Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation

Serine leucocyte proteinase inhibitor (SLPI) is the main serine proteinase inhibitor produced by epithelial cells and has been shown to be a pleiotropic molecule with anti-inflammatory and microbicidal activities. However, the role of SLPI on the adaptive immune response is not well established. The...

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Autores principales:	Guerrieri, D., Tateosian, N.L., Maffía, P.C., Reiteri, R.M., Amiano, N.O., Costa, M.J., Villalonga, X., Sanchez, M.L., Estein, S.M., Garcia, V.E., Sallenave, J.-M., Chuluyan, H.E.
Formato:	JOUR
Materias:	Inflammation Innate immunity Macrophages/monocytes Proteases Serine leucocyte proteinase inhibitor T cells T helper type 1 CD18 antigen gamma interferon interleukin 10 interleukin 12 interleukin 2 interleukin 4 interleukin 6 major histocompatibility antigen class 2 mitogenic agent monoclonal antibody OKT 3 serine leucocyte proteinase inhibtor serine proteinase inhibitor thymidine unclassified drug antigen expression article CD4+ T lymphocyte CD8+ T lymphocyte cell culture cell proliferation cytokine production cytokine release human human cell immune response inflammation peripheral blood mononuclear cell priority journal Th1 cell CD4-Positive T-Lymphocytes Cell Proliferation Cytokines Humans Leukocytes, Mononuclear Serine Proteinase Inhibitors Structure-Activity Relationship
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00192805_v133_n4_p434_Guerrieri
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00192805_v133_n4_p434_Guerrieri2023-10-03T14:16:33Z Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation Guerrieri, D. Tateosian, N.L. Maffía, P.C. Reiteri, R.M. Amiano, N.O. Costa, M.J. Villalonga, X. Sanchez, M.L. Estein, S.M. Garcia, V.E. Sallenave, J.-M. Chuluyan, H.E. Inflammation Innate immunity Macrophages/monocytes Proteases Serine leucocyte proteinase inhibitor T cells T helper type 1 CD18 antigen gamma interferon interleukin 10 interleukin 12 interleukin 2 interleukin 4 interleukin 6 major histocompatibility antigen class 2 mitogenic agent monoclonal antibody OKT 3 serine leucocyte proteinase inhibtor serine proteinase inhibitor thymidine unclassified drug antigen expression article CD4+ T lymphocyte CD8+ T lymphocyte cell culture cell proliferation cytokine production cytokine release human human cell immune response inflammation peripheral blood mononuclear cell priority journal Th1 cell CD4-Positive T-Lymphocytes Cell Proliferation Cytokines Humans Leukocytes, Mononuclear Serine Proteinase Inhibitors Structure-Activity Relationship Serine leucocyte proteinase inhibitor (SLPI) is the main serine proteinase inhibitor produced by epithelial cells and has been shown to be a pleiotropic molecule with anti-inflammatory and microbicidal activities. However, the role of SLPI on the adaptive immune response is not well established. Therefore, we evaluated the effect of SLPI on lymphocyte proliferation and cytokine production. Human peripheral blood mononuclear cells (PBMC) were treated with mitogens plus SLPI and proliferation was assessed by [3H]thymidine uptake. The SLPI decreased the lymphocyte proliferation induced by interleukin-2 (IL-2) or OKT3 monoclonal antibodies in a dose-dependent manner. Inhibition was not observed when depleting monocytes from the PBMC and it was restored by adding monocytes and SLPI. SLPI-treated monocyte slightly decreased MHC II and increased CD18 expression, and secreted greater amounts of IL-4, IL-6 and IL-10 in the cell culture supernatants. SLPI-treated monocyte culture supernatant inhibited the CD4+ lymphocyte proliferation but did not affect the proliferation of CD8+ cells. Moreover, IL-2 increased T-bet expression and the presence of SLPI significantly decreased it. Finally, SLPI-treated monocyte culture supernatant dramatically decreased interferon-γ but increased IL-4, IL-6 and IL-10 in the presence of IL-2-treated T cells. Our results demonstrate that SLPI target monocytes, which in turn inhibit CD4 lymphocyte proliferation and T helper type 1 cytokine secretion. Overall, these results suggest that SLPI is an alarm protein that modulates not only the innate immune response but also the adaptive immune response. © 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd. Fil:Guerrieri, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Reiteri, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Amiano, N.O. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Garcia, V.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00192805_v133_n4_p434_Guerrieri
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Inflammation Innate immunity Macrophages/monocytes Proteases Serine leucocyte proteinase inhibitor T cells T helper type 1 CD18 antigen gamma interferon interleukin 10 interleukin 12 interleukin 2 interleukin 4 interleukin 6 major histocompatibility antigen class 2 mitogenic agent monoclonal antibody OKT 3 serine leucocyte proteinase inhibtor serine proteinase inhibitor thymidine unclassified drug antigen expression article CD4+ T lymphocyte CD8+ T lymphocyte cell culture cell proliferation cytokine production cytokine release human human cell immune response inflammation peripheral blood mononuclear cell priority journal Th1 cell CD4-Positive T-Lymphocytes Cell Proliferation Cytokines Humans Leukocytes, Mononuclear Serine Proteinase Inhibitors Structure-Activity Relationship
spellingShingle	Inflammation Innate immunity Macrophages/monocytes Proteases Serine leucocyte proteinase inhibitor T cells T helper type 1 CD18 antigen gamma interferon interleukin 10 interleukin 12 interleukin 2 interleukin 4 interleukin 6 major histocompatibility antigen class 2 mitogenic agent monoclonal antibody OKT 3 serine leucocyte proteinase inhibtor serine proteinase inhibitor thymidine unclassified drug antigen expression article CD4+ T lymphocyte CD8+ T lymphocyte cell culture cell proliferation cytokine production cytokine release human human cell immune response inflammation peripheral blood mononuclear cell priority journal Th1 cell CD4-Positive T-Lymphocytes Cell Proliferation Cytokines Humans Leukocytes, Mononuclear Serine Proteinase Inhibitors Structure-Activity Relationship Guerrieri, D. Tateosian, N.L. Maffía, P.C. Reiteri, R.M. Amiano, N.O. Costa, M.J. Villalonga, X. Sanchez, M.L. Estein, S.M. Garcia, V.E. Sallenave, J.-M. Chuluyan, H.E. Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
topic_facet	Inflammation Innate immunity Macrophages/monocytes Proteases Serine leucocyte proteinase inhibitor T cells T helper type 1 CD18 antigen gamma interferon interleukin 10 interleukin 12 interleukin 2 interleukin 4 interleukin 6 major histocompatibility antigen class 2 mitogenic agent monoclonal antibody OKT 3 serine leucocyte proteinase inhibtor serine proteinase inhibitor thymidine unclassified drug antigen expression article CD4+ T lymphocyte CD8+ T lymphocyte cell culture cell proliferation cytokine production cytokine release human human cell immune response inflammation peripheral blood mononuclear cell priority journal Th1 cell CD4-Positive T-Lymphocytes Cell Proliferation Cytokines Humans Leukocytes, Mononuclear Serine Proteinase Inhibitors Structure-Activity Relationship
description	Serine leucocyte proteinase inhibitor (SLPI) is the main serine proteinase inhibitor produced by epithelial cells and has been shown to be a pleiotropic molecule with anti-inflammatory and microbicidal activities. However, the role of SLPI on the adaptive immune response is not well established. Therefore, we evaluated the effect of SLPI on lymphocyte proliferation and cytokine production. Human peripheral blood mononuclear cells (PBMC) were treated with mitogens plus SLPI and proliferation was assessed by [3H]thymidine uptake. The SLPI decreased the lymphocyte proliferation induced by interleukin-2 (IL-2) or OKT3 monoclonal antibodies in a dose-dependent manner. Inhibition was not observed when depleting monocytes from the PBMC and it was restored by adding monocytes and SLPI. SLPI-treated monocyte slightly decreased MHC II and increased CD18 expression, and secreted greater amounts of IL-4, IL-6 and IL-10 in the cell culture supernatants. SLPI-treated monocyte culture supernatant inhibited the CD4+ lymphocyte proliferation but did not affect the proliferation of CD8+ cells. Moreover, IL-2 increased T-bet expression and the presence of SLPI significantly decreased it. Finally, SLPI-treated monocyte culture supernatant dramatically decreased interferon-γ but increased IL-4, IL-6 and IL-10 in the presence of IL-2-treated T cells. Our results demonstrate that SLPI target monocytes, which in turn inhibit CD4 lymphocyte proliferation and T helper type 1 cytokine secretion. Overall, these results suggest that SLPI is an alarm protein that modulates not only the innate immune response but also the adaptive immune response. © 2011 The Authors. Immunology © 2011 Blackwell Publishing Ltd.
format	JOUR
author	Guerrieri, D. Tateosian, N.L. Maffía, P.C. Reiteri, R.M. Amiano, N.O. Costa, M.J. Villalonga, X. Sanchez, M.L. Estein, S.M. Garcia, V.E. Sallenave, J.-M. Chuluyan, H.E.
author_facet	Guerrieri, D. Tateosian, N.L. Maffía, P.C. Reiteri, R.M. Amiano, N.O. Costa, M.J. Villalonga, X. Sanchez, M.L. Estein, S.M. Garcia, V.E. Sallenave, J.-M. Chuluyan, H.E.
author_sort	Guerrieri, D.
title	Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
title_short	Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
title_full	Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
title_fullStr	Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
title_full_unstemmed	Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation
title_sort	serine leucocyte proteinase inhibitor-treated monocyte inhibits human cd4+ lymphocyte proliferation
url	http://hdl.handle.net/20.500.12110/paper_00192805_v133_n4_p434_Guerrieri
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Serine leucocyte proteinase inhibitor-treated monocyte inhibits human CD4+ lymphocyte proliferation

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