SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation

Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activi...

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Detalles Bibliográficos
Autores principales: Orqueda, A.J., Gatti, C.R., Ogara, M.F., Falzone, T.L.
Formato: JOUR
Materias:
human adipose-derived stem cells
LINE-1
neuroblastoma
neuronal differentiation
SOX-11
DNA
LINE1 DNA
transcription factor Sox11
unclassified drug
5' untranslated region
adipose derived stem cell
adipose mesenchymal stem cell
Article
cell fate
controlled study
DNA sequence
DNA transposition
gene knockdown
genetic transcription
human
human cell
mesenchymal stem cell
nerve cell differentiation
priority journal
protein DNA interaction
retroposon
SH-SY5Y cell line
upregulation
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00145793_v592_n22_p3708_Orqueda
record_format dspace
spelling todo:paper_00145793_v592_n22_p3708_Orqueda2023-10-03T14:13:13Z SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation Orqueda, A.J. Gatti, C.R. Ogara, M.F. Falzone, T.L. human adipose-derived stem cells LINE-1 neuroblastoma neuronal differentiation SOX-11 DNA LINE1 DNA transcription factor Sox11 unclassified drug 5' untranslated region adipose derived stem cell adipose mesenchymal stem cell Article cell fate controlled study DNA sequence DNA transposition gene knockdown genetic transcription human human cell mesenchymal stem cell nerve cell differentiation priority journal protein DNA interaction retroposon SH-SY5Y cell line upregulation Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11. © 2018 Federation of European Biochemical Societies JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic human adipose-derived stem cells
LINE-1
neuroblastoma
neuronal differentiation
SOX-11
DNA
LINE1 DNA
transcription factor Sox11
unclassified drug
5' untranslated region
adipose derived stem cell
adipose mesenchymal stem cell
Article
cell fate
controlled study
DNA sequence
DNA transposition
gene knockdown
genetic transcription
human
human cell
mesenchymal stem cell
nerve cell differentiation
priority journal
protein DNA interaction
retroposon
SH-SY5Y cell line
upregulation
spellingShingle human adipose-derived stem cells
LINE-1
neuroblastoma
neuronal differentiation
SOX-11
DNA
LINE1 DNA
transcription factor Sox11
unclassified drug
5' untranslated region
adipose derived stem cell
adipose mesenchymal stem cell
Article
cell fate
controlled study
DNA sequence
DNA transposition
gene knockdown
genetic transcription
human
human cell
mesenchymal stem cell
nerve cell differentiation
priority journal
protein DNA interaction
retroposon
SH-SY5Y cell line
upregulation
Orqueda, A.J.
Gatti, C.R.
Ogara, M.F.
Falzone, T.L.
SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
topic_facet human adipose-derived stem cells
LINE-1
neuroblastoma
neuronal differentiation
SOX-11
DNA
LINE1 DNA
transcription factor Sox11
unclassified drug
5' untranslated region
adipose derived stem cell
adipose mesenchymal stem cell
Article
cell fate
controlled study
DNA sequence
DNA transposition
gene knockdown
genetic transcription
human
human cell
mesenchymal stem cell
nerve cell differentiation
priority journal
protein DNA interaction
retroposon
SH-SY5Y cell line
upregulation
description Activity of the human long interspersed nuclear elements-1 (LINE-1) retrotransposon occurs mainly in early embryonic development and during hippocampal neurogenesis. SOX-11, a transcription factor relevant to neuronal development, has unknown functions in the control of LINE-1 retrotransposon activity during neuronal differentiation. To study the dependence of LINE-1 activity on SOX-11 during neuronal differentiation, we induced differentiation of human SH-SY5Y neuroblastoma cells and adult adipose mesenchymal stem cells (hASCs) to a neuronal fate and found increased LINE-1 activity. We also show that SOX-11 protein binding to the LINE-1 promoter is higher in differentiating neuroblastoma cells, while knock-down of SOX-11 inhibits the induction of LINE-1 transcription in differentiating conditions. These results suggest that activation of LINE-1 retrotransposition during neuronal differentiation is mediated by SOX-11. © 2018 Federation of European Biochemical Societies
format JOUR
author Orqueda, A.J.
Gatti, C.R.
Ogara, M.F.
Falzone, T.L.
author_facet Orqueda, A.J.
Gatti, C.R.
Ogara, M.F.
Falzone, T.L.
author_sort Orqueda, A.J.
title SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
title_short SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
title_full SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
title_fullStr SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
title_full_unstemmed SOX-11 regulates LINE-1 retrotransposon activity during neuronal differentiation
title_sort sox-11 regulates line-1 retrotransposon activity during neuronal differentiation
url http://hdl.handle.net/20.500.12110/paper_00145793_v592_n22_p3708_Orqueda
work_keys_str_mv AT orquedaaj sox11regulatesline1retrotransposonactivityduringneuronaldifferentiation
AT gatticr sox11regulatesline1retrotransposonactivityduringneuronaldifferentiation
AT ogaramf sox11regulatesline1retrotransposonactivityduringneuronaldifferentiation
AT falzonetl sox11regulatesline1retrotransposonactivityduringneuronaldifferentiation
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