Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport

The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishi...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	De Rossi, M.C., De Rossi, M.E., Sued, M., Rodríguez, D., Bruno, L., Levi, V.
Formato:	JOUR
Materias:	Intracellular transport Molecular motors Single particle tracking Xenopus laevis melanophores cytoplasmic dynein kinesin 2 molecular motor kinesin Xenopus protein animal cell Article cell aggregation cell culture cell organelle cell transport elasticity mathematical model melanophore melanosome microtubule nonhuman priority journal Xenopus laevis animal metabolism transport at the cellular level Animals Biological Transport Cytoplasmic Dyneins Kinesin Melanosomes Xenopus Proteins
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v589_n19_p2763_DeRossi
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00145793_v589_n19_p2763_DeRossi
record_format	dspace
spelling	todo:paper_00145793_v589_n19_p2763_DeRossi2023-10-03T14:13:11Z Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport De Rossi, M.C. De Rossi, M.E. Sued, M. Rodríguez, D. Bruno, L. Levi, V. Intracellular transport Molecular motors Single particle tracking Xenopus laevis melanophores cytoplasmic dynein kinesin 2 molecular motor cytoplasmic dynein kinesin Xenopus protein animal cell Article cell aggregation cell culture cell organelle cell transport elasticity mathematical model melanophore melanosome microtubule nonhuman priority journal Xenopus laevis animal melanosome metabolism transport at the cellular level Animals Biological Transport Cytoplasmic Dyneins Kinesin Melanosomes Xenopus laevis Xenopus Proteins The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecular linker. We determined both the stiffness of this linker and the organelles speed and observed that these parameters depended on the organelle size and cargo direction. Our results suggest that melanosome transport is driven by two dissimilar teams: whereas dynein motors compete with kinesin-2 affecting the properties of plus-end directed organelles, kinesin-2 does not seem to play a similar role during minus-end transport. © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved. Fil:De Rossi, M.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Sued, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Rodríguez, D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bruno, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Levi, V. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v589_n19_p2763_DeRossi
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Intracellular transport Molecular motors Single particle tracking Xenopus laevis melanophores cytoplasmic dynein kinesin 2 molecular motor cytoplasmic dynein kinesin Xenopus protein animal cell Article cell aggregation cell culture cell organelle cell transport elasticity mathematical model melanophore melanosome microtubule nonhuman priority journal Xenopus laevis animal melanosome metabolism transport at the cellular level Animals Biological Transport Cytoplasmic Dyneins Kinesin Melanosomes Xenopus laevis Xenopus Proteins
spellingShingle	Intracellular transport Molecular motors Single particle tracking Xenopus laevis melanophores cytoplasmic dynein kinesin 2 molecular motor cytoplasmic dynein kinesin Xenopus protein animal cell Article cell aggregation cell culture cell organelle cell transport elasticity mathematical model melanophore melanosome microtubule nonhuman priority journal Xenopus laevis animal melanosome metabolism transport at the cellular level Animals Biological Transport Cytoplasmic Dyneins Kinesin Melanosomes Xenopus laevis Xenopus Proteins De Rossi, M.C. De Rossi, M.E. Sued, M. Rodríguez, D. Bruno, L. Levi, V. Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
topic_facet	Intracellular transport Molecular motors Single particle tracking Xenopus laevis melanophores cytoplasmic dynein kinesin 2 molecular motor cytoplasmic dynein kinesin Xenopus protein animal cell Article cell aggregation cell culture cell organelle cell transport elasticity mathematical model melanophore melanosome microtubule nonhuman priority journal Xenopus laevis animal melanosome metabolism transport at the cellular level Animals Biological Transport Cytoplasmic Dyneins Kinesin Melanosomes Xenopus laevis Xenopus Proteins
description	The mechanisms involved in bidirectional transport along microtubules remain largely unknown. We explored the collective action of kinesin-2 and dynein motors during transport of melanosomes in Xenopus laevis melanophores. These motors are attached to organelles through accessory proteins establishing a complex molecular linker. We determined both the stiffness of this linker and the organelles speed and observed that these parameters depended on the organelle size and cargo direction. Our results suggest that melanosome transport is driven by two dissimilar teams: whereas dynein motors compete with kinesin-2 affecting the properties of plus-end directed organelles, kinesin-2 does not seem to play a similar role during minus-end transport. © 2015 Federation of European Biochemical Societies. Published by Elsevier B.V. All rights reserved.
format	JOUR
author	De Rossi, M.C. De Rossi, M.E. Sued, M. Rodríguez, D. Bruno, L. Levi, V.
author_facet	De Rossi, M.C. De Rossi, M.E. Sued, M. Rodríguez, D. Bruno, L. Levi, V.
author_sort	De Rossi, M.C.
title	Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
title_short	Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
title_full	Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
title_fullStr	Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
title_full_unstemmed	Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
title_sort	asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport
url	http://hdl.handle.net/20.500.12110/paper_00145793_v589_n19_p2763_DeRossi
work_keys_str_mv	AT derossimc asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport AT derossime asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport AT suedm asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport AT rodriguezd asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport AT brunol asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport AT leviv asymmetriesinkinesin2andcytoplasmicdyneincontributionstomelanosometransport
_version_	1782026342149128192

Asymmetries in kinesin-2 and cytoplasmic dynein contributions to melanosome transport

Ejemplares similares