Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor
ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surfac...
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todo:paper_00144894_v82_n3_p290_Abuin2023-10-03T14:12:48Z Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor Abuin, G. Couto, A.S. De Lederkremer, R.M. Casal, O.L. Galli, C. Colli, W. Alves, M.J.M. Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc. Fil:Couto, A.S. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:De Lederkremer, R.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Casal, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144894_v82_n3_p290_Abuin |
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Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi |
| spellingShingle |
Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi Abuin, G. Couto, A.S. De Lederkremer, R.M. Casal, O.L. Galli, C. Colli, W. Alves, M.J.M. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| topic_facet |
Trypanosoma Trypanosoma cruzi glycoprotein inositol membrane protein animal experiment article controlled study high performance liquid chromatography immunoprecipitation nonhuman polyacrylamide gel electrophoresis priority journal thin layer chromatography trypanosoma cruzi trypomastigote Animals Antibodies, Monoclonal Blotting, Western Chromatography, Thin Layer Glycoproteins Glycosylphosphatidylinositols Membrane Proteins Precipitin Tests Protozoan Proteins Trypanosoma cruzi |
| description |
ABUIN, G., COUTO, A. S., DE LEDERKREMER, R. M., CASAL, O. L., GALLI, C., COLLI, W., AND ALVES, M. J. M. 1996. Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor. Experimental Parasitology 82, 290-297. Tc-85, an 85-kDa surface glycoprotein specific for the trypomastigote stage of Trypanosoma cruzi, has been implicated in the invasion of host cells by the parasite. Radioactive palmitic acid was incorporated into Tc-85 immunoprecipitated from the culture medium with the H1A10 monoclonal antibody, suggesting that shedding occurs with Tc-85 bearing its GPI anchor. In contrast to the glycoprotein remaining in the parasites, the glycosylphosphatidylinositol moiety in shed Tc-85 is resistant to phosphatidylinositol phospholipase C and becomes susceptible to the enzyme following alkali treatment. An alkylglycerol was identified by thin layer chromatography of an ether extract after the enzymatic reaction. Resistance to cleavage by phospholipase C is due to fatty acid esterification of the inositol residue in shed Tc-85. This is the first example of inositol modification in anchors from a glycoprotein of Trypanosoma cruzi. © 1996 Academic Press, Inc. |
| format |
JOUR |
| author |
Abuin, G. Couto, A.S. De Lederkremer, R.M. Casal, O.L. Galli, C. Colli, W. Alves, M.J.M. |
| author_facet |
Abuin, G. Couto, A.S. De Lederkremer, R.M. Casal, O.L. Galli, C. Colli, W. Alves, M.J.M. |
| author_sort |
Abuin, G. |
| title |
Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| title_short |
Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| title_full |
Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| title_fullStr |
Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| title_full_unstemmed |
Trypanosoma cruzi: The Tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| title_sort |
trypanosoma cruzi: the tc-85 surface glycoprotein shed by trypomastigotes bears a modified glycosylphosphatidylinositol anchor |
| url |
http://hdl.handle.net/20.500.12110/paper_00144894_v82_n3_p290_Abuin |
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