Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties
Previous works proposed that aquaporins behave as mechanosensitive channels. However, principal issues about mechanosensitivity of aquaporins are not known. In this work, we characterized the mechanosensitive properties of the water channels BvTIP1;2 (TIP1) and BvPIP2;1 (PIP2) from red beet (Beta vu...
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todo:paper_00145793_v591_n11_p1555_Goldman2023-10-03T14:13:13Z Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties Goldman, R.P. Jozefkowicz, C. Canessa Fortuna, A. Sutka, M. Alleva, K. Ozu, M. aquaporins mechanosensitivity membrane tension volumetric elastic modulus water flux water permeability aquaporin PIP2 protein TIP1 protein unclassified drug aquaporin plant protein adult animal cell beet controlled study female heterologous expression Letter mechanotransduction nonhuman oocyte osmotic pressure priority journal water permeability water transport Xenopus laevis Young modulus cell membrane genetics metabolism osmosis physiology Aquaporins Beta vulgaris Cell Membrane Osmosis Plant Proteins Previous works proposed that aquaporins behave as mechanosensitive channels. However, principal issues about mechanosensitivity of aquaporins are not known. In this work, we characterized the mechanosensitive properties of the water channels BvTIP1;2 (TIP1) and BvPIP2;1 (PIP2) from red beet (Beta vulgaris). We simultaneously measured the mechanical behavior and the water transport rates during the osmotic response of emptied-out oocytes expressing TIP1 or PIP2. Our results indicate that TIP1 is a mechanosensitive aquaporin, whereas PIP2 is not. We found that a single exponential function between the osmotic permeability coefficient and the volumetric elastic modulus governs the mechanosensitivity of TIP1. Finally, homology modeling analysis indicates that putative residues involved in mechanosensitivity show different quantity and distribution in TIP1 and PIP2. © 2017 Federation of European Biochemical Societies Fil:Sutka, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Ozu, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v591_n11_p1555_Goldman |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
aquaporins mechanosensitivity membrane tension volumetric elastic modulus water flux water permeability aquaporin PIP2 protein TIP1 protein unclassified drug aquaporin plant protein adult animal cell beet controlled study female heterologous expression Letter mechanotransduction nonhuman oocyte osmotic pressure priority journal water permeability water transport Xenopus laevis Young modulus cell membrane genetics metabolism osmosis physiology Aquaporins Beta vulgaris Cell Membrane Osmosis Plant Proteins |
| spellingShingle |
aquaporins mechanosensitivity membrane tension volumetric elastic modulus water flux water permeability aquaporin PIP2 protein TIP1 protein unclassified drug aquaporin plant protein adult animal cell beet controlled study female heterologous expression Letter mechanotransduction nonhuman oocyte osmotic pressure priority journal water permeability water transport Xenopus laevis Young modulus cell membrane genetics metabolism osmosis physiology Aquaporins Beta vulgaris Cell Membrane Osmosis Plant Proteins Goldman, R.P. Jozefkowicz, C. Canessa Fortuna, A. Sutka, M. Alleva, K. Ozu, M. Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| topic_facet |
aquaporins mechanosensitivity membrane tension volumetric elastic modulus water flux water permeability aquaporin PIP2 protein TIP1 protein unclassified drug aquaporin plant protein adult animal cell beet controlled study female heterologous expression Letter mechanotransduction nonhuman oocyte osmotic pressure priority journal water permeability water transport Xenopus laevis Young modulus cell membrane genetics metabolism osmosis physiology Aquaporins Beta vulgaris Cell Membrane Osmosis Plant Proteins |
| description |
Previous works proposed that aquaporins behave as mechanosensitive channels. However, principal issues about mechanosensitivity of aquaporins are not known. In this work, we characterized the mechanosensitive properties of the water channels BvTIP1;2 (TIP1) and BvPIP2;1 (PIP2) from red beet (Beta vulgaris). We simultaneously measured the mechanical behavior and the water transport rates during the osmotic response of emptied-out oocytes expressing TIP1 or PIP2. Our results indicate that TIP1 is a mechanosensitive aquaporin, whereas PIP2 is not. We found that a single exponential function between the osmotic permeability coefficient and the volumetric elastic modulus governs the mechanosensitivity of TIP1. Finally, homology modeling analysis indicates that putative residues involved in mechanosensitivity show different quantity and distribution in TIP1 and PIP2. © 2017 Federation of European Biochemical Societies |
| format |
JOUR |
| author |
Goldman, R.P. Jozefkowicz, C. Canessa Fortuna, A. Sutka, M. Alleva, K. Ozu, M. |
| author_facet |
Goldman, R.P. Jozefkowicz, C. Canessa Fortuna, A. Sutka, M. Alleva, K. Ozu, M. |
| author_sort |
Goldman, R.P. |
| title |
Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| title_short |
Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| title_full |
Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| title_fullStr |
Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| title_full_unstemmed |
Tonoplast (BvTIP1;2) and plasma membrane (BvPIP2;1) aquaporins show different mechanosensitive properties |
| title_sort |
tonoplast (bvtip1;2) and plasma membrane (bvpip2;1) aquaporins show different mechanosensitive properties |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v591_n11_p1555_Goldman |
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