Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats
All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome stud...
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todo:paper_00166707_v143_n4_p393_Adilardi2023-10-03T14:14:20Z Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats Adilardi, R.S. Ojanguren-Affilastro, A.A. Mattoni, C.I. Mola, L.M. Achiasmatic meiosis Female diffuse stage FISH Holokinetic chromosomes Telomere Buthidae Scorpiones heterochromatin ribosome DNA animal chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin karyotyping male meiosis nucleotide repeat scorpion telomere Animals Chromosome Banding DNA, Ribosomal Female Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Repetitive Sequences, Nucleic Acid Scorpions Telomere All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n = 18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)<inf>n</inf> motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism. © 2015, Springer International Publishing Switzerland. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166707_v143_n4_p393_Adilardi |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Achiasmatic meiosis Female diffuse stage FISH Holokinetic chromosomes Telomere Buthidae Scorpiones heterochromatin ribosome DNA animal chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin karyotyping male meiosis nucleotide repeat scorpion telomere Animals Chromosome Banding DNA, Ribosomal Female Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Repetitive Sequences, Nucleic Acid Scorpions Telomere |
| spellingShingle |
Achiasmatic meiosis Female diffuse stage FISH Holokinetic chromosomes Telomere Buthidae Scorpiones heterochromatin ribosome DNA animal chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin karyotyping male meiosis nucleotide repeat scorpion telomere Animals Chromosome Banding DNA, Ribosomal Female Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Repetitive Sequences, Nucleic Acid Scorpions Telomere Adilardi, R.S. Ojanguren-Affilastro, A.A. Mattoni, C.I. Mola, L.M. Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| topic_facet |
Achiasmatic meiosis Female diffuse stage FISH Holokinetic chromosomes Telomere Buthidae Scorpiones heterochromatin ribosome DNA animal chromosome banding pattern female fluorescence in situ hybridization genetics heterochromatin karyotyping male meiosis nucleotide repeat scorpion telomere Animals Chromosome Banding DNA, Ribosomal Female Heterochromatin In Situ Hybridization, Fluorescence Karyotyping Male Meiosis Repetitive Sequences, Nucleic Acid Scorpions Telomere |
| description |
All cytogenetically studied scorpions present male achiasmatic meiosis and lack heteromorphic sex chromosomes. In contrast, information about female meiosis in scorpions is scarce due to the difficulty of finding meiotic cells. The genus Zabius includes three described species and no chromosome studies have been performed on it until now. We analyzed the constitutive heterochromatin distribution, NORs and telomeric sequences in mitosis and meiosis of males and females of different populations of Zabius fuscus. All specimens presented 2n = 18 holokinetic chromosomes that gradually decreased in size. Male meiosis presented nine bivalents and a polymorphism for one reciprocal translocation in one population. Telomeric signals were detected at every terminal region, confirming also the presence of a (TTAGG)<inf>n</inf> motif in Buthidae. Constitutive heterochromatin was found in three chromosome pairs at a terminal region; moreover, NORs were embedded in the heterochromatic region of the largest pair. Chromosome size and landmarks allowed us to propose the chromosomes involved in the rearrangement. In four females, cells at different prophase I stages were analyzed. We describe a diffuse stage and the presence of ring-shaped bivalents. We discuss the possible origin of these bivalents in the framework of chiasmatic or achiasmatic female meiosis. These results contribute to increase the scarce evidence of female meiosis in scorpions and raise new questions about its mechanism. © 2015, Springer International Publishing Switzerland. |
| format |
JOUR |
| author |
Adilardi, R.S. Ojanguren-Affilastro, A.A. Mattoni, C.I. Mola, L.M. |
| author_facet |
Adilardi, R.S. Ojanguren-Affilastro, A.A. Mattoni, C.I. Mola, L.M. |
| author_sort |
Adilardi, R.S. |
| title |
Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| title_short |
Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| title_full |
Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| title_fullStr |
Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| title_full_unstemmed |
Male and female meiosis in the mountain scorpion Zabius fuscus (Scorpiones, Buthidae): heterochromatin, rDNA and TTAGG telomeric repeats |
| title_sort |
male and female meiosis in the mountain scorpion zabius fuscus (scorpiones, buthidae): heterochromatin, rdna and ttagg telomeric repeats |
| url |
http://hdl.handle.net/20.500.12110/paper_00166707_v143_n4_p393_Adilardi |
| work_keys_str_mv |
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1807323576558157824 |