Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives
Objective: To evaluate the immunocontraceptive properties of recombinant DE, a sperm epididymal protein involved in fertilization, via an experimental study in rats as a critical step toward the development of a human immunocontraceptive. Design: In vivo study in rats. Setting: Animal care facility...
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todo:paper_00150282_v89_n1_p199_Ellerman2023-10-03T14:13:29Z Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives Ellerman, D.A. Busso, D. Maldera, J.A. Cuasnicú, P.S. CRISP epididymis fertility Immunocontraception sperm recombinant epididymal protein recombinant protein unclassified drug animal tissue article female fertility fertilization immune response immunization immunocontraception in vivo study male nonhuman priority journal rat sperm tissue specificity Animals Antibodies Antibody Formation Antibody Specificity Contraception, Immunologic Contraceptive Agents Epididymal Secretory Proteins Female Fertility Immunization Injections, Intramuscular Injections, Subcutaneous Kinetics Male Membrane Glycoproteins Rats Rats, Wistar Recombinant Proteins Objective: To evaluate the immunocontraceptive properties of recombinant DE, a sperm epididymal protein involved in fertilization, via an experimental study in rats as a critical step toward the development of a human immunocontraceptive. Design: In vivo study in rats. Setting: Animal care facility of an academic research center. Animal(s): Seventy-four 90-day-old Wistar male and female rats distributed into three groups. Intervention(s): Animals received five injections (intramuscular and subcutaneous) of recombinant DE (recDE), native DE (nDE), or MBP (maltose-binding protein). At various times, animals were anesthetized and bled. Main Outcome Measure(s): Anti-DE levels and tissue specificity of sera were evaluated by enzyme-linked immunosorbent assay (ELISA) and Western blot, respectively. Fertility was analyzed by natural mating. The testes and epididymides were analyzed by histology. Result(s): Recombinant DE raised an immune response with the same kinetics and higher anti-DE levels than that elicited by nDE. Sera against recDE recognized epitopes of DE that were different from those recognized by anti-nDE sera but specifically reacted with DE in epididymis and sperm without cross-reacting with other tissues tested. Male and female recDE-injected animals presented a statistically significant reduction in their fertility with no evidence of pathologic effects. Conclusion(s): Recombinant DE is able to both elicit a specific immune response and inhibit male and female fertility, supporting the use of this sperm epididymal protein for the development of an immunocontraceptive approach. © 2008 American Society for Reproductive Medicine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v89_n1_p199_Ellerman |
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Universidad de Buenos Aires |
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I-28 |
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R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
CRISP epididymis fertility Immunocontraception sperm recombinant epididymal protein recombinant protein unclassified drug animal tissue article female fertility fertilization immune response immunization immunocontraception in vivo study male nonhuman priority journal rat sperm tissue specificity Animals Antibodies Antibody Formation Antibody Specificity Contraception, Immunologic Contraceptive Agents Epididymal Secretory Proteins Female Fertility Immunization Injections, Intramuscular Injections, Subcutaneous Kinetics Male Membrane Glycoproteins Rats Rats, Wistar Recombinant Proteins |
| spellingShingle |
CRISP epididymis fertility Immunocontraception sperm recombinant epididymal protein recombinant protein unclassified drug animal tissue article female fertility fertilization immune response immunization immunocontraception in vivo study male nonhuman priority journal rat sperm tissue specificity Animals Antibodies Antibody Formation Antibody Specificity Contraception, Immunologic Contraceptive Agents Epididymal Secretory Proteins Female Fertility Immunization Injections, Intramuscular Injections, Subcutaneous Kinetics Male Membrane Glycoproteins Rats Rats, Wistar Recombinant Proteins Ellerman, D.A. Busso, D. Maldera, J.A. Cuasnicú, P.S. Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| topic_facet |
CRISP epididymis fertility Immunocontraception sperm recombinant epididymal protein recombinant protein unclassified drug animal tissue article female fertility fertilization immune response immunization immunocontraception in vivo study male nonhuman priority journal rat sperm tissue specificity Animals Antibodies Antibody Formation Antibody Specificity Contraception, Immunologic Contraceptive Agents Epididymal Secretory Proteins Female Fertility Immunization Injections, Intramuscular Injections, Subcutaneous Kinetics Male Membrane Glycoproteins Rats Rats, Wistar Recombinant Proteins |
| description |
Objective: To evaluate the immunocontraceptive properties of recombinant DE, a sperm epididymal protein involved in fertilization, via an experimental study in rats as a critical step toward the development of a human immunocontraceptive. Design: In vivo study in rats. Setting: Animal care facility of an academic research center. Animal(s): Seventy-four 90-day-old Wistar male and female rats distributed into three groups. Intervention(s): Animals received five injections (intramuscular and subcutaneous) of recombinant DE (recDE), native DE (nDE), or MBP (maltose-binding protein). At various times, animals were anesthetized and bled. Main Outcome Measure(s): Anti-DE levels and tissue specificity of sera were evaluated by enzyme-linked immunosorbent assay (ELISA) and Western blot, respectively. Fertility was analyzed by natural mating. The testes and epididymides were analyzed by histology. Result(s): Recombinant DE raised an immune response with the same kinetics and higher anti-DE levels than that elicited by nDE. Sera against recDE recognized epitopes of DE that were different from those recognized by anti-nDE sera but specifically reacted with DE in epididymis and sperm without cross-reacting with other tissues tested. Male and female recDE-injected animals presented a statistically significant reduction in their fertility with no evidence of pathologic effects. Conclusion(s): Recombinant DE is able to both elicit a specific immune response and inhibit male and female fertility, supporting the use of this sperm epididymal protein for the development of an immunocontraceptive approach. © 2008 American Society for Reproductive Medicine. |
| format |
JOUR |
| author |
Ellerman, D.A. Busso, D. Maldera, J.A. Cuasnicú, P.S. |
| author_facet |
Ellerman, D.A. Busso, D. Maldera, J.A. Cuasnicú, P.S. |
| author_sort |
Ellerman, D.A. |
| title |
Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| title_short |
Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| title_full |
Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| title_fullStr |
Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| title_full_unstemmed |
Immunocontraceptive properties of recombinant sperm protein DE: implications for the development of novel contraceptives |
| title_sort |
immunocontraceptive properties of recombinant sperm protein de: implications for the development of novel contraceptives |
| url |
http://hdl.handle.net/20.500.12110/paper_00150282_v89_n1_p199_Ellerman |
| work_keys_str_mv |
AT ellermanda immunocontraceptivepropertiesofrecombinantspermproteindeimplicationsforthedevelopmentofnovelcontraceptives AT bussod immunocontraceptivepropertiesofrecombinantspermproteindeimplicationsforthedevelopmentofnovelcontraceptives AT malderaja immunocontraceptivepropertiesofrecombinantspermproteindeimplicationsforthedevelopmentofnovelcontraceptives AT cuasnicups immunocontraceptivepropertiesofrecombinantspermproteindeimplicationsforthedevelopmentofnovelcontraceptives |
| _version_ |
1807320177327472640 |