Nitric oxide and macrophage antiviral extrinsic activity
In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated,...
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todo:paper_00192805_v98_n3_p363_Benencia2023-10-03T14:16:34Z Nitric oxide and macrophage antiviral extrinsic activity Benencia, F. Courreges, M.C. n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-L-arginine acetate (L-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of L-NMA was reversed by the addition of L-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, L-NMA treatment resulted in the aggravation of HSV1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00192805_v98_n3_p363_Benencia |
institution |
Universidad de Buenos Aires |
institution_str |
I-28 |
repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
topic |
n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication |
spellingShingle |
n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication Benencia, F. Courreges, M.C. Nitric oxide and macrophage antiviral extrinsic activity |
topic_facet |
n(g) methylarginine nitric oxide animal cell animal experiment animal model article controlled study female herpes simplex keratitis herpes simplex virus 1 herpes simplex virus 2 host resistance immunoreactivity infection resistance macrophage activation mouse nonhuman priority journal signal transduction Animals Cercopithecus aethiops Dose-Response Relationship, Drug Female Herpesvirus 1, Human Herpesvirus 2, Human Keratitis, Herpetic Macrophage Activation Macrophages, Peritoneal Mice Mice, Inbred BALB C Nitric Oxide Nitrites omega-N-Methylarginine Vero Cells Virus Replication |
description |
In this study we evaluated the relationship between nitric oxide (NO) and macrophage antiviral extrinsic activity. Macrophages activated by intraperitoneal injection of herpes simplex virus-2 (HSV-2), showed both extrinsic antiviral activity and high nitrite production in contrast to non-activated, resident macrophages. The extrinsic antiviral activity was observed in cultures of Vero cells infected with HSV-1 and HSV-2. The NO inhibitor N-monomethyl-L-arginine acetate (L-NMA) impaired the antiviral activity of HSV-elicited macrophages. The effect was dose dependent and correlated with a reduction of nitrite in the culture media. The effect of L-NMA was reversed by the addition of L-arginine. These data indicate that NO could be responsible for the described activity. Furthermore, L-NMA treatment resulted in the aggravation of HSV1-induced keratitis in the mouse model, supporting a defensive role of NO in the pathogenesis of HSV-1 corneal infection. |
format |
JOUR |
author |
Benencia, F. Courreges, M.C. |
author_facet |
Benencia, F. Courreges, M.C. |
author_sort |
Benencia, F. |
title |
Nitric oxide and macrophage antiviral extrinsic activity |
title_short |
Nitric oxide and macrophage antiviral extrinsic activity |
title_full |
Nitric oxide and macrophage antiviral extrinsic activity |
title_fullStr |
Nitric oxide and macrophage antiviral extrinsic activity |
title_full_unstemmed |
Nitric oxide and macrophage antiviral extrinsic activity |
title_sort |
nitric oxide and macrophage antiviral extrinsic activity |
url |
http://hdl.handle.net/20.500.12110/paper_00192805_v98_n3_p363_Benencia |
work_keys_str_mv |
AT benenciaf nitricoxideandmacrophageantiviralextrinsicactivity AT courregesmc nitricoxideandmacrophageantiviralextrinsicactivity |
_version_ |
1782026202432667648 |