Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation
The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to...
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todo:paper_00144827_v179_n1_p58_Podhajcer2023-10-03T14:12:36Z Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation Podhajcer, O.L. Resnicoff, M. Bover, L. Medrano, E.E. Slavutsky, I. Larripa, I. Mordoh, J. estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988. Fil:Podhajcer, O.L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Resnicoff, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Bover, L. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Medrano, E.E. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Larripa, I. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
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Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells |
| spellingShingle |
estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells Podhajcer, O.L. Resnicoff, M. Bover, L. Medrano, E.E. Slavutsky, I. Larripa, I. Mordoh, J. Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| topic_facet |
estradiol tamoxifen anchorage independent growth cell culture cell growth cell strain mcf 7 controlled study cytology female human human cell karyotyping priority journal stem cell ultrastructure Breast Neoplasms Cell Adhesion Cell Division Cell Line Estradiol Genetic Markers Human Karyotyping Support, Non-U.S. Gov't Tamoxifen Tumor Stem Cells |
| description |
The MCF-7 breast carcinoma cell line can be separated by Percoll density gradient centrifugation into several subpopulations, A to F, one of which (E) has been previously suggested to be highly enriched in stem cells. The anchorage-independent growth of the different fractions and its sensitivity to estradiol (E2) and tamoxifen (TAM) was assayed. The anchorage-independent growth capacity of the different fractions was E>A>B> D>C,F. The E fraction had the highest clonogenic index (6.62 ± 1.18) and was unaffected by E2 or TAM. The karyotypic analysis of the E fraction revealed features similar to those of the unfractionated cell line. It is suggested that the high growth rate of fraction E is due to an enrichment in stem cells and not to the existence of a different clone. © 1988. |
| format |
JOUR |
| author |
Podhajcer, O.L. Resnicoff, M. Bover, L. Medrano, E.E. Slavutsky, I. Larripa, I. Mordoh, J. |
| author_facet |
Podhajcer, O.L. Resnicoff, M. Bover, L. Medrano, E.E. Slavutsky, I. Larripa, I. Mordoh, J. |
| author_sort |
Podhajcer, O.L. |
| title |
Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| title_short |
Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| title_full |
Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| title_fullStr |
Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| title_full_unstemmed |
Effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from MCF-7 breast carcinoma cells: Cytogenetic analysis of the stem cell subpopulation |
| title_sort |
effect of estradiol and tamoxifen on the anchorage-independent growth of the subpopulations derived from mcf-7 breast carcinoma cells: cytogenetic analysis of the stem cell subpopulation |
| url |
http://hdl.handle.net/20.500.12110/paper_00144827_v179_n1_p58_Podhajcer |
| work_keys_str_mv |
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