Agonist mobility on supported lipid bilayers affects Fas mediated death response

Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobili...

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Autores principales:	Sánchez, M.F., Levi, V., Weidemann, T., Carrer, D.C.
Formato:	JOUR
Materias:	Apoptosis Fas receptor Fluorescence correlation spectroscopy Functionalized surface Supported lipid bilayer Fas antibody Fas antigen Fas ligand FAS protein, human lipid bilayer agonist mobility apoptosis Article concentration (parameters) controlled study correlational study fluorescence correlation spectroscopy HEK293 cell line human human cell lipid composition membrane fluidity molecular biology priority journal quantitative analysis supported lipid bilayer z scan fluorescence correlation spectroscopy agonists metabolism movement (physiology) Antigens, CD95 HEK293 Cells Humans Lipid Bilayers Membrane Fluidity Movement
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v589_n23_p3527_Sanchez
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00145793_v589_n23_p3527_Sanchez
record_format	dspace
spelling	todo:paper_00145793_v589_n23_p3527_Sanchez2023-10-03T14:13:12Z Agonist mobility on supported lipid bilayers affects Fas mediated death response Sánchez, M.F. Levi, V. Weidemann, T. Carrer, D.C. Apoptosis Fas receptor Fluorescence correlation spectroscopy Functionalized surface Supported lipid bilayer Fas antibody Fas antigen Fas ligand Fas antigen FAS protein, human lipid bilayer agonist mobility apoptosis Article concentration (parameters) controlled study correlational study fluorescence correlation spectroscopy HEK293 cell line human human cell lipid bilayer lipid composition membrane fluidity molecular biology priority journal quantitative analysis supported lipid bilayer z scan fluorescence correlation spectroscopy agonists lipid bilayer membrane fluidity metabolism movement (physiology) Antigens, CD95 Apoptosis HEK293 Cells Humans Lipid Bilayers Membrane Fluidity Movement Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer. © 2015 Federation of European Biochemical Societies. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v589_n23_p3527_Sanchez
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Apoptosis Fas receptor Fluorescence correlation spectroscopy Functionalized surface Supported lipid bilayer Fas antibody Fas antigen Fas ligand Fas antigen FAS protein, human lipid bilayer agonist mobility apoptosis Article concentration (parameters) controlled study correlational study fluorescence correlation spectroscopy HEK293 cell line human human cell lipid bilayer lipid composition membrane fluidity molecular biology priority journal quantitative analysis supported lipid bilayer z scan fluorescence correlation spectroscopy agonists lipid bilayer membrane fluidity metabolism movement (physiology) Antigens, CD95 Apoptosis HEK293 Cells Humans Lipid Bilayers Membrane Fluidity Movement
spellingShingle	Apoptosis Fas receptor Fluorescence correlation spectroscopy Functionalized surface Supported lipid bilayer Fas antibody Fas antigen Fas ligand Fas antigen FAS protein, human lipid bilayer agonist mobility apoptosis Article concentration (parameters) controlled study correlational study fluorescence correlation spectroscopy HEK293 cell line human human cell lipid bilayer lipid composition membrane fluidity molecular biology priority journal quantitative analysis supported lipid bilayer z scan fluorescence correlation spectroscopy agonists lipid bilayer membrane fluidity metabolism movement (physiology) Antigens, CD95 Apoptosis HEK293 Cells Humans Lipid Bilayers Membrane Fluidity Movement Sánchez, M.F. Levi, V. Weidemann, T. Carrer, D.C. Agonist mobility on supported lipid bilayers affects Fas mediated death response
topic_facet	Apoptosis Fas receptor Fluorescence correlation spectroscopy Functionalized surface Supported lipid bilayer Fas antibody Fas antigen Fas ligand Fas antigen FAS protein, human lipid bilayer agonist mobility apoptosis Article concentration (parameters) controlled study correlational study fluorescence correlation spectroscopy HEK293 cell line human human cell lipid bilayer lipid composition membrane fluidity molecular biology priority journal quantitative analysis supported lipid bilayer z scan fluorescence correlation spectroscopy agonists lipid bilayer membrane fluidity metabolism movement (physiology) Antigens, CD95 Apoptosis HEK293 Cells Humans Lipid Bilayers Membrane Fluidity Movement
description	Extrinsic apoptosis is initiated by recognition and clustering of the single-pass transmembrane proteins Fas ligand and Fas expressed at the surface of closely apposed lymphocytes and target cells, respectively. Since Fas-mediated death response was mainly studied with soluble antibodies, the mobility constraints for receptor activation by a membrane embedded agonist is not well understood. We explored this influence by stimulating apoptosis on functionalized supported lipid bilayers, where we quantified agonist mobility by z-scan fluorescence correlation spectroscopy. Using different lipid compositions, we show that the apoptotic response correlates with increased lateral mobility of the agonist in the lipid bilayer. © 2015 Federation of European Biochemical Societies.
format	JOUR
author	Sánchez, M.F. Levi, V. Weidemann, T. Carrer, D.C.
author_facet	Sánchez, M.F. Levi, V. Weidemann, T. Carrer, D.C.
author_sort	Sánchez, M.F.
title	Agonist mobility on supported lipid bilayers affects Fas mediated death response
title_short	Agonist mobility on supported lipid bilayers affects Fas mediated death response
title_full	Agonist mobility on supported lipid bilayers affects Fas mediated death response
title_fullStr	Agonist mobility on supported lipid bilayers affects Fas mediated death response
title_full_unstemmed	Agonist mobility on supported lipid bilayers affects Fas mediated death response
title_sort	agonist mobility on supported lipid bilayers affects fas mediated death response
url	http://hdl.handle.net/20.500.12110/paper_00145793_v589_n23_p3527_Sanchez
work_keys_str_mv	AT sanchezmf agonistmobilityonsupportedlipidbilayersaffectsfasmediateddeathresponse AT leviv agonistmobilityonsupportedlipidbilayersaffectsfasmediateddeathresponse AT weidemannt agonistmobilityonsupportedlipidbilayersaffectsfasmediateddeathresponse AT carrerdc agonistmobilityonsupportedlipidbilayersaffectsfasmediateddeathresponse
_version_	1807315857660968960

Agonist mobility on supported lipid bilayers affects Fas mediated death response

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