Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation

The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to...

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Detalles Bibliográficos
Autores principales: Podhajcer, O.L., Resnicoff, M., Bover, L., Medrano, E.E., Slavutsky, I., Larripa, I., Mordoh, J.
Formato: JOUR
Materias:
estradiol
tamoxifen
anchorage independent growth
cell culture
cell growth
cell strain mcf 7
controlled study
cytology
female
human
human cell
karyotyping
priority journal
stem cell
ultrastructure
Breast Neoplasms
Cell Adhesion
Cell Division
Cell Line
Estradiol
Genetic Markers
Human
Karyotyping
Support, Non-U.S. Gov't
Tamoxifen
Tumor Stem Cells
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
Descripción
Sumario:The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988.

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