Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars
The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solu...
| Autores principales: | , , |
|---|---|
| Formato: | JOUR |
| Materias: | |
| Acceso en línea: | http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia |
| Aporte de: |
| id |
todo:paper_00142956_v43_n1_p93_Garcia |
|---|---|
| record_format |
dspace |
| spelling |
todo:paper_00142956_v43_n1_p93_Garcia2023-10-03T14:11:56Z Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars GarcÍA, R.C. Recondo, E. Dankert, M. lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia |
| institution |
Universidad de Buenos Aires |
| institution_str |
I-28 |
| repository_str |
R-134 |
| collection |
Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) |
| topic |
lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars |
| spellingShingle |
lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars GarcÍA, R.C. Recondo, E. Dankert, M. Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| topic_facet |
lipid magnesium manganese polysaccharide uridine diphosphate glucose acetobacter xylinum in vitro study microorganism theoretical study Acetobacter Animal Borohydrides Butanols Carbon Radioisotopes Cell Division Chromatography, DEAE-Cellulose Chromatography, Ion Exchange Chromatography, Paper Electrophoresis, Paper Galactose Glucose Hexokinase Kinetics Lipids Magnesium Manganese Muscles Phosphoglucomutase Phosphorus Radioisotopes Polysaccharides, Bacterial Rabbits Saccharomyces cerevisiae Solubility Time Factors Uridine Diphosphate Sugars |
| description |
The incorporation of radioactivity in vitro from UDP‐[14C]glucose into endogenous acceptors in a crude enzymatic preparation from Acetobacter xylinum is described. A fraction of the total incorporated radioactivity could be extracted with organic solvents, like butanol. The remainder was mostly solubilized by hot alkali, leaving an insoluble fraction. No incorporation was observed in the absence of magnesium ions. Mn2+ could only partially replace Mg2+, and had an inhibitory effect when both ions were present. Analysis of the butanol‐soluble fraction permitted the isolation of at least three components characterized as: lipid‐diphosphate α‐glucose, lipid‐diphosphate α‐cellobiose and lipid‐monophosphate β‐galactose. In the presence of [β‐32P]UDP‐glucose the system incorporated radioactivity only into the diphosphate derivatives, indicating the transfer of a α‐glucose 1‐phosphate group from UDP‐glucose. Experiments with doubly labelled substrate confirmed these results. The role these compounds might play in polysaccharide biosynthesis is discussed. Copyright © 1974, Wiley Blackwell. All rights reserved |
| format |
JOUR |
| author |
GarcÍA, R.C. Recondo, E. Dankert, M. |
| author_facet |
GarcÍA, R.C. Recondo, E. Dankert, M. |
| author_sort |
GarcÍA, R.C. |
| title |
Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| title_short |
Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| title_full |
Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| title_fullStr |
Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| title_full_unstemmed |
Polysaccharide Biosynthesis in Acetobacter xylinum Enzymatic Synthesis of Lipid Diphosphate and Monophospate Sugars |
| title_sort |
polysaccharide biosynthesis in acetobacter xylinum enzymatic synthesis of lipid diphosphate and monophospate sugars |
| url |
http://hdl.handle.net/20.500.12110/paper_00142956_v43_n1_p93_Garcia |
| work_keys_str_mv |
AT garciarc polysaccharidebiosynthesisinacetobacterxylinumenzymaticsynthesisoflipiddiphosphateandmonophospatesugars AT recondoe polysaccharidebiosynthesisinacetobacterxylinumenzymaticsynthesisoflipiddiphosphateandmonophospatesugars AT dankertm polysaccharidebiosynthesisinacetobacterxylinumenzymaticsynthesisoflipiddiphosphateandmonophospatesugars |
| _version_ |
1807322569438658560 |