Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants
The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the...
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todo:paper_00144827_v181_n1_p116_Resnicoff2023-10-03T14:12:36Z Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants Resnicoff, M. Medrano, E.E. 13 cis retinal butyric acid estradiol insulin prostaglandin f2 alpha radioisotope thrombin unclassified drug breast cancer cell culture cell differentiation cell growth cell strain mcf 7 controlled study cytology female human human cell priority journal Breast Neoplasms Butyric Acid Butyric Acids Cell Differentiation Cell Division Cell Survival Culture Media Dinoprost DNA Estradiol Hormones Human Insulin Retinaldehyde Retinoids Support, Non-U.S. Gov't Thrombin Tumor Cells, Cultured Tumor Stem Cells The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2a, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2a has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate. © 1989. Fil:Resnicoff, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144827_v181_n1_p116_Resnicoff |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
13 cis retinal butyric acid estradiol insulin prostaglandin f2 alpha radioisotope thrombin unclassified drug breast cancer cell culture cell differentiation cell growth cell strain mcf 7 controlled study cytology female human human cell priority journal Breast Neoplasms Butyric Acid Butyric Acids Cell Differentiation Cell Division Cell Survival Culture Media Dinoprost DNA Estradiol Hormones Human Insulin Retinaldehyde Retinoids Support, Non-U.S. Gov't Thrombin Tumor Cells, Cultured Tumor Stem Cells |
| spellingShingle |
13 cis retinal butyric acid estradiol insulin prostaglandin f2 alpha radioisotope thrombin unclassified drug breast cancer cell culture cell differentiation cell growth cell strain mcf 7 controlled study cytology female human human cell priority journal Breast Neoplasms Butyric Acid Butyric Acids Cell Differentiation Cell Division Cell Survival Culture Media Dinoprost DNA Estradiol Hormones Human Insulin Retinaldehyde Retinoids Support, Non-U.S. Gov't Thrombin Tumor Cells, Cultured Tumor Stem Cells Resnicoff, M. Medrano, E.E. Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| topic_facet |
13 cis retinal butyric acid estradiol insulin prostaglandin f2 alpha radioisotope thrombin unclassified drug breast cancer cell culture cell differentiation cell growth cell strain mcf 7 controlled study cytology female human human cell priority journal Breast Neoplasms Butyric Acid Butyric Acids Cell Differentiation Cell Division Cell Survival Culture Media Dinoprost DNA Estradiol Hormones Human Insulin Retinaldehyde Retinoids Support, Non-U.S. Gov't Thrombin Tumor Cells, Cultured Tumor Stem Cells |
| description |
The human breast tumor cell line was separated by Percoll density gradient centrifugation into six different subpopulations, A to F, one of which (E) appears to contain the stem cells on the basis of several criteria (M. Resnicoff et al. 1987, Proc. Natl. Acad. Sci. USA84, 7295. We now analyzed the response of the isolated subpopulations to insulin, thrombin, PGF2a, estradiol, and 13-cis-retinal. We demonstrate that the first two growth factors stimulate [3H]thymidine incorporation in the more differentiated subpopulations (D and F), while PGF2a has mitogenic activity in subpopulations C and D. In the absence of any added growth factor, estradiol has the extreme and transient capacity of allowing the stem cell to detach from the tissue culture dish and to grow in suspension as multicellular aggregates (MCF-7/SE cells). 13-cis-Retinal acts as a negative modulator of differentiation and protects the cells from the inhibitory and differentiation activity of Na-butyrate. © 1989. |
| format |
JOUR |
| author |
Resnicoff, M. Medrano, E.E. |
| author_facet |
Resnicoff, M. Medrano, E.E. |
| author_sort |
Resnicoff, M. |
| title |
Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| title_short |
Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| title_full |
Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| title_fullStr |
Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| title_full_unstemmed |
Growth factors and hormones which affect survival, growth, and differentiation of the MCF-7 stem cells and their descendants |
| title_sort |
growth factors and hormones which affect survival, growth, and differentiation of the mcf-7 stem cells and their descendants |
| url |
http://hdl.handle.net/20.500.12110/paper_00144827_v181_n1_p116_Resnicoff |
| work_keys_str_mv |
AT resnicoffm growthfactorsandhormoneswhichaffectsurvivalgrowthanddifferentiationofthemcf7stemcellsandtheirdescendants AT medranoee growthfactorsandhormoneswhichaffectsurvivalgrowthanddifferentiationofthemcf7stemcellsandtheirdescendants |
| _version_ |
1782025817856933888 |