Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin

The growth of the human metastatic cell line (T-47D) in a chemically defined medium (DM) is shown to be dependent on the presence of three traditional growth factors: epidermal growth factor, insulin, and transferrin. The addition of thrombin further stimulates its growth. The mitogenic action on a...

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Autores principales: Medrano, E.E., Cafferata, E.G.A., Larcher, F.
Formato: JOUR
Materias:
growth factor
thrombin
cell growth
human
human cell
metastasis
protein synthesis
tumor cell
Breast Neoplasms
Cell Division
Culture Media
DNA Replication
Drug Synergism
Epidermal Growth Factor
Gene Expression Regulation
Human
Insulin
Neoplasm Proteins
Phosphorylation
Sepharose
Support, Non-U.S. Gov't
Thrombin
Transferrin
Tumor Cells, Cultured
Acceso en línea:http://hdl.handle.net/20.500.12110/paper_00144827_v172_n2_p354_Medrano
Aporte de:
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires
id todo:paper_00144827_v172_n2_p354_Medrano
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spelling todo:paper_00144827_v172_n2_p354_Medrano2023-10-03T14:12:35Z Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin Medrano, E.E. Cafferata, E.G.A. Larcher, F. growth factor thrombin cell growth human human cell metastasis protein synthesis tumor cell Breast Neoplasms Cell Division Culture Media DNA Replication Drug Synergism Epidermal Growth Factor Gene Expression Regulation Human Insulin Neoplasm Proteins Phosphorylation Sepharose Support, Non-U.S. Gov't Thrombin Transferrin Tumor Cells, Cultured The growth of the human metastatic cell line (T-47D) in a chemically defined medium (DM) is shown to be dependent on the presence of three traditional growth factors: epidermal growth factor, insulin, and transferrin. The addition of thrombin further stimulates its growth. The mitogenic action on a human mammary tumor cell line from epithelial origin is a novel action of thrombin. Cells in the DM show striking morphological changes which are dramatically enhanced by the addition of thrombin. These observations are part of a pleiotropic response to the growth factors: the protein content of the cells increases in the defined medium; the 2DG gels of the 35S- and 32P-labeled proteins show important changes in spots, several of which are probably of cytoskeletal origin. It is also shown that cells in a semisolid growth factor-supplemented medium have growth advantages over their counterparts grown with serum. All the phenotypic changes mentioned above reveal the important role of growth factors in the growth and behavior of this mammary cell line. The results obtained with thrombin indicate a new site of action of this enzyme which may be important in the metastatic spread of human mammary tumor cells. © 1987. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00144827_v172_n2_p354_Medrano
institution Universidad de Buenos Aires
institution_str I-28
repository_str R-134
collection Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic growth factor
thrombin
cell growth
human
human cell
metastasis
protein synthesis
tumor cell
Breast Neoplasms
Cell Division
Culture Media
DNA Replication
Drug Synergism
Epidermal Growth Factor
Gene Expression Regulation
Human
Insulin
Neoplasm Proteins
Phosphorylation
Sepharose
Support, Non-U.S. Gov't
Thrombin
Transferrin
Tumor Cells, Cultured
spellingShingle growth factor
thrombin
cell growth
human
human cell
metastasis
protein synthesis
tumor cell
Breast Neoplasms
Cell Division
Culture Media
DNA Replication
Drug Synergism
Epidermal Growth Factor
Gene Expression Regulation
Human
Insulin
Neoplasm Proteins
Phosphorylation
Sepharose
Support, Non-U.S. Gov't
Thrombin
Transferrin
Tumor Cells, Cultured
Medrano, E.E.
Cafferata, E.G.A.
Larcher, F.
Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
topic_facet growth factor
thrombin
cell growth
human
human cell
metastasis
protein synthesis
tumor cell
Breast Neoplasms
Cell Division
Culture Media
DNA Replication
Drug Synergism
Epidermal Growth Factor
Gene Expression Regulation
Human
Insulin
Neoplasm Proteins
Phosphorylation
Sepharose
Support, Non-U.S. Gov't
Thrombin
Transferrin
Tumor Cells, Cultured
description The growth of the human metastatic cell line (T-47D) in a chemically defined medium (DM) is shown to be dependent on the presence of three traditional growth factors: epidermal growth factor, insulin, and transferrin. The addition of thrombin further stimulates its growth. The mitogenic action on a human mammary tumor cell line from epithelial origin is a novel action of thrombin. Cells in the DM show striking morphological changes which are dramatically enhanced by the addition of thrombin. These observations are part of a pleiotropic response to the growth factors: the protein content of the cells increases in the defined medium; the 2DG gels of the 35S- and 32P-labeled proteins show important changes in spots, several of which are probably of cytoskeletal origin. It is also shown that cells in a semisolid growth factor-supplemented medium have growth advantages over their counterparts grown with serum. All the phenotypic changes mentioned above reveal the important role of growth factors in the growth and behavior of this mammary cell line. The results obtained with thrombin indicate a new site of action of this enzyme which may be important in the metastatic spread of human mammary tumor cells. © 1987.
format JOUR
author Medrano, E.E.
Cafferata, E.G.A.
Larcher, F.
author_facet Medrano, E.E.
Cafferata, E.G.A.
Larcher, F.
author_sort Medrano, E.E.
title Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
title_short Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
title_full Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
title_fullStr Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
title_full_unstemmed Role of thrombin in the proliferative response of T-47D mammary tumor cells. Mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
title_sort role of thrombin in the proliferative response of t-47d mammary tumor cells. mitogenic action and pleiotropic modifications induced together with epidermal growth factor and insulin
url http://hdl.handle.net/20.500.12110/paper_00144827_v172_n2_p354_Medrano
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AT cafferataega roleofthrombinintheproliferativeresponseoft47dmammarytumorcellsmitogenicactionandpleiotropicmodificationsinducedtogetherwithepidermalgrowthfactorandinsulin
AT larcherf roleofthrombinintheproliferativeresponseoft47dmammarytumorcellsmitogenicactionandpleiotropicmodificationsinducedtogetherwithepidermalgrowthfactorandinsulin
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