Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis

The effect of methyl farnesoate (MF) on the ovaries of female red swamp crayfish, Procambarus clarkii, undergoing vitellogenesis was determined both in vivo and in vitro. The in vivo assay showed a positive effect of MF on oocyte growth when injected alone and in combination with 17β-estradiol, but...

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Autores principales: Rodríguez, E.M., López Greco, L.S., Medesani, D.A., Laufer, H., Fingerman, M.
Formato: JOUR
Materias:
Crayfish
Hormones
Methyl farnesoate
Vitellogenesis
estradiol
farnesoic acid methyl ester
hormone
hydroxyprogesterone
leucine
methyl group
sesquiterpenoid
steroid
unclassified drug
animal cell
article
competitive inhibition
controlled study
crayfish
female
hormone synthesis
incubation time
negative feedback
nonhuman
oocyte development
ovary development
priority journal
protein synthesis
vitellogenesis
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00166480_v125_n1_p34_Rodriguez
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00166480_v125_n1_p34_Rodriguez
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spelling todo:paper_00166480_v125_n1_p34_Rodriguez2023-10-03T14:14:01Z Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis Rodríguez, E.M. López Greco, L.S. Medesani, D.A. Laufer, H. Fingerman, M. Crayfish Hormones Methyl farnesoate Vitellogenesis estradiol farnesoic acid methyl ester hormone hydroxyprogesterone leucine methyl group sesquiterpenoid steroid unclassified drug animal cell article competitive inhibition controlled study crayfish female hormone synthesis incubation time negative feedback nonhuman oocyte development ovary development priority journal protein synthesis vitellogenesis The effect of methyl farnesoate (MF) on the ovaries of female red swamp crayfish, Procambarus clarkii, undergoing vitellogenesis was determined both in vivo and in vitro. The in vivo assay showed a positive effect of MF on oocyte growth when injected alone and in combination with 17β-estradiol, but not in combination with JHIII or 17α-hydroxyprogesterone. A higher level of incorporation of labeled leucine was induced by MF on isolated pieces of ovary. The same effect was seen when ovary and mandibular organ (MO) were coincubated. These results suggest that MF stimulated the synthesis of vitellin in the ovary of crayfish. In vitro, 17α-hydroxyprogesterone completely suppressed the stimulatory action of the MO on the ovary, suggesting a competitive inhibition between 17α-hydroxyprogesterone and MF on the ovary and/or a negative feedback by that steroid on the MO. © 2002 Elsevier Science (USA). Fil:Rodríguez, E.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:López Greco, L.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medesani, D.A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00166480_v125_n1_p34_Rodriguez
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic Crayfish
Hormones
Methyl farnesoate
Vitellogenesis
estradiol
farnesoic acid methyl ester
hormone
hydroxyprogesterone
leucine
methyl group
sesquiterpenoid
steroid
unclassified drug
animal cell
article
competitive inhibition
controlled study
crayfish
female
hormone synthesis
incubation time
negative feedback
nonhuman
oocyte development
ovary development
priority journal
protein synthesis
vitellogenesis
spellingShingle Crayfish
Hormones
Methyl farnesoate
Vitellogenesis
estradiol
farnesoic acid methyl ester
hormone
hydroxyprogesterone
leucine
methyl group
sesquiterpenoid
steroid
unclassified drug
animal cell
article
competitive inhibition
controlled study
crayfish
female
hormone synthesis
incubation time
negative feedback
nonhuman
oocyte development
ovary development
priority journal
protein synthesis
vitellogenesis
Rodríguez, E.M.
López Greco, L.S.
Medesani, D.A.
Laufer, H.
Fingerman, M.
Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
topic_facet Crayfish
Hormones
Methyl farnesoate
Vitellogenesis
estradiol
farnesoic acid methyl ester
hormone
hydroxyprogesterone
leucine
methyl group
sesquiterpenoid
steroid
unclassified drug
animal cell
article
competitive inhibition
controlled study
crayfish
female
hormone synthesis
incubation time
negative feedback
nonhuman
oocyte development
ovary development
priority journal
protein synthesis
vitellogenesis
description The effect of methyl farnesoate (MF) on the ovaries of female red swamp crayfish, Procambarus clarkii, undergoing vitellogenesis was determined both in vivo and in vitro. The in vivo assay showed a positive effect of MF on oocyte growth when injected alone and in combination with 17β-estradiol, but not in combination with JHIII or 17α-hydroxyprogesterone. A higher level of incorporation of labeled leucine was induced by MF on isolated pieces of ovary. The same effect was seen when ovary and mandibular organ (MO) were coincubated. These results suggest that MF stimulated the synthesis of vitellin in the ovary of crayfish. In vitro, 17α-hydroxyprogesterone completely suppressed the stimulatory action of the MO on the ovary, suggesting a competitive inhibition between 17α-hydroxyprogesterone and MF on the ovary and/or a negative feedback by that steroid on the MO. © 2002 Elsevier Science (USA).
format JOUR
author Rodríguez, E.M.
López Greco, L.S.
Medesani, D.A.
Laufer, H.
Fingerman, M.
author_facet Rodríguez, E.M.
López Greco, L.S.
Medesani, D.A.
Laufer, H.
Fingerman, M.
author_sort Rodríguez, E.M.
title Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
title_short Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
title_full Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
title_fullStr Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
title_full_unstemmed Effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, Procambarus clarkii, during vitellogenesis
title_sort effect of methyl farnesoate, alone and in combination with other hormones, on ovarian growth of the red swamp crayfish, procambarus clarkii, during vitellogenesis
url http://hdl.handle.net/20.500.12110/paper_00166480_v125_n1_p34_Rodriguez
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