Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands

The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of th...

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Autores principales:	Tomio, J.M., Grinstein, M.
Formato:	JOUR
Materias:	amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00142956_v6_n1_p80_Tomio
record_format	dspace
spelling	todo:paper_00142956_v6_n1_p80_Tomio2023-10-03T14:11:56Z Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands Tomio, J.M. Grinstein, M. amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of the Harderian glands with δ‐aminolevulinic acid and porphobilinogen, varying conditions such as temperature, time, aero‐or anaerobiosis and cofactor and substrate concentration, porphyrins with a carboxyl content ranging from 8–2 were formed. Thus, the presence of δ‐aminolevulinate dehydratase, porphobilinogenase, decarboxylase and coproporphyrinogenase systems were demonstrated. Copyright © 1968, Wiley Blackwell. All rights reserved Fil:Tomio, J.M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:Grinstein, M. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates
spellingShingle	amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates Tomio, J.M. Grinstein, M. Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
topic_facet	amino acid carbon coenzyme A glycine levulinic acid ligase porphyrin succinic acid derivative animal article biosynthesis enzymology lacrimal apparatus male metabolism mitochondrion nictitating membrane rat Amino Acids Animal Carbon Isotopes Coenzyme A Glycine Lacrimal Apparatus Levulinic Acids Ligases Male Mitochondria Nictitating Membrane Porphyrins Rats Succinates
description	The enzymic system that catalyzes the total synthesis of protoporphyrin IX has been found in the Harderian gland of the rat. The presence of the enzyme δ‐aminolevulinic acid synthetase was demonstrated in mitochondria by the formation of [14C]δ‐aminolevulinic acid. On incubation of homogenates of the Harderian glands with δ‐aminolevulinic acid and porphobilinogen, varying conditions such as temperature, time, aero‐or anaerobiosis and cofactor and substrate concentration, porphyrins with a carboxyl content ranging from 8–2 were formed. Thus, the presence of δ‐aminolevulinate dehydratase, porphobilinogenase, decarboxylase and coproporphyrinogenase systems were demonstrated. Copyright © 1968, Wiley Blackwell. All rights reserved
format	JOUR
author	Tomio, J.M. Grinstein, M.
author_facet	Tomio, J.M. Grinstein, M.
author_sort	Tomio, J.M.
title	Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
title_short	Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
title_full	Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
title_fullStr	Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
title_full_unstemmed	Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands
title_sort	porphyrin biosynthesis: 5. biosynthesis of protoporphyrin ix in harderian glands
url	http://hdl.handle.net/20.500.12110/paper_00142956_v6_n1_p80_Tomio
work_keys_str_mv	AT tomiojm porphyrinbiosynthesis5biosynthesisofprotoporphyrinixinharderianglands AT grinsteinm porphyrinbiosynthesis5biosynthesisofprotoporphyrinixinharderianglands
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Porphyrin Biosynthesis: 5. Biosynthesis of Protoporphyrin IX in Harderian Glands

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