Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process

We have previously reported that in the distal stump of ligated sciatic nerves, there is a change in the distribution of myelin basic protein (MBP) and P 0 protein immunoreactivities. These results agreed with the studies of myelin isolated from the distal stump of animals submitted to ligation of t...

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Autores principales:	Setton-Avruj, C.P., Musolino, P.L., Salis, C., Alló, M., Bizzozero, O., Villar, M.J., Soto, E.F., Pasquini, J.M.
Formato:	JOUR
Materias:	α-Globin Bone marrow mononuclear cells Macrophages Myelin proteins Peripheral myelin Wallerian degeneration alpha globin beta globin glycine dehydrogenase (decarboxylating) isoprotein messenger RNA myelin myelin basic protein peptide animal cell animal experiment animal model animal tissue article bone marrow cell cell isolation cell migration controlled study electrophoretic mobility erythroid cell immunoreactivity macrophage nerve cell differentiation nerve degeneration nerve injury nerve ligation nerve regeneration nonhuman polyacrylamide gel electrophoresis priority journal protein degradation protein isolation rat real time polymerase chain reaction Schwann cell sciatic nerve Western blotting Animals Blotting, Western Bone Marrow Cells Cell Movement Electrophoresis, Polyacrylamide Gel Female Globins Immunohistochemistry Male Myelin Basic Proteins Nerve Degeneration Nerve Regeneration Nuclease Protection Assays Peptides Peripheral Nerves Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Schwann Cells Sciatic Nerve Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00144886_v203_n2_p568_SettonAvruj
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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spelling	todo:paper_00144886_v203_n2_p568_SettonAvruj2023-10-03T14:12:44Z Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process Setton-Avruj, C.P. Musolino, P.L. Salis, C. Alló, M. Bizzozero, O. Villar, M.J. Soto, E.F. Pasquini, J.M. α-Globin Bone marrow mononuclear cells Macrophages Myelin proteins Peripheral myelin Wallerian degeneration alpha globin beta globin glycine dehydrogenase (decarboxylating) isoprotein messenger RNA myelin myelin basic protein peptide animal cell animal experiment animal model animal tissue article bone marrow cell cell isolation cell migration controlled study electrophoretic mobility erythroid cell immunoreactivity macrophage nerve cell differentiation nerve degeneration nerve injury nerve ligation nerve regeneration nonhuman polyacrylamide gel electrophoresis priority journal protein degradation protein isolation rat real time polymerase chain reaction Schwann cell sciatic nerve Western blotting Animals Blotting, Western Bone Marrow Cells Cell Movement Electrophoresis, Polyacrylamide Gel Female Globins Immunohistochemistry Male Myelin Basic Proteins Nerve Degeneration Nerve Regeneration Nuclease Protection Assays Peptides Peripheral Nerves Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Schwann Cells Sciatic Nerve Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin We have previously reported that in the distal stump of ligated sciatic nerves, there is a change in the distribution of myelin basic protein (MBP) and P 0 protein immunoreactivities. These results agreed with the studies of myelin isolated from the distal stump of animals submitted to ligation of the sciatic nerve, showing a gradual increase in a 14 kDa band with an electrophoretic mobility similar to that of an MBP isoform, among other changes. This band, which was resolved into two bands of 14 and 15 kDa using a 16% gel, was found to contain a mixture of MBP fragments and peptides with great homology with α- and β-globins. In agreement with these results, we have demonstrated that the mRNA of α-globin is present in the proximal and distal stumps of the ligated nerve. It is also detected at very low levels in Schwann cells isolated from normal nerves. These results could be due to the presence of α- and/or β-globin arising from immature cells of the erythroid series. Also, they could be present in macrophages, which spontaneously migrate to the injured nerve to promote the degradation of myelin proteins. Cells isolated from normal adult rat bone marrow which were injected intraortically were found to migrate to the injured area. These cells could contribute to the remyelination of the damaged area participating in the removal of myelin debris, through their transdifferentiation into Schwann cells or through their fusion with preexisting Schwann cells in the distal stump of the injured sciatic nerve. © 2006 Elsevier Inc. All rights reserved. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144886_v203_n2_p568_SettonAvruj
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	α-Globin Bone marrow mononuclear cells Macrophages Myelin proteins Peripheral myelin Wallerian degeneration alpha globin beta globin glycine dehydrogenase (decarboxylating) isoprotein messenger RNA myelin myelin basic protein peptide animal cell animal experiment animal model animal tissue article bone marrow cell cell isolation cell migration controlled study electrophoretic mobility erythroid cell immunoreactivity macrophage nerve cell differentiation nerve degeneration nerve injury nerve ligation nerve regeneration nonhuman polyacrylamide gel electrophoresis priority journal protein degradation protein isolation rat real time polymerase chain reaction Schwann cell sciatic nerve Western blotting Animals Blotting, Western Bone Marrow Cells Cell Movement Electrophoresis, Polyacrylamide Gel Female Globins Immunohistochemistry Male Myelin Basic Proteins Nerve Degeneration Nerve Regeneration Nuclease Protection Assays Peptides Peripheral Nerves Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Schwann Cells Sciatic Nerve Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin
spellingShingle	α-Globin Bone marrow mononuclear cells Macrophages Myelin proteins Peripheral myelin Wallerian degeneration alpha globin beta globin glycine dehydrogenase (decarboxylating) isoprotein messenger RNA myelin myelin basic protein peptide animal cell animal experiment animal model animal tissue article bone marrow cell cell isolation cell migration controlled study electrophoretic mobility erythroid cell immunoreactivity macrophage nerve cell differentiation nerve degeneration nerve injury nerve ligation nerve regeneration nonhuman polyacrylamide gel electrophoresis priority journal protein degradation protein isolation rat real time polymerase chain reaction Schwann cell sciatic nerve Western blotting Animals Blotting, Western Bone Marrow Cells Cell Movement Electrophoresis, Polyacrylamide Gel Female Globins Immunohistochemistry Male Myelin Basic Proteins Nerve Degeneration Nerve Regeneration Nuclease Protection Assays Peptides Peripheral Nerves Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Schwann Cells Sciatic Nerve Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin Setton-Avruj, C.P. Musolino, P.L. Salis, C. Alló, M. Bizzozero, O. Villar, M.J. Soto, E.F. Pasquini, J.M. Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
topic_facet	α-Globin Bone marrow mononuclear cells Macrophages Myelin proteins Peripheral myelin Wallerian degeneration alpha globin beta globin glycine dehydrogenase (decarboxylating) isoprotein messenger RNA myelin myelin basic protein peptide animal cell animal experiment animal model animal tissue article bone marrow cell cell isolation cell migration controlled study electrophoretic mobility erythroid cell immunoreactivity macrophage nerve cell differentiation nerve degeneration nerve injury nerve ligation nerve regeneration nonhuman polyacrylamide gel electrophoresis priority journal protein degradation protein isolation rat real time polymerase chain reaction Schwann cell sciatic nerve Western blotting Animals Blotting, Western Bone Marrow Cells Cell Movement Electrophoresis, Polyacrylamide Gel Female Globins Immunohistochemistry Male Myelin Basic Proteins Nerve Degeneration Nerve Regeneration Nuclease Protection Assays Peptides Peripheral Nerves Rats Rats, Wistar Reverse Transcriptase Polymerase Chain Reaction RNA, Messenger Schwann Cells Sciatic Nerve Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization Trypsin
description	We have previously reported that in the distal stump of ligated sciatic nerves, there is a change in the distribution of myelin basic protein (MBP) and P 0 protein immunoreactivities. These results agreed with the studies of myelin isolated from the distal stump of animals submitted to ligation of the sciatic nerve, showing a gradual increase in a 14 kDa band with an electrophoretic mobility similar to that of an MBP isoform, among other changes. This band, which was resolved into two bands of 14 and 15 kDa using a 16% gel, was found to contain a mixture of MBP fragments and peptides with great homology with α- and β-globins. In agreement with these results, we have demonstrated that the mRNA of α-globin is present in the proximal and distal stumps of the ligated nerve. It is also detected at very low levels in Schwann cells isolated from normal nerves. These results could be due to the presence of α- and/or β-globin arising from immature cells of the erythroid series. Also, they could be present in macrophages, which spontaneously migrate to the injured nerve to promote the degradation of myelin proteins. Cells isolated from normal adult rat bone marrow which were injected intraortically were found to migrate to the injured area. These cells could contribute to the remyelination of the damaged area participating in the removal of myelin debris, through their transdifferentiation into Schwann cells or through their fusion with preexisting Schwann cells in the distal stump of the injured sciatic nerve. © 2006 Elsevier Inc. All rights reserved.
format	JOUR
author	Setton-Avruj, C.P. Musolino, P.L. Salis, C. Alló, M. Bizzozero, O. Villar, M.J. Soto, E.F. Pasquini, J.M.
author_facet	Setton-Avruj, C.P. Musolino, P.L. Salis, C. Alló, M. Bizzozero, O. Villar, M.J. Soto, E.F. Pasquini, J.M.
author_sort	Setton-Avruj, C.P.
title	Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
title_short	Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
title_full	Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
title_fullStr	Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
title_full_unstemmed	Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
title_sort	presence of α-globin mrna and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process
url	http://hdl.handle.net/20.500.12110/paper_00144886_v203_n2_p568_SettonAvruj
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Presence of α-globin mRNA and migration of bone marrow cells after sciatic nerve injury suggests their participation in the degeneration/regeneration process

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