Calcium requirements for human sperm function in vitro
Objective: To determine extracellular calcium (Ca2+) requirements for the maintenance of human sperm function in vitro. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were col...
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todo:paper_00150282_v79_n6_p1396_MarinBriggiler2023-10-03T14:13:25Z Calcium requirements for human sperm function in vitro Marín-Briggiler, C.I. Gonzalez-Echeverría, F. Buffone, M. Calamera, J.C. Tezón, J.G. Vazquez-Levin, M.H. Acrosome reaction Calcium Capacitation Human spermatozoa Zona pellucida acrosome reaction article controlled study extracellular calcium female fertilization in vitro human human cell incubation time laboratory male normal human oocyte ovary follicle fluid priority journal prospective study protein phosphorylation sperm spermatozoon spermatozoon capacitation spermatozoon motility volunteer zona pellucida Objective: To determine extracellular calcium (Ca2+) requirements for the maintenance of human sperm function in vitro. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET. Intervention(s): Spermatozoa were incubated for ≤18 hours in media containing different CaCl2 concentrations (maximum, 2.5 mM [control]). Main outcome measure(s): Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP). Result(s): Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca2+ concentrations (≥0.58 mM) were required to produce maximum human FF-induced AR in previously capacitated cells and to obtain an adequate sperm-ZP binding. Conclusion(s): Different steps of the fertilization process have distinctive Ca2+ requirements. Whereas 0.22 mM of Ca2+ is sufficient for the development of some capacitation-related events, human FF-induced AR and sperm-ZP interaction require 0.58 mM of this cation. © 2003 by American Society for Reproductive Medicine. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v79_n6_p1396_MarinBriggiler |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Acrosome reaction Calcium Capacitation Human spermatozoa Zona pellucida acrosome reaction article controlled study extracellular calcium female fertilization in vitro human human cell incubation time laboratory male normal human oocyte ovary follicle fluid priority journal prospective study protein phosphorylation sperm spermatozoon spermatozoon capacitation spermatozoon motility volunteer zona pellucida |
| spellingShingle |
Acrosome reaction Calcium Capacitation Human spermatozoa Zona pellucida acrosome reaction article controlled study extracellular calcium female fertilization in vitro human human cell incubation time laboratory male normal human oocyte ovary follicle fluid priority journal prospective study protein phosphorylation sperm spermatozoon spermatozoon capacitation spermatozoon motility volunteer zona pellucida Marín-Briggiler, C.I. Gonzalez-Echeverría, F. Buffone, M. Calamera, J.C. Tezón, J.G. Vazquez-Levin, M.H. Calcium requirements for human sperm function in vitro |
| topic_facet |
Acrosome reaction Calcium Capacitation Human spermatozoa Zona pellucida acrosome reaction article controlled study extracellular calcium female fertilization in vitro human human cell incubation time laboratory male normal human oocyte ovary follicle fluid priority journal prospective study protein phosphorylation sperm spermatozoon spermatozoon capacitation spermatozoon motility volunteer zona pellucida |
| description |
Objective: To determine extracellular calcium (Ca2+) requirements for the maintenance of human sperm function in vitro. Design: Prospective study. Setting: Basic research laboratory. Patient(s): Normozoospermic volunteers provided fresh semen samples; follicular fluid (human FF) and oocytes were collected from women undergoing IVF-ET. Intervention(s): Spermatozoa were incubated for ≤18 hours in media containing different CaCl2 concentrations (maximum, 2.5 mM [control]). Main outcome measure(s): Protein tyrosine phosphorylation patterns, development of hyperactivated motility, induction of the acrosome reaction (AR) in response to human FF, and sperm interaction with homologous zona pellucida (ZP). Result(s): Cells maintained for 18 hours in medium containing ≥0.1 mM of Ca2+ were able to undergo the AR when exposed to human FF in the presence of 2.5 mM of Ca2+. Calcium concentrations of ≥0.22 mM were sufficient to reach protein tyrosine phosphorylation levels and hyperactivated motility values similar to those of controls. Higher Ca2+ concentrations (≥0.58 mM) were required to produce maximum human FF-induced AR in previously capacitated cells and to obtain an adequate sperm-ZP binding. Conclusion(s): Different steps of the fertilization process have distinctive Ca2+ requirements. Whereas 0.22 mM of Ca2+ is sufficient for the development of some capacitation-related events, human FF-induced AR and sperm-ZP interaction require 0.58 mM of this cation. © 2003 by American Society for Reproductive Medicine. |
| format |
JOUR |
| author |
Marín-Briggiler, C.I. Gonzalez-Echeverría, F. Buffone, M. Calamera, J.C. Tezón, J.G. Vazquez-Levin, M.H. |
| author_facet |
Marín-Briggiler, C.I. Gonzalez-Echeverría, F. Buffone, M. Calamera, J.C. Tezón, J.G. Vazquez-Levin, M.H. |
| author_sort |
Marín-Briggiler, C.I. |
| title |
Calcium requirements for human sperm function in vitro |
| title_short |
Calcium requirements for human sperm function in vitro |
| title_full |
Calcium requirements for human sperm function in vitro |
| title_fullStr |
Calcium requirements for human sperm function in vitro |
| title_full_unstemmed |
Calcium requirements for human sperm function in vitro |
| title_sort |
calcium requirements for human sperm function in vitro |
| url |
http://hdl.handle.net/20.500.12110/paper_00150282_v79_n6_p1396_MarinBriggiler |
| work_keys_str_mv |
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1807317328731308032 |