Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts

Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoi...

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Autores principales:	CÁCERES, J.F., BLANGY, D., GLIKIN, G.C.
Formato:	JOUR
Materias:	DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't
Acceso en línea:	http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES
Aporte de:	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA) de Universidad de Buenos Aires

id	todo:paper_00142956_v181_n2_p531_CACERES
record_format	dspace
spelling	todo:paper_00142956_v181_n2_p531_CACERES2023-10-03T14:11:51Z Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts CÁCERES, J.F. BLANGY, D. GLIKIN, G.C. DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved Fil:CÁCERES, J.F. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. Fil:GLIKIN, G.C. Universidad de Buenos Aires. Facultad de Ciencias Exactas y Naturales; Argentina. JOUR info:eu-repo/semantics/openAccess http://creativecommons.org/licenses/by/2.5/ar http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES
institution	Universidad de Buenos Aires
institution_str	I-28
repository_str	R-134
collection	Biblioteca Digital - Facultad de Ciencias Exactas y Naturales (UBA)
topic	DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't
spellingShingle	DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't CÁCERES, J.F. BLANGY, D. GLIKIN, G.C. Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
topic_facet	DNA DNA topoisomerase DNA topoisomerase (ATP hydrolysing) animal article cell culture chromatin DNA supercoiling isolation and purification kinetics metabolism mouse Animal Cells, Cultured Chromatin DNA DNA Topoisomerases, Type I DNA Topoisomerases, Type II DNA, Superhelical Kinetics Mice Support, Non-U.S. Gov't
description	Nuclear extracts of 3T6 mouse cells were able to assemble in vitro minichromosomes which displayed a 150‐bp periodicity. Activities of both DNA topoisomerases I and II were detected in these extracts. When a supercoiled pUC DNA was added, it was first relaxed in less than 3 min, then slowly supercoiled again in 1–4 h. Both reactions occurred either in the absence or the presence of added Mg2+ and/or ATP, they were not blocked by DNA topoisomerase II inhibitors and they were inhibited by an antiserum against DNA topoisomerase I and by camptothecin. These findings led us to propose that, under our in vitro assay conditions, chromatin assembly is mainly carried out by a DNA topoisomerase I. Copyright © 1989, Wiley Blackwell. All rights reserved
format	JOUR
author	CÁCERES, J.F. BLANGY, D. GLIKIN, G.C.
author_facet	CÁCERES, J.F. BLANGY, D. GLIKIN, G.C.
author_sort	CÁCERES, J.F.
title	Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
title_short	Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
title_full	Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
title_fullStr	Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
title_full_unstemmed	Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts
title_sort	requirement of dna topoisomerases for in vitro chromatin assembly by 3t6 mouse cell extracts
url	http://hdl.handle.net/20.500.12110/paper_00142956_v181_n2_p531_CACERES
work_keys_str_mv	AT caceresjf requirementofdnatopoisomerasesforinvitrochromatinassemblyby3t6mousecellextracts AT blangyd requirementofdnatopoisomerasesforinvitrochromatinassemblyby3t6mousecellextracts AT glikingc requirementofdnatopoisomerasesforinvitrochromatinassemblyby3t6mousecellextracts
_version_	1807315318836559872

Requirement of DNA topoisomerases for in vitro chromatin assembly by 3T6 mouse cell extracts

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