Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication

L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Pereira, C.A., Alonso, G.D., Ivaldi, S., Silber, A., Alves, M.J.M., Bouvier, L.A., Flawiá, M.M., Torres, H.N.
Formato:	JOUR
Materias:	Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00145793_v526_n1-3_p111_Pereira
record_format	dspace
spelling	todo:paper_00145793_v526_n1-3_p111_Pereira2023-10-03T14:13:08Z Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication Pereira, C.A. Alonso, G.D. Ivaldi, S. Silber, A. Alves, M.J.M. Bouvier, L.A. Flawiá, M.M. Torres, H.N. Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell replication rate reduction. The use of conditioned or fresh medium mimics uptake variations. Interestingly, in non-replicative trypomastigote cells, L-arginine uptake was undetectable. The association between L-arginine uptake and cell replication was demonstrated using the antimitotic agent hydroxyurea. Arginine kinase, the enzyme responsible for phosphoarginine and ATP synthesis, also shows a differential activity in epimastigote and trypomastigote parasite stages. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
spellingShingle	Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi Pereira, C.A. Alonso, G.D. Ivaldi, S. Silber, A. Alves, M.J.M. Bouvier, L.A. Flawiá, M.M. Torres, H.N. Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
topic_facet	Arginine kinase Arginine transport Arginine uptake Phosphoarginine Trypanosoma cruzi adenosine triphosphate antimitotic agent arginine arginine kinase hydroxyurea amino acid metabolism article cell division enzyme activity epimastigote life cycle nonhuman polyacrylamide gel electrophoresis priority journal synthesis Trypanosoma cruzi trypomastigote Western blotting Animals Arginine Arginine Kinase Biological Transport Life Cycle Stages Trypanosoma cruzi Trypanosoma Trypanosoma cruzi
description	L-Arginine plays an essential role in the energetic metabolism of Trypanosoma cruzi. In this work we propose a relationship between L-arginine uptake, arginine kinase activity and the parasite replication ability. In epimastigote cultures L-arginine uptake decreases continuously accompanying a cell replication rate reduction. The use of conditioned or fresh medium mimics uptake variations. Interestingly, in non-replicative trypomastigote cells, L-arginine uptake was undetectable. The association between L-arginine uptake and cell replication was demonstrated using the antimitotic agent hydroxyurea. Arginine kinase, the enzyme responsible for phosphoarginine and ATP synthesis, also shows a differential activity in epimastigote and trypomastigote parasite stages. © 2002 Published by Elsevier Science B.V. on behalf of the Federation of European Biochemical Societies.
format	JOUR
author	Pereira, C.A. Alonso, G.D. Ivaldi, S. Silber, A. Alves, M.J.M. Bouvier, L.A. Flawiá, M.M. Torres, H.N.
author_facet	Pereira, C.A. Alonso, G.D. Ivaldi, S. Silber, A. Alves, M.J.M. Bouvier, L.A. Flawiá, M.M. Torres, H.N.
author_sort	Pereira, C.A.
title	Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
title_short	Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
title_full	Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
title_fullStr	Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
title_full_unstemmed	Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication
title_sort	arginine metabolism in trypanosoma cruzi is coupled to parasite stage and replication
url	http://hdl.handle.net/20.500.12110/paper_00145793_v526_n1-3_p111_Pereira
work_keys_str_mv	AT pereiraca argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT alonsogd argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT ivaldis argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT silbera argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT alvesmjm argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT bouvierla argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT flawiamm argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication AT torreshn argininemetabolismintrypanosomacruziiscoupledtoparasitestageandreplication
_version_	1782026586960166912

Arginine metabolism in Trypanosoma cruzi is coupled to parasite stage and replication

Ejemplares similares