Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts

Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoi...

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Detalles Bibliográficos
Autores principales:	CÁCERES, J.F., BLANGY, D., GLIKIN, G.C.
Formato:	JOUR
Materias:	DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

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