Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells
Silencing of fibronectin (FN) expression seems to be one of the key mechanisms underlying metastatic behaviour. An inverse correlation exists between FN expression levels and the metastatic potential of two related murine mammary adenocarcinomas, M3 and MM3. Primary cultures of M3 tumour, which is m...
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| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00145793_v440_n3_p277_Werbajh |
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todo:paper_00145793_v440_n3_p277_Werbajh2023-10-03T14:13:05Z Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells Werbajh, S.E. Urtreger, A.J. Puricelli, L.I. De Lustig, E.S. Bal De Kier Joffé, E. Kornblihtt, A.R. Fibronectin Metastasis Transcription fibronectin messenger RNA article breast adenocarcinoma human human cell lung metastasis metastasis potential priority journal promoter region protein expression RNA transcription single strand conformation polymorphism Murinae Silencing of fibronectin (FN) expression seems to be one of the key mechanisms underlying metastatic behaviour. An inverse correlation exists between FN expression levels and the metastatic potential of two related murine mammary adenocarcinomas, M3 and MM3. Primary cultures of M3 tumour, which is moderately metastatic to lung (40% incidence), show a conspicuous FN extracellular matrix (ECM) and high levels of FN mRNA, while primary cultures of the highly metastatic MM3 tumour (95% lung incidence) are negative for FN in immunofluorescence and show at least 40-fold lower levels of FN mRNA, only detectable by RT-PCR, with a different pattern of alternatively spliced EDI isoforms compared to M3 cells. We show that the FN promoter sequence is not altered in MM3 cells. Transfection experiments with CAT constructs indicate that silencing occurs at the transcriptional level, involving the 220-bp proximal promoter region. Copyright (C) 1998 Federation of European Biochemical Societies. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00145793_v440_n3_p277_Werbajh |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Fibronectin Metastasis Transcription fibronectin messenger RNA article breast adenocarcinoma human human cell lung metastasis metastasis potential priority journal promoter region protein expression RNA transcription single strand conformation polymorphism Murinae |
| spellingShingle |
Fibronectin Metastasis Transcription fibronectin messenger RNA article breast adenocarcinoma human human cell lung metastasis metastasis potential priority journal promoter region protein expression RNA transcription single strand conformation polymorphism Murinae Werbajh, S.E. Urtreger, A.J. Puricelli, L.I. De Lustig, E.S. Bal De Kier Joffé, E. Kornblihtt, A.R. Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| topic_facet |
Fibronectin Metastasis Transcription fibronectin messenger RNA article breast adenocarcinoma human human cell lung metastasis metastasis potential priority journal promoter region protein expression RNA transcription single strand conformation polymorphism Murinae |
| description |
Silencing of fibronectin (FN) expression seems to be one of the key mechanisms underlying metastatic behaviour. An inverse correlation exists between FN expression levels and the metastatic potential of two related murine mammary adenocarcinomas, M3 and MM3. Primary cultures of M3 tumour, which is moderately metastatic to lung (40% incidence), show a conspicuous FN extracellular matrix (ECM) and high levels of FN mRNA, while primary cultures of the highly metastatic MM3 tumour (95% lung incidence) are negative for FN in immunofluorescence and show at least 40-fold lower levels of FN mRNA, only detectable by RT-PCR, with a different pattern of alternatively spliced EDI isoforms compared to M3 cells. We show that the FN promoter sequence is not altered in MM3 cells. Transfection experiments with CAT constructs indicate that silencing occurs at the transcriptional level, involving the 220-bp proximal promoter region. Copyright (C) 1998 Federation of European Biochemical Societies. |
| format |
JOUR |
| author |
Werbajh, S.E. Urtreger, A.J. Puricelli, L.I. De Lustig, E.S. Bal De Kier Joffé, E. Kornblihtt, A.R. |
| author_facet |
Werbajh, S.E. Urtreger, A.J. Puricelli, L.I. De Lustig, E.S. Bal De Kier Joffé, E. Kornblihtt, A.R. |
| author_sort |
Werbajh, S.E. |
| title |
Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| title_short |
Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| title_full |
Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| title_fullStr |
Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| title_full_unstemmed |
Downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| title_sort |
downregulation of fibronectin transcription in highly metastatic adenocarcinoma cells |
| url |
http://hdl.handle.net/20.500.12110/paper_00145793_v440_n3_p277_Werbajh |
| work_keys_str_mv |
AT werbajhse downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells AT urtregeraj downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells AT puricellili downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells AT delustiges downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells AT baldekierjoffee downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells AT kornblihttar downregulationoffibronectintranscriptioninhighlymetastaticadenocarcinomacells |
| _version_ |
1782025963018649600 |