Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation
Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology resea...
| Autores principales: | , , , , , , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro |
| Aporte de: |
| id |
todo:paper_00150282_v78_n6_p1278_Novaro |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00150282_v78_n6_p1278_Novaro2023-10-03T14:13:24Z Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation Novaro, V. Pustovrh, C. Colman-Lerner, A. Radisky, D. Lo Nostro, F. Paz, D. Jawerbaum, A. González, E. Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology research laboratory. Animal(s): Female syngeneic Wistar rats aged 14 weeks. Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors. Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections. Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections. Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation. © 2002 by American Society for Reproductive Medicine. Fil:Novaro, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Pustovrh, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Colman-Lerner, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Lo Nostro, F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paz, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Jawerbaum, A. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus |
| spellingShingle |
Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus Novaro, V. Pustovrh, C. Colman-Lerner, A. Radisky, D. Lo Nostro, F. Paz, D. Jawerbaum, A. González, E. Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| topic_facet |
Decidualization Maternal-embryo interaction Pregnancy Rat uterus gelatinase A nitric oxide nitric oxide donor nitric oxide synthase inhibitor tissue extract animal experiment animal tissue article assay blastocyst controlled study embryo endometrium enzyme activity female immunofluorescence test immunolocalization nidation nonhuman organ culture pregnancy priority journal protein expression rat rat strain uterine tube ligation uterus vagina smear Western blotting zymography Animals Blastocyst Embryo Implantation Enzyme Induction Enzyme Inhibitors Female Matrix Metalloproteinase 2 Nitric Oxide Nitric Oxide Donors Nitric Oxide Synthase omega-N-Methylarginine Organ Culture Techniques Pregnancy Rats Rats, Wistar Uterus |
| description |
Objective: To evaluate a reciprocal signaling interaction initiated by embryo-derived nitric oxide (NO) to facilitate implantation by increased production of gelatinase A (matrix metalloproteinase 2, MMP2) in uterine stroma. Design: Experimental animal studies. Setting: Reproductive-physiology research laboratory. Animal(s): Female syngeneic Wistar rats aged 14 weeks. Intervention(s): Vaginal smears to confirm pregnancy. Oviductal ligature to avoid the descent of blastocysts to the uterine lumen. Plasma exudation assays to locate uterine blastocyst implantation sites. Organ cultures treated with NO donors and nitric oxide synthase (NOS) inhibitors. Main Outcome Measure(s): Expression of MMP2 and NO was assessed by Western blot and zymography of tissue extracts and by immunofluorescence of tissue sections. Result(s): An increase in MMP2 activity was found in uterine extracts in early pregnant rats and was concentrated at implantation sites. Immunolocalization experiments showed that inducible NOS was expressed on the surface of the implanting blastocyst adjacent to the uterine epithelium at the sites of increased MMP2 expression. In organ culture experiments, NO donors were found to increase, whereas NOS inhibitors were found to decrease MMP2 activity in uterine tissue sections. Conclusion(s): Blastocyst-derived NO contributes to the production of uterine-derived MMP2, an essential component of implantation and initiation of placentation. © 2002 by American Society for Reproductive Medicine. |
| format |
JOUR |
| author |
Novaro, V. Pustovrh, C. Colman-Lerner, A. Radisky, D. Lo Nostro, F. Paz, D. Jawerbaum, A. González, E. |
| author_facet |
Novaro, V. Pustovrh, C. Colman-Lerner, A. Radisky, D. Lo Nostro, F. Paz, D. Jawerbaum, A. González, E. |
| author_sort |
Novaro, V. |
| title |
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| title_short |
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| title_full |
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| title_fullStr |
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| title_full_unstemmed |
Nitric oxide induces gelatinase A (matrix metalloproteinase 2) during rat embryo implantation |
| title_sort |
nitric oxide induces gelatinase a (matrix metalloproteinase 2) during rat embryo implantation |
| url |
http://hdl.handle.net/20.500.12110/paper_00150282_v78_n6_p1278_Novaro |
| work_keys_str_mv |
AT novarov nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT pustovrhc nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT colmanlernera nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT radiskyd nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT lonostrof nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT pazd nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT jawerbauma nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation AT gonzaleze nitricoxideinducesgelatinaseamatrixmetalloproteinase2duringratembryoimplantation |
| _version_ |
1807319359309217792 |