Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum
GnRH controls vertebrate reproduction in several ways. This hormone not only affects the secretion of gonadotropins from the pituitary gland but also has a direct influence on several gonadal functions such as steroidogenesis, spermatogenesis, and spermiation. In the present paper we have studied th...
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todo:paper_00166480_v127_n2_p174_Canosa2023-10-03T14:14:02Z Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum Canosa, L.F. Pozzi, A.G. Somoza, G.M. Ceballos, N.R. Amphibian-testis GnRH-receptor Gonadotropin-releasing hormone Steroidogenesis Steroidogenic-enzyme Toad chorionic gonadotropin cytochrome P450 gonadorelin gonadotropin protein animal tissue article binding site controlled study enzyme activity hormone action hormone binding hormone release in vitro study male nonhuman priority journal protein binding regulatory mechanism steroidogenesis testis testosterone release toad GnRH controls vertebrate reproduction in several ways. This hormone not only affects the secretion of gonadotropins from the pituitary gland but also has a direct influence on several gonadal functions such as steroidogenesis, spermatogenesis, and spermiation. In the present paper we have studied the in vitro effects of GnRH on the testicular steroidogenesis of Bufo arenarum to ascertain the role of this peptide in the control of the steroidogenic pathway previously described in this species. It was found that GnRH is able to reduce basal as well as hCG-stimulated testosterone release, having an inhibitory effect on P450c17 activity. Thus, GnRH could be involved in the mechanism that regulates the metabolic change in the testicular steroidogenesis. Additionally, testicular GnRH binding site has been characterised, showing a Kd of 34 nM and a maximum binding of 4.7 pmol/mg protein. © 2002 Elsevier Science (USA). All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v127_n2_p174_Canosa |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Amphibian-testis GnRH-receptor Gonadotropin-releasing hormone Steroidogenesis Steroidogenic-enzyme Toad chorionic gonadotropin cytochrome P450 gonadorelin gonadotropin protein animal tissue article binding site controlled study enzyme activity hormone action hormone binding hormone release in vitro study male nonhuman priority journal protein binding regulatory mechanism steroidogenesis testis testosterone release toad |
| spellingShingle |
Amphibian-testis GnRH-receptor Gonadotropin-releasing hormone Steroidogenesis Steroidogenic-enzyme Toad chorionic gonadotropin cytochrome P450 gonadorelin gonadotropin protein animal tissue article binding site controlled study enzyme activity hormone action hormone binding hormone release in vitro study male nonhuman priority journal protein binding regulatory mechanism steroidogenesis testis testosterone release toad Canosa, L.F. Pozzi, A.G. Somoza, G.M. Ceballos, N.R. Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| topic_facet |
Amphibian-testis GnRH-receptor Gonadotropin-releasing hormone Steroidogenesis Steroidogenic-enzyme Toad chorionic gonadotropin cytochrome P450 gonadorelin gonadotropin protein animal tissue article binding site controlled study enzyme activity hormone action hormone binding hormone release in vitro study male nonhuman priority journal protein binding regulatory mechanism steroidogenesis testis testosterone release toad |
| description |
GnRH controls vertebrate reproduction in several ways. This hormone not only affects the secretion of gonadotropins from the pituitary gland but also has a direct influence on several gonadal functions such as steroidogenesis, spermatogenesis, and spermiation. In the present paper we have studied the in vitro effects of GnRH on the testicular steroidogenesis of Bufo arenarum to ascertain the role of this peptide in the control of the steroidogenic pathway previously described in this species. It was found that GnRH is able to reduce basal as well as hCG-stimulated testosterone release, having an inhibitory effect on P450c17 activity. Thus, GnRH could be involved in the mechanism that regulates the metabolic change in the testicular steroidogenesis. Additionally, testicular GnRH binding site has been characterised, showing a Kd of 34 nM and a maximum binding of 4.7 pmol/mg protein. © 2002 Elsevier Science (USA). All rights reserved. |
| format |
JOUR |
| author |
Canosa, L.F. Pozzi, A.G. Somoza, G.M. Ceballos, N.R. |
| author_facet |
Canosa, L.F. Pozzi, A.G. Somoza, G.M. Ceballos, N.R. |
| author_sort |
Canosa, L.F. |
| title |
Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| title_short |
Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| title_full |
Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| title_fullStr |
Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| title_full_unstemmed |
Effects of mGnRH on testicular steroidogenesis in the toad Bufo arenarum |
| title_sort |
effects of mgnrh on testicular steroidogenesis in the toad bufo arenarum |
| url |
http://hdl.handle.net/20.500.12110/paper_00166480_v127_n2_p174_Canosa |
| work_keys_str_mv |
AT canosalf effectsofmgnrhontesticularsteroidogenesisinthetoadbufoarenarum AT pozziag effectsofmgnrhontesticularsteroidogenesisinthetoadbufoarenarum AT somozagm effectsofmgnrhontesticularsteroidogenesisinthetoadbufoarenarum AT ceballosnr effectsofmgnrhontesticularsteroidogenesisinthetoadbufoarenarum |
| _version_ |
1807314522471399424 |