic acid. The B. suis δbtaE mutant exhibited a reduction in the adhesion to HeLa and A549 epithelial cells compared with the wild-type strain, and it was outcompeted by the wild-type strain in the binding to HeLa cells. The knockout btaE mutant showed an attenuated phenotype in the mouse model, indicating that BtaE is required for full virulence. BtaE was immunodetected on the bacterial surface at one cell pole. Using old and new pole markers, we observed that both the BmaC and BtaE adhesins are consistently associated with the new cell pole, suggesting that, in Brucella, the new pole is functionally differentiated for adhesion. This is consistent with the inherent polarization of this bacterium, and its role in the invasion process. © 2013, American Society for Microbiology.
format JOUR
author Ruiz-Ranwez, V.
Posadas, D.M.
Van der Henst, C.
Estein, S.M.
Arocena, G.M.
Abdian, P.L.
Martín, F.A.
Sieira, R.
De Bolle, X.
Zorreguieta, A.
author_facet Ruiz-Ranwez, V.
Posadas, D.M.
Van der Henst, C.
Estein, S.M.
Arocena, G.M.
Abdian, P.L.
Martín, F.A.
Sieira, R.
De Bolle, X.
Zorreguieta, A.
author_sort Ruiz-Ranwez, V.
title BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
title_short BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
title_full BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
title_fullStr BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
title_full_unstemmed BtaE, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of Brucella suis
title_sort btae, an adhesin that belongs to the trimeric autotransporter family, is required for full virulence and defines a specific adhesive pole of brucella suis
url http://hdl.handle.net/20.500.12110/paper_00199567_v81_n3_p996_RuizRanwez
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