Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells

Objective: To examine the effect of a gonadotropin-releasing hormone agonist (GnRH-a), leuprolide acetate (LA), on human chorionic gonadotropin/luteinizing hormone (LH) receptors content and progesterone (P) and estradiol (E2) production in cultured granulosa or luteal cells. Design: Prospective. Se...

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Autores principales: Guerrero, H.E., Stein, P., Asch, R.H., De Fried, E.P., Tesone, M.
Formato: JOUR
Materias:
GnRH-a
granulosa cells
LH receptors
luteal cells
steroidogenesis
aromatase
chorionic gonadotropin
estradiol
gonadorelin
gonadorelin agonist
human menopausal gonadotropin
leuprorelin
luteinizing hormone
luteinizing hormone receptor
progesterone
urofollitropin
animal cell
article
binding site
cell culture
clinical article
controlled study
corpus luteum
echography
enzyme activity
female
granulosa cell
human
human cell
luteal cell
nonhuman
priority journal
radioimmunoassay
rat
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00150282_v59_n4_p803_Guerrero
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00150282_v59_n4_p803_Guerrero
record_format dspace
spelling todo:paper_00150282_v59_n4_p803_Guerrero2023-10-03T14:13:22Z Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells Guerrero, H.E. Stein, P. Asch, R.H. De Fried, E.P. Tesone, M. GnRH-a granulosa cells LH receptors luteal cells steroidogenesis aromatase chorionic gonadotropin estradiol gonadorelin gonadorelin agonist human menopausal gonadotropin leuprorelin luteinizing hormone luteinizing hormone receptor progesterone urofollitropin animal cell article binding site cell culture clinical article controlled study corpus luteum echography enzyme activity female granulosa cell human human cell luteal cell nonhuman priority journal radioimmunoassay rat steroidogenesis Objective: To examine the effect of a gonadotropin-releasing hormone agonist (GnRH-a), leuprolide acetate (LA), on human chorionic gonadotropin/luteinizing hormone (LH) receptors content and progesterone (P) and estradiol (E2) production in cultured granulosa or luteal cells. Design: Prospective. Setting: Private Fertility Clinic and National Research Institute. Patients: Twenty patients undergoing in vitro fertilization or gamete intrafallopian transfer programs. Results: Human chorionic gonadotropin/LH receptors in human granulosa cells increased after 48 hours of culture, and LA inhibited such effect. Leuprolide acetate, 1 ng/mL, in the cultures produced an increase in P production. On the contrary, LA inhibited E2 production. Additionally, the in vivo effect of LA (2 μg/rat per 7 days) was studied in corpus luteum of superovulated rats. Luteal cells from LA- treated rats in culture produced lower P than the controls but showed an increase in aromatase activity. Luteal LH receptors declined after 48 hours of culture with LA. Conclusion: The high doses of gonadotropin necessary to induce ovarian hyperstimulation when GnRH-a is administered could be related with an inhibitory effect of these agonists on LH receptors and aromatase activity. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00150282_v59_n4_p803_Guerrero
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic GnRH-a
granulosa cells
LH receptors
luteal cells
steroidogenesis
aromatase
chorionic gonadotropin
estradiol
gonadorelin
gonadorelin agonist
human menopausal gonadotropin
leuprorelin
luteinizing hormone
luteinizing hormone receptor
progesterone
urofollitropin
animal cell
article
binding site
cell culture
clinical article
controlled study
corpus luteum
echography
enzyme activity
female
granulosa cell
human
human cell
luteal cell
nonhuman
priority journal
radioimmunoassay
rat
steroidogenesis
spellingShingle GnRH-a
granulosa cells
LH receptors
luteal cells
steroidogenesis
aromatase
chorionic gonadotropin
estradiol
gonadorelin
gonadorelin agonist
human menopausal gonadotropin
leuprorelin
luteinizing hormone
luteinizing hormone receptor
progesterone
urofollitropin
animal cell
article
binding site
cell culture
clinical article
controlled study
corpus luteum
echography
enzyme activity
female
granulosa cell
human
human cell
luteal cell
nonhuman
priority journal
radioimmunoassay
rat
steroidogenesis
Guerrero, H.E.
Stein, P.
Asch, R.H.
De Fried, E.P.
Tesone, M.
Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
topic_facet GnRH-a
granulosa cells
LH receptors
luteal cells
steroidogenesis
aromatase
chorionic gonadotropin
estradiol
gonadorelin
gonadorelin agonist
human menopausal gonadotropin
leuprorelin
luteinizing hormone
luteinizing hormone receptor
progesterone
urofollitropin
animal cell
article
binding site
cell culture
clinical article
controlled study
corpus luteum
echography
enzyme activity
female
granulosa cell
human
human cell
luteal cell
nonhuman
priority journal
radioimmunoassay
rat
steroidogenesis
description Objective: To examine the effect of a gonadotropin-releasing hormone agonist (GnRH-a), leuprolide acetate (LA), on human chorionic gonadotropin/luteinizing hormone (LH) receptors content and progesterone (P) and estradiol (E2) production in cultured granulosa or luteal cells. Design: Prospective. Setting: Private Fertility Clinic and National Research Institute. Patients: Twenty patients undergoing in vitro fertilization or gamete intrafallopian transfer programs. Results: Human chorionic gonadotropin/LH receptors in human granulosa cells increased after 48 hours of culture, and LA inhibited such effect. Leuprolide acetate, 1 ng/mL, in the cultures produced an increase in P production. On the contrary, LA inhibited E2 production. Additionally, the in vivo effect of LA (2 μg/rat per 7 days) was studied in corpus luteum of superovulated rats. Luteal cells from LA- treated rats in culture produced lower P than the controls but showed an increase in aromatase activity. Luteal LH receptors declined after 48 hours of culture with LA. Conclusion: The high doses of gonadotropin necessary to induce ovarian hyperstimulation when GnRH-a is administered could be related with an inhibitory effect of these agonists on LH receptors and aromatase activity.
format JOUR
author Guerrero, H.E.
Stein, P.
Asch, R.H.
De Fried, E.P.
Tesone, M.
author_facet Guerrero, H.E.
Stein, P.
Asch, R.H.
De Fried, E.P.
Tesone, M.
author_sort Guerrero, H.E.
title Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
title_short Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
title_full Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
title_fullStr Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
title_full_unstemmed Effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
title_sort effect of a gonadotropin-releasing hormone agonist on luteinizing hormone receptors and steroidogenesis in ovarian cells
url http://hdl.handle.net/20.500.12110/paper_00150282_v59_n4_p803_Guerrero
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