Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1
To study the origin of interleukin 1 (IL-1) present in human follicular fluid we determined the percentage of macrophages (MO) and cells with HLA-DR antigen (DR+) present in 22 samples of human follicular fluid (FF) from women undergoing in vitro fertilization, and examined the release of IL-1β by c...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00185043_v27_n11_p495_Baranao |
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todo:paper_00185043_v27_n11_p495_Baranao2023-10-03T14:15:37Z Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 Baranao, R.I. Dain, L. De Fried, E.P. Rumi, L.S. Granulosa cells HLA-DR Antigen Interleukin 1 Macrophages chorionic gonadotropin follitropin HLA DR antigen human menopausal gonadotropin interleukin 1 leuprorelin phytohemagglutinin urofollitropin adult article controlled study female gene expression granulosa cell human human cell macrophage priority journal To study the origin of interleukin 1 (IL-1) present in human follicular fluid we determined the percentage of macrophages (MO) and cells with HLA-DR antigen (DR+) present in 22 samples of human follicular fluid (FF) from women undergoing in vitro fertilization, and examined the release of IL-1β by cultures of purified human granulosa cells (GC). The number of red blood cells (RBC) in the crude preparation was taken as a measure of possible contamination with peripheral blood monocytes (assuming a ratio of one monocyte or MO per 104 RBC). For the evaluation of MO and DR+ cells percentages we employed an indirect immunofluorescence technique using specific monoclonal antibodies. Total cells from FF were purified by Ficoll-Hypaque density gradient centrifugation (δ = 1.076 g/l and GC were purified using a gradient δ = 1.065 g/l. This method reduced the contamination with MO to 0-1%. The spontaneous release of IL-1β was measured by ELISA, We found that FF contained 9.81 ± 1.47% of MO but only 7.85% were ovarian MO. In addition the total percentage of DR+ cells was 17.13 ± 2.35% but only 9.81% corresponded to MO. Therefore about 7.32% of DR+ cells could be GC. Then purified GC (104/0.2 ml/well) were cultured during 24 hours at 37°C in serum free medium (DMEM:F12). IL-1β levels were 84±17 pg/ml and these values were increased by 44% when GC were stimulated with FSH (200 ng/ml). These results suggest that GC produced IL-1β and that the synthesis of this cytokine might be regulated by hormones. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00185043_v27_n11_p495_Baranao |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Granulosa cells HLA-DR Antigen Interleukin 1 Macrophages chorionic gonadotropin follitropin HLA DR antigen human menopausal gonadotropin interleukin 1 leuprorelin phytohemagglutinin urofollitropin adult article controlled study female gene expression granulosa cell human human cell macrophage priority journal |
| spellingShingle |
Granulosa cells HLA-DR Antigen Interleukin 1 Macrophages chorionic gonadotropin follitropin HLA DR antigen human menopausal gonadotropin interleukin 1 leuprorelin phytohemagglutinin urofollitropin adult article controlled study female gene expression granulosa cell human human cell macrophage priority journal Baranao, R.I. Dain, L. De Fried, E.P. Rumi, L.S. Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| topic_facet |
Granulosa cells HLA-DR Antigen Interleukin 1 Macrophages chorionic gonadotropin follitropin HLA DR antigen human menopausal gonadotropin interleukin 1 leuprorelin phytohemagglutinin urofollitropin adult article controlled study female gene expression granulosa cell human human cell macrophage priority journal |
| description |
To study the origin of interleukin 1 (IL-1) present in human follicular fluid we determined the percentage of macrophages (MO) and cells with HLA-DR antigen (DR+) present in 22 samples of human follicular fluid (FF) from women undergoing in vitro fertilization, and examined the release of IL-1β by cultures of purified human granulosa cells (GC). The number of red blood cells (RBC) in the crude preparation was taken as a measure of possible contamination with peripheral blood monocytes (assuming a ratio of one monocyte or MO per 104 RBC). For the evaluation of MO and DR+ cells percentages we employed an indirect immunofluorescence technique using specific monoclonal antibodies. Total cells from FF were purified by Ficoll-Hypaque density gradient centrifugation (δ = 1.076 g/l and GC were purified using a gradient δ = 1.065 g/l. This method reduced the contamination with MO to 0-1%. The spontaneous release of IL-1β was measured by ELISA, We found that FF contained 9.81 ± 1.47% of MO but only 7.85% were ovarian MO. In addition the total percentage of DR+ cells was 17.13 ± 2.35% but only 9.81% corresponded to MO. Therefore about 7.32% of DR+ cells could be GC. Then purified GC (104/0.2 ml/well) were cultured during 24 hours at 37°C in serum free medium (DMEM:F12). IL-1β levels were 84±17 pg/ml and these values were increased by 44% when GC were stimulated with FSH (200 ng/ml). These results suggest that GC produced IL-1β and that the synthesis of this cytokine might be regulated by hormones. |
| format |
JOUR |
| author |
Baranao, R.I. Dain, L. De Fried, E.P. Rumi, L.S. |
| author_facet |
Baranao, R.I. Dain, L. De Fried, E.P. Rumi, L.S. |
| author_sort |
Baranao, R.I. |
| title |
Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| title_short |
Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| title_full |
Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| title_fullStr |
Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| title_full_unstemmed |
Human granulosa cells express HLA-DR antigen and are capable of synthesizing interleukin-1 |
| title_sort |
human granulosa cells express hla-dr antigen and are capable of synthesizing interleukin-1 |
| url |
http://hdl.handle.net/20.500.12110/paper_00185043_v27_n11_p495_Baranao |
| work_keys_str_mv |
AT baranaori humangranulosacellsexpresshladrantigenandarecapableofsynthesizinginterleukin1 AT dainl humangranulosacellsexpresshladrantigenandarecapableofsynthesizinginterleukin1 AT defriedep humangranulosacellsexpresshladrantigenandarecapableofsynthesizinginterleukin1 AT rumils humangranulosacellsexpresshladrantigenandarecapableofsynthesizinginterleukin1 |
| _version_ |
1807323224936022016 |