Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg)
The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of th...
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todo:paper_00144894_v117_n2_p188_Guida2023-10-03T14:12:47Z Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) Güida, M.C. Esteva, M.I. Camino, A. Flawiá, M.M. Torres, H.N. Paveto, C. Epigallocatechin gallate PCD Programmed cell death Trypanocide Chagas' disease epigallocatechin gallate animal experiment animal model animal tissue apoptosis article Chagas disease controlled study drug mechanism fragmentation reaction growth inhibition histopathology in vitro study in vivo study male mouse nonhuman parasitemia priority journal survival rate Trypanosoma cruzi trypomastigote Animals Antioxidants Catechin Chagas Disease Disease Models, Animal DNA Fragmentation Dose-Response Relationship, Drug Hepatocytes In Situ Nick-End Labeling Male Mice Mice, Inbred BALB C Parasitemia Random Allocation Trypanocidal Agents Trypanosoma cruzi Animalia Murinae Mus Trypanosoma cruzi The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 μM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3 mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. © 2007 Elsevier Inc. All rights reserved. Fil:Güida, M.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Flawiá, M.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Torres, H.N. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Paveto, C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144894_v117_n2_p188_Guida |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
Epigallocatechin gallate PCD Programmed cell death Trypanocide Chagas' disease epigallocatechin gallate animal experiment animal model animal tissue apoptosis article Chagas disease controlled study drug mechanism fragmentation reaction growth inhibition histopathology in vitro study in vivo study male mouse nonhuman parasitemia priority journal survival rate Trypanosoma cruzi trypomastigote Animals Antioxidants Catechin Chagas Disease Disease Models, Animal DNA Fragmentation Dose-Response Relationship, Drug Hepatocytes In Situ Nick-End Labeling Male Mice Mice, Inbred BALB C Parasitemia Random Allocation Trypanocidal Agents Trypanosoma cruzi Animalia Murinae Mus Trypanosoma cruzi |
| spellingShingle |
Epigallocatechin gallate PCD Programmed cell death Trypanocide Chagas' disease epigallocatechin gallate animal experiment animal model animal tissue apoptosis article Chagas disease controlled study drug mechanism fragmentation reaction growth inhibition histopathology in vitro study in vivo study male mouse nonhuman parasitemia priority journal survival rate Trypanosoma cruzi trypomastigote Animals Antioxidants Catechin Chagas Disease Disease Models, Animal DNA Fragmentation Dose-Response Relationship, Drug Hepatocytes In Situ Nick-End Labeling Male Mice Mice, Inbred BALB C Parasitemia Random Allocation Trypanocidal Agents Trypanosoma cruzi Animalia Murinae Mus Trypanosoma cruzi Güida, M.C. Esteva, M.I. Camino, A. Flawiá, M.M. Torres, H.N. Paveto, C. Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| topic_facet |
Epigallocatechin gallate PCD Programmed cell death Trypanocide Chagas' disease epigallocatechin gallate animal experiment animal model animal tissue apoptosis article Chagas disease controlled study drug mechanism fragmentation reaction growth inhibition histopathology in vitro study in vivo study male mouse nonhuman parasitemia priority journal survival rate Trypanosoma cruzi trypomastigote Animals Antioxidants Catechin Chagas Disease Disease Models, Animal DNA Fragmentation Dose-Response Relationship, Drug Hepatocytes In Situ Nick-End Labeling Male Mice Mice, Inbred BALB C Parasitemia Random Allocation Trypanocidal Agents Trypanosoma cruzi Animalia Murinae Mus Trypanosoma cruzi |
| description |
The trypanocidal activity of catechins on Trypanosoma cruzi bloodstream trypomastigotes has been previously reported. Herein, we present the effect of epigallocatechin gallate (EGCg) on parasitemia and survival in a murine model of acute Chagas' disease as well as on the epimastigote form of the parasite. Upon intraperitoneal administration of daily doses of 0.8 mg/kg/day of EGCg for 45 days, mice survival rates increased from 11% to 60%, while parasitemia diminished to 50%. No side effects were observed in EGCg-treated animals. Fifty percent inhibition of epimastigotes growth was achieved with 311 μM EGCg 120 h after drug addition. No lysis, total culture growth inhibition or morphological changes were observed upon addition of 1-3 mM EGCg at 24 h. This treatment also produced oligosomal fragmentation of epimastigotes DNA, suggesting a programmed cell death (PCD)-like process. All these findings point out EGCg as a potential new lead compound for chemotherapy of Chagas' disease. © 2007 Elsevier Inc. All rights reserved. |
| format |
JOUR |
| author |
Güida, M.C. Esteva, M.I. Camino, A. Flawiá, M.M. Torres, H.N. Paveto, C. |
| author_facet |
Güida, M.C. Esteva, M.I. Camino, A. Flawiá, M.M. Torres, H.N. Paveto, C. |
| author_sort |
Güida, M.C. |
| title |
Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| title_short |
Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| title_full |
Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| title_fullStr |
Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| title_full_unstemmed |
Trypanosoma cruzi: In vitro and in vivo antiproliferative effects of epigallocatechin gallate (EGCg) |
| title_sort |
trypanosoma cruzi: in vitro and in vivo antiproliferative effects of epigallocatechin gallate (egcg) |
| url |
http://hdl.handle.net/20.500.12110/paper_00144894_v117_n2_p188_Guida |
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