Spermidine is essential for normal proliferation of trypanosomatid protozoa
Trypanosomatid parasites containing a metabolically unstable ornithine decarboxylase (ODC) are naturally resistant to high levels of α-difluoromethylornithine (DFMO) because this ODC inhibitor, though causing a drastic reduction of intracellular putrescine, elicits only a moderate decrease of the sp...
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todo:paper_00145793_v508_n3_p323_Gonzalez2023-10-03T14:13:08Z Spermidine is essential for normal proliferation of trypanosomatid protozoa González, N.S. Huber, A. Algranati, I.D. Cyclohexylamine Polyamine Spermidine Spermidine synthase Trypanosomatid proliferation eflornithine eticyclidine ornithine decarboxylase putrescine spermidine article cell proliferation controlled study Crithidia fasciculata enzyme stability nonhuman priority journal protozoon Trypanosoma cruzi Animals Crithidia fasciculata Cyclohexylamines Eflornithine Enzyme Inhibitors Ornithine Decarboxylase Putrescine Spermidine Spermidine Synthase Spermine Trypanosoma cruzi Protozoa Trypanosomatidae Trypanosomatid parasites containing a metabolically unstable ornithine decarboxylase (ODC) are naturally resistant to high levels of α-difluoromethylornithine (DFMO) because this ODC inhibitor, though causing a drastic reduction of intracellular putrescine, elicits only a moderate decrease of the spermidine endogenous pool. In this study we have used a combination of DFMO with cyclohexylamine (CHA; bis-cyclohexylammonium sulfate), an inhibitor of spermidine synthase, to reach a more complete depletion of spermidine. Under these conditions we have observed the arrest of proliferation not only in trypanosomatids with stable ODC but also in parasites with an enzyme of high turnover rate. In all cases the reinitiation of proliferation occurred only after the addition of exogenous spermidine, and neither putrescine nor spermine were able to induce the same effect. © 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. Fil:González, N.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Algranati, I.D. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v508_n3_p323_Gonzalez |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Cyclohexylamine Polyamine Spermidine Spermidine synthase Trypanosomatid proliferation eflornithine eticyclidine ornithine decarboxylase putrescine spermidine article cell proliferation controlled study Crithidia fasciculata enzyme stability nonhuman priority journal protozoon Trypanosoma cruzi Animals Crithidia fasciculata Cyclohexylamines Eflornithine Enzyme Inhibitors Ornithine Decarboxylase Putrescine Spermidine Spermidine Synthase Spermine Trypanosoma cruzi Protozoa Trypanosomatidae |
| spellingShingle |
Cyclohexylamine Polyamine Spermidine Spermidine synthase Trypanosomatid proliferation eflornithine eticyclidine ornithine decarboxylase putrescine spermidine article cell proliferation controlled study Crithidia fasciculata enzyme stability nonhuman priority journal protozoon Trypanosoma cruzi Animals Crithidia fasciculata Cyclohexylamines Eflornithine Enzyme Inhibitors Ornithine Decarboxylase Putrescine Spermidine Spermidine Synthase Spermine Trypanosoma cruzi Protozoa Trypanosomatidae González, N.S. Huber, A. Algranati, I.D. Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| topic_facet |
Cyclohexylamine Polyamine Spermidine Spermidine synthase Trypanosomatid proliferation eflornithine eticyclidine ornithine decarboxylase putrescine spermidine article cell proliferation controlled study Crithidia fasciculata enzyme stability nonhuman priority journal protozoon Trypanosoma cruzi Animals Crithidia fasciculata Cyclohexylamines Eflornithine Enzyme Inhibitors Ornithine Decarboxylase Putrescine Spermidine Spermidine Synthase Spermine Trypanosoma cruzi Protozoa Trypanosomatidae |
| description |
Trypanosomatid parasites containing a metabolically unstable ornithine decarboxylase (ODC) are naturally resistant to high levels of α-difluoromethylornithine (DFMO) because this ODC inhibitor, though causing a drastic reduction of intracellular putrescine, elicits only a moderate decrease of the spermidine endogenous pool. In this study we have used a combination of DFMO with cyclohexylamine (CHA; bis-cyclohexylammonium sulfate), an inhibitor of spermidine synthase, to reach a more complete depletion of spermidine. Under these conditions we have observed the arrest of proliferation not only in trypanosomatids with stable ODC but also in parasites with an enzyme of high turnover rate. In all cases the reinitiation of proliferation occurred only after the addition of exogenous spermidine, and neither putrescine nor spermine were able to induce the same effect. © 2001 Federation of European Biochemical Societies. Published by Elsevier Science B.V. All rights reserved. |
| format |
JOUR |
| author |
González, N.S. Huber, A. Algranati, I.D. |
| author_facet |
González, N.S. Huber, A. Algranati, I.D. |
| author_sort |
González, N.S. |
| title |
Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| title_short |
Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| title_full |
Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| title_fullStr |
Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| title_full_unstemmed |
Spermidine is essential for normal proliferation of trypanosomatid protozoa |
| title_sort |
spermidine is essential for normal proliferation of trypanosomatid protozoa |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v508_n3_p323_Gonzalez |
| work_keys_str_mv |
AT gonzalezns spermidineisessentialfornormalproliferationoftrypanosomatidprotozoa AT hubera spermidineisessentialfornormalproliferationoftrypanosomatidprotozoa AT algranatiid spermidineisessentialfornormalproliferationoftrypanosomatidprotozoa |
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